MAKSİLLOFASİYAL CERRAHİ UYGULAMALARINDA KEMİK REJENERASYONU İÇİN BİFOSFONAT YÜKLÜ PLGA MİKROKÜRELERİ İÇEREN İN SİTU JEL FORMÜLASYONLARININ GELİŞTİRİLMESİ; FORMÜLASYONLAR, İN VİTRO KARAKTERİZASYON VE SALIM KİNETİK ÇALIŞMALARI

Year 2022, Volume: 46 Issue: 3, 993 - 1008, 30.09.2022

Heybet Kerem Polat , Sedat Ünal

https://doi.org/10.33483/jfpau.1149890

Cited By: 2

Abstract

Amaç: Bu çalışmada maksillofasiyal cerrahide implant bölgesinde kemik rejenerasyonunu artırmak için hazırlanan bifosfonat yüklü mikrosfer ilaç taşıyıcı sistemin in situ jel formülasyonu ile lokal olarak uygulanması amaçlanmıştır.
Gereç ve Yöntem: Kombinasyon taşıyıcı sistemini tasarlamak için bifosfonat yüklü PLGA mikroküreleri, hazırlanan in situ jell formülasyonlarına yüklenmiştir. Geliştirilen formülasyonlar için in vitro ilaç salım, pH, berraklık, sol-jel geçiş sıcaklığı ve salım kinetik çalışmaları değerlendirilmiştir.
Sonuç ve Tartışma: Üretilen formülasyonların yerinde jelleşme sıcaklıkları 33 ila 37°C arasında; pH değerleri 6 civarında ve bütün formülasyonlar 20 gauge’lik şırıngalardan uygulanabilir düzeydeydi. Preparatlar içerisinde yer alan, P407 ve kitosan miktarları arttıkça, in vitro patlama salınımını düşürürken aynı zamanda viskoziteyi yükselmiştir. Bununla birlikte, her bir in situ jel formülasyonu, 14 günlük bir süre içinde salım yapmıştır. Sonuç olarak, Bifosfonat yüklü PLGA mikroküreleri yüklü in situ jel formülasyonlarına ayrıntılı olarak değerlendirilmiş ve özellikle dental implant uygulamalarında maksillofasiyal cerrahide lokal olarak uygulanabilir bir ilaç taşıma sistemi olarak sunulmuştur.

Keywords

Bifosfonat, Kombinasyon Taşıma Sistemleri, PLGA Mikroküreler, Poloksamer, Kitosan

DEVELOPMENT OF IN SITU GEL FORMULATION CONTAINING BISPHOSPHONATE-LOADED PLGA MICROSPHERES FOR BONE REGENERATION IN MAXILLOFACIAL SURGERY APPLICATIONS; FORMULATIONS, IN VITRO CHARACTERIZATION AND RELEASE KINETIC STUDIES

Abstract

Objective: In this study, it was aimed to locally apply the bisphosphonate-loaded microsphere drug delivery system with in situ gel formulation, which was prepared to increase bone regeneration in the implant area in maxillofacial surgery.
Material and Method: In order to design the combination delivery system, bisphosphonate-loaded PLGA microspheres were embedded in the prepared in situ gel formulations. In vitro drug release, pH, clarity, sol-gel transition temperature and release kinetic studies were all assessed for the developed formulations.
Result and Discussion: The produced formulations' in situ gelation temperatures ranged from 33 to 37°C; their pH values were in the range of 6; and they were all syringeable, which is defined as the force required to expel each formulation from a syringe equipped with a 20-gauge needle. With the preparations, the amounts of P407 and chitosan increased, lowering in vitro burst release while simultaneously raising viscosity. However, each in situ gel formulation releases over a period of 14 days. Consequently, Bisphosphonate loaded PLGA microspheres embedded in situ gel formulations were elucidated in detail and presented as a locally applicable drug delivery system in maxillofacial surgery, especially in dental implant applications

Keywords

Bisphosphonate, Combination Delivery Systems, PLGA Microspheres, Poloxamer, Chitosan

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