INVESTIGATION OF PHYTOCHEMICALS IN METHANOLIC HERBA EXTRACT OF SILENE RUSCIFOLIA BY LC-QTOF/MS AND GC/MS

Kenan Can Tok; Muhammed Mesud Hürkul; Nazmiye Neslihan Bozkurt; Ayhan İbrahim Aysal; Şeyda Yayla

doi:10.33483/jfpau.1133615

Research Article

INVESTIGATION OF PHYTOCHEMICALS IN METHANOLIC HERBA EXTRACT OF SILENE RUSCIFOLIA BY LC-QTOF/MS AND GC/MS

Year 2022, Volume: 46 Issue: 3, 827 - 838, 30.09.2022

Kenan Can Tok , Muhammed Mesud Hürkul , Nazmiye Neslihan Bozkurt , Ayhan İbrahim Aysal , Şeyda Yayla

https://doi.org/10.33483/jfpau.1133615

Abstract

Objective: Silene L. (Caryophyllaceae) species are traditionally used for the treatment of inflammation, urinary inflammation, eye trouble, skin problem, stomach ache, dysentery, tooth decay, fever, headache, malaria, pimples and backache. Chemical ingredients of Silene species consist of flavonoids, anthocyanidins, terpenoids, triterpene saponins, phytoecdysteroids, benzenoids, vitamins, and show antioxidant, anti-inflammatory, antitumor and antiviral activities. Silene ruscifolia (Hub.-Mor. & Reese) Hub.-Mor. is called "gizli nakıl" in Türkiye.
Material and Method: The plant material was collected from Beynam Forest (Ankara/Türkiye). The aerial parts of the plant were extracted with methanol in an ultrasonic bath. HPLC system (Agilent 1260 Series) with an autosampler, binary pump, column oven, and a UV detector was coupled with an iFunnel Quadrupole Time-of-Flight LC-MS system (Agilent G6550A) with a Dual Spray Agilent Jet Stream electrospray ionization source. Agilent TC C-18 (4.6 mm x 150 mm x 5 µm) column was used for the separation of the compounds. The GC-MS analysis of extract was performed using an Agilent 6890 gas chromatograph equipped with an Agilent 5973N quadrupole mass spectrometer (Agilent, USA). Mass Hunter software (Qualitative Analysis B.07.00) and the NIST Mass Spectral Library (2014) were used to determine and identify the compounds.
Result and Discussion: LC-MS Q-TOF analysis showed that S. ruscifolia contained rutin, narcissin, luteolin, isorhamnetin, rhamnetin and quercetin dimethyl ether. GC-MS analysis showed that the extract had the highest content of sugar (50.5%) and sugar alcohols (46.39%). It also contains carboxylic acid (0.47%), fatty acid (0.64%), sugar acid (0.42%), glycoside (0.48%), carotenoids (0.61%) and benzoic acid ester (0.49%). D-pinitol has the highest content in the extract with 41.14%.

Keywords

GC/MS, Herba, LC-QTOF/MS, Silene ruscifolia

References

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SILENE RUSCIFOLIA METANOLİK HERBA EKSTRESİNDEKİ FİTOKİMYASALLARIN LC-QTOF/MS VE GC/MS İLE İNCELENMESİ

Year 2022, Volume: 46 Issue: 3, 827 - 838, 30.09.2022

Kenan Can Tok , Muhammed Mesud Hürkul , Nazmiye Neslihan Bozkurt , Ayhan İbrahim Aysal , Şeyda Yayla

https://doi.org/10.33483/jfpau.1133615

Abstract

Amaç: Silene L. (Caryophyllaceae) türleri geleneksel olarak iltihap, idrar yolu iltihabı, göz rahatsızlığı, cilt sorunu, mide ağrısı, dizanteri, diş çürümesi, ateş, baş ağrısı, sıtma, sivilce ve sırt ağrısı tedavisinde kullanılmaktadır. Silene türlerinin kimyasal bileşenleri flavonoidler, antosiyanidinler, terpenoidler, triterpen saponinler, fitoekdisteroidler, benzenoidler, vitaminlerden oluşur ve antioksidan, antiinflamatuar, antitümör, antiviral aktivite gösterirler. Silene ruscifolia (Hub.-Mor. & Reese) Hub.-Mor. Türkiye'de "gizli nakıl" olarak adlandırılır.
Gereç ve Yöntem: Bitki materyali Beynam Ormanı'ndan (Ankara/Türkiye) toplandı. Bitkinin toprak üstü kısımları ultrasonik banyoda metanol ile ekstre edildi. Otomatik örnekleyici, ikili pompa, kolon fırını ve bir UV dedektörüne sahip HPLC sistemi (Agilent 1260 Serisi), Çift Sprey Agilent Jet Stream elektrosprey iyonizasyon kaynağına sahip bir iFunnel Quadrupole Time-of-Flight LC-MS system (Agilent G6550A) ile birleştirildi. Bileşiklerin ayrılması için Agilent TC C-18 (4.6 mm x 150 mm x 5 µm) kolonu kullanıldı. Ekstrenin GC-MS analizi, bir Agilent 5973N dört kutuplu kütle spektrometresi (Agilent, ABD) ile donatılmış bir Agilent 6890 gaz kromatografı kullanılarak yapıldı. Bileşiklerin belirlenmesi ve tanımlanması için Mass Hunter yazılımı (Qualitative Analysis B.07.00) ve NIST Mass Spectral Library (2014) kullanıldı.
Sonuç ve Tartışma: LC-MS Q-TOF analizi, S. ruscifolia'nın rutin, narsissin, luteolin, izoramnetin, ramnetin ve kersetin dimetil eter içerdiğini gösterdi. GC-MS analizi, ekstrenin şeker (%50.5) ve şeker alkolleri (%46.39) olarak en yüksek içeriğe sahip olduğunu göstermiştir. Ayrıca karboksilik asit (%0.47), yağ asidi (%0.64), şeker asidi (%0.42), glikozit (%0.48), karotenoidler (%0.61) ve benzoik asit esteri (%0.49) içerir. D-pinitol, %41.14 ile ekstredeki en yüksek içeriğe sahiptir.

Keywords

GC/MS, Herba, LC-QTOF/MS, Silene ruscifolia

References

1. Bittrich, V. (1993). Caryophyllaceae. In: Flowering Plants, Dicotyledons, (pp. 206-236). Berlin, Heidelberg: Springer.
2. Cullen, J., Coode, M. (1967). Silene L. In: P.H. Davis (Ed.), Flora of Turkey and the East Aegean Islands, Vol. 2, (pp.179). Edinburgh: Edinburgh University Press.
3. Stevens, P.F. (2001). Angiosperm Phylogeny Website. Version 14, July 2017. http://www.mobot.org (Accessed 17.06.2022).
4. POWO (2022). Plants of the World Online. Facilitated by the Royal Botanic Gardens, Kew. Published on the Internet; http://www.plantsoftheworldonline.org (Accessed 17.06.2022).
5. Güner, A., Aslan, S., Ekim, T., Vural, M., Babaç, M.T. (2012). Türkiye bitkileri listesi (damarlı bitkiler). Nezahat Gökyigit Botanik Bahçesi Yayınları Flora Dizisi I.
6. Rigat, M., Bonet, M.À., Garcia, S., Garnatje, T., Valles, J. (2007). Studies on pharmaceutical ethnobotany in the high river Ter valley (Pyrenees, Catalonia, Iberian Peninsula). Journal of Ethnopharmacology, 113(2), 267-277. [CrossRef]
7. Kayani, S., Ahmad, M., Sultana, S., Shinwari, Z.K., Zafar, M., Yaseen, G., Bibi, T. (2015). Ethnobotany of medicinal plants among the communities of Alpine and Sub-alpine regions of Pakistan. Journal of Ethnopharmacology, 164, 186-202. [CrossRef]
8. Cakilcioglu, U., Khatun, S., Turkoglu, I., Hayta, S. (2011). Ethnopharmacological survey of medicinal plants in Maden (Elazig-Turkey). Journal of Ethnopharmacology, 137(1), 469-486. [CrossRef]
9. Cornara, L., La Rocca, A., Terrizzano, L., Dente, F., Mariotti, M.G. (2014). Ethnobotanical and phytomedical knowledge in the North-Western Ligurian Alps. Journal of Ethnopharmacology, 155(1), 463-484. [CrossRef]
10. Özdemir, E., Alpınar, K. (2015). An ethnobotanical survey of medicinal plants in western part of central Taurus Mountains: Aladaglar (Nigde-Turkey). Journal of Ethnopharmacology, 166, 53-65. [CrossRef]
11. Leto, C., Tuttolomondo, T., La Bella, S., Licata, M. (2013). Ethnobotanical study in the Madonie Regional Park (Central Sicily, Italy)-Medicinal use of wild shrub and herbaceous plant species. Journal of Ethnopharmacology, 146(1), 90-112. [CrossRef]
12. Rehman, K., Khan, M.A., Ullah, Z., Chaudhary, H.J. (2015). An ethno botanical perspective of traditional medicinal plants from the Khattak tribe of Chonthra Karak, Pakistan. Journal of Ethnopharmacology, 165, 251-259. [CrossRef]
13. Polat, R., Cakilcioglu, U., Satıl, F. (2013). Traditional uses of medicinal plants in Solhan (Bingöl-Turkey). Journal of Ethnopharmacology, 148(3), 951-963. [CrossRef]
14. Said, O., Khalil, K., Fulder, S., Azaizeh, H. (2002). Ethnopharmacological survey of medicinal herbs in Israel, the Golan Heights and the West Bank region. Journal of Ethnopharmacology, 83(3), 251-265. [CrossRef]
15. Mamadalieva, N.Z. (2012). Phytoecdysteroids from Silene plants: distribution, diversity and biological (antitumour, antibacterial and antioxidant) activities. Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas, 11(6), 474-497.
16. Mamadalieva, N.Z., Lafont, R., Wink, M. (2014). Diversity of secondary metabolites in the genus Silene L. (Caryophyllaceae)-Structures, distribution, and biological properties. Diversity, 6(3), 415-499. [CrossRef]
17. Gaidi, G., Miyamoto, T., Laurens, V., Lacaille-Dubois, M.A. (2002). New acylated triterpene saponins from Silene fortunei that modulate lymphocyte proliferation. Journal of Natural Products, 65(11), 1568-1572. [CrossRef]
18. Dötterl, S., Wolfe, L.M., Jürgens, A. (2005). Qualitative and quantitative analyses of flower scent in Silene latifolia. Phytochemistry, 66(2), 203-213. [CrossRef]
19. Eshmirzaeva, N.E., Khidyrova, N.K., Khodzhaeva, M., Mezhlumyan, L.G., Shakhidoyatov, K.M. (2005). Chemical composition of Silene viridiflora. Chemistry of Natural Compounds, 41(4), 451-453.
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Details

Primary Language	English
Subjects	Pharmacology and Pharmaceutical Sciences
Journal Section	Research Article
Authors	Kenan Can Tok 0000-0002-9353-8867 Muhammed Mesud Hürkul 0000-0002-9241-2496 Nazmiye Neslihan Bozkurt 0000-0002-5652-2954 Ayhan İbrahim Aysal 0000-0001-8048-8621 Şeyda Yayla 0000-0002-3678-6506
Publication Date	September 30, 2022
Submission Date	June 21, 2022
Acceptance Date	August 1, 2022
Published in Issue	Year 2022 Volume: 46 Issue: 3

Cite

APA	Tok, K. C., Hürkul, M. M., Bozkurt, N. N., Aysal, A. İ., et al. (2022). INVESTIGATION OF PHYTOCHEMICALS IN METHANOLIC HERBA EXTRACT OF SILENE RUSCIFOLIA BY LC-QTOF/MS AND GC/MS. Journal of Faculty of Pharmacy of Ankara University, 46(3), 827-838. https://doi.org/10.33483/jfpau.1133615
AMA	Tok KC, Hürkul MM, Bozkurt NN, Aysal Aİ, Yayla Ş. INVESTIGATION OF PHYTOCHEMICALS IN METHANOLIC HERBA EXTRACT OF SILENE RUSCIFOLIA BY LC-QTOF/MS AND GC/MS. J. Fac. Pharm. Ankara. September 2022;46(3):827-838. doi:10.33483/jfpau.1133615
Chicago	Tok, Kenan Can, Muhammed Mesud Hürkul, Nazmiye Neslihan Bozkurt, Ayhan İbrahim Aysal, and Şeyda Yayla. “INVESTIGATION OF PHYTOCHEMICALS IN METHANOLIC HERBA EXTRACT OF SILENE RUSCIFOLIA BY LC-QTOF/MS AND GC/MS”. Journal of Faculty of Pharmacy of Ankara University 46, no. 3 (September 2022): 827-38. https://doi.org/10.33483/jfpau.1133615.
EndNote	Tok KC, Hürkul MM, Bozkurt NN, Aysal Aİ, Yayla Ş (September 1, 2022) INVESTIGATION OF PHYTOCHEMICALS IN METHANOLIC HERBA EXTRACT OF SILENE RUSCIFOLIA BY LC-QTOF/MS AND GC/MS. Journal of Faculty of Pharmacy of Ankara University 46 3 827–838.
IEEE	K. C. Tok, M. M. Hürkul, N. N. Bozkurt, A. İ. Aysal, and Ş. Yayla, “INVESTIGATION OF PHYTOCHEMICALS IN METHANOLIC HERBA EXTRACT OF SILENE RUSCIFOLIA BY LC-QTOF/MS AND GC/MS”, J. Fac. Pharm. Ankara, vol. 46, no. 3, pp. 827–838, 2022, doi: 10.33483/jfpau.1133615.
ISNAD	Tok, Kenan Can et al. “INVESTIGATION OF PHYTOCHEMICALS IN METHANOLIC HERBA EXTRACT OF SILENE RUSCIFOLIA BY LC-QTOF/MS AND GC/MS”. Journal of Faculty of Pharmacy of Ankara University 46/3 (September 2022), 827-838. https://doi.org/10.33483/jfpau.1133615.
JAMA	Tok KC, Hürkul MM, Bozkurt NN, Aysal Aİ, Yayla Ş. INVESTIGATION OF PHYTOCHEMICALS IN METHANOLIC HERBA EXTRACT OF SILENE RUSCIFOLIA BY LC-QTOF/MS AND GC/MS. J. Fac. Pharm. Ankara. 2022;46:827–838.
MLA	Tok, Kenan Can et al. “INVESTIGATION OF PHYTOCHEMICALS IN METHANOLIC HERBA EXTRACT OF SILENE RUSCIFOLIA BY LC-QTOF/MS AND GC/MS”. Journal of Faculty of Pharmacy of Ankara University, vol. 46, no. 3, 2022, pp. 827-38, doi:10.33483/jfpau.1133615.
Vancouver	Tok KC, Hürkul MM, Bozkurt NN, Aysal Aİ, Yayla Ş. INVESTIGATION OF PHYTOCHEMICALS IN METHANOLIC HERBA EXTRACT OF SILENE RUSCIFOLIA BY LC-QTOF/MS AND GC/MS. J. Fac. Pharm. Ankara. 2022;46(3):827-38.

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The Journal of Faculty of Pharmacy of Ankara University is an international medium, an open access, peer-reviewed journal for the publication of original research reports, reviews and short communications in English or Turkish on relevant developments in pharmaceutical sciences., Proceeding of scientific meetings may be published as special issues of supplements to the journal. Additionally, it contains previous and forthcoming national and international scientific meetings with social activities in pharmaceutical areas.