COMPARISON OF ANTIOXIDANT AND ANTI-INFLAMMATORY EFFECTS OF SOME POLYPHENOLIC COMPOUNDS AND THEIR BIOTRANSFORMATION EXTRACTS

Year 2023, Volume: 47 Issue: 1, 220 - 227, 20.01.2023

Damla Kırcı , Betül Demirci

https://doi.org/10.33483/jfpau.1122665

Abstract

Objective: Microbial transformations are green biotechnological processes where different microorganisms or enzymes are used to produce new metabolites from defined substrates. Hesperidin, quercetin, and their derivatives have been proven in scientific research to have a variety of biological activities, such as antioxidant, antimicrobial, and anticancer activities.

Material and Method: This study performed the microbial transformation of hesperidin and quercetin utilizing 13 different microbial strains. The transformation extracts of hesperidin and quercetin were investigated in antioxidant (DPPH· and ABTS·+ methods) and anti-inflammatory effects.

Result and Discussion: The biotransformation of hesperidin was observed in 5 of 13 strains. There were Rhizopus stolonifera, Saccharomyces pararoseus, S. cerevisiae, Penicillium claviforme, and Fusarium solani while microbial transformation of quercetin was identified Aspergillus flavus and Penicillium claviforme. The results of this research show that the extracts obtained with the Aspergillus and Penicillium strains were more effective in terms of antioxidant and anti-inflammatory effects. DPPH· antioxidant activity of A. flavus extract (IC50: 22.55±0.32 µg/ml) was higher than the other biotransformation extracts. The IC50 value of R. stolonifer from the hesperidin transformation extracts is 25.93±0.36 µg/ml. The microbial transformation of hesperidin by Penicillium strain has not been researched previously. 

Keywords

Anti-inflammatory activity, antioxidant activity, hesperidin, microbial transformation, quercetin

Supporting Institution

Anadolu University

Project Number

1901S001

Thanks

This study is part of the PhD thesis of Damla KIRCI and was financially supported as a Scientific Research Project (1901S001) by Anadolu University.

BAZI POLİFENOLİK BİLEŞİKLERİN VE BİYOTRANSFORMASYON EKSTRELERİNİN ANTİOKSİDAN VE ANTİ-ENFLAMATUVAR AKTİVİTELERİNİN KARŞILAŞTIRILMASI

Abstract

Amaç: Mikrobiyal transformasyonlar belirlenen substratlardan yeni metabolitler üretmek için farklı mikroorganizmaların veya onların enzimlerinin kullanıldığı yeşil biyoteknolojik süreçlerdir. Hesperidin, kersetin ve türevlerinin antioksidan, antimikrobiyal ve antikanser özellikleri dahil olmak üzere çeşitli biyolojik aktivitelere sahip olduğu bilimsel araştırmalarda kanıtlanmıştır.

Gereç ve Yöntem: Bu çalışmada 13 farklı mikrobiyal suş kullanılarak hesperidin ve kersetin mikrobiyal transformasyonu gerçekleştirilmiştir. Hesperidin ve kersetinin mikrobiyal transformasyonundan elde edilen ekstrelerin antioksidan (DPPH· ve ABTS·+ yöntemleri) ve anti-enflamatuvar aktiviteleri araştırılmıştır.

Sonuç ve Tartışma: Hesperidin'in biyotransformasyonu 13 suşdan 5'inde gözlenmiştir. Rhizopus stolonifera, Saccharomyces pararoseus, S. cerevisiae, Penicillium claviforme ve Fusarium solani iken; Kersetinin mikrobiyal transformasyonu Aspergillus flavus ve Penicillium claviforme suşlarında gözlemlenmiştir.

Bu çalışmanın sonuçları Aspergillus ve Penicillium suşları ile yapılan çalışmada elde edilen ekstrelerin antioksidan ve anti-enflamatuvar aktiviteler açısından daha etkili olduğunu göstermektedir. A. flavus ekstresi (IC50: 22,55±0,32 µg/ml) DPPH· antioksidan aktivitesi diğer biyotransformasyon ekstrelerinden daha yüksek bulunmuştur. Hesperidin transformasyon ekstrelerinden R. stolonifer'in IC50 değeri 25,93±0,36 µg/ml'dir. Hesperidinin Penicillium suşu tarafından mikrobiyal dönüşümü daha önce çalışılmamıştır.

Project Number

1901S001

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