EVALUATION OF THE POTENTIAL ANTIBACTERIAL ACTIVITY OF BERBERIS CRATAEGINA DC.: WITH FOCUS ON QUORUM SENSING INHIBITION OF CHROMOBACTERIUM VIOLACEUM ATCC 12472

Year 2023, Volume: 47 Issue: 1, 178 - 184, 20.01.2023

Fatma Tuğçe Gürağaç Dereli , Evren Arın , Ebru Önem

https://doi.org/10.33483/jfpau.1165905

Abstract

Objective: In the present study described here, we set out to look into the quorum sensing inhibitory activity of the methanolic seed extract of Berberis crataegina DC. through quorum sensing -controlled inhibition of violacein pigment production in Chromobacterium violaceum ATCC 12472. In addition, the antibacterial activity of the extract on various Gram-negative and Gram-positive standard strains was evaluated. Also phenolic contents in the extract were detected by using HPLC analysis.
Material and Method: “The phytochemical profile of the seed extract was performed by High-Performance Liquid Chromatography technique. Antibacterial activity assays were performed on the extract using the agar well method and inhibition of the violacein pigment production was investigated spectrophotometrically.
Result and Discussion: According to antibacterial activity results Gram negative bacteria were more resistant than Gram positive bacteria and violacein pigment production was inhibited by 66% percentage. Phytochemical analysis results also showed that the major component in the extract is chlorogenic acid, with a value of 1974.91 µg/mL.

Keywords

Antibacterial, HPLC, phenolic content, quorum sensing

References

• 1. Wang, S. T., Gao, Y. F., Jin, Q., & Ji, J. (2020). Emerging antibacterial nanomedicine for enhanced antibiotic therapy. Biomaterials Science, 8(24), 6825-6839. [CrossRef]
• 2. Bouyahya, A., El Omari, N., El Menyiy, N., Guaouguaou, F., Balahbib, A., & Chamkhi, I. (2022). Anti- Quorum Sensing Agents from Natural Sources. In Antimicrobial Resistance (pp. 533-557): Springer.
• 3. Striednig, B., & Hilbi, H. (2022). Bacterial quorum sensing and phenotypic heterogeneity: how the collective shapes the individual. Trends in Microbiology, 30(4), 379-389. [CrossRef]
• 4. Nealson, K. H., Platt, T., & Hastings, J. W. (1970). Cellular control of the synthesis and activity of the bacterial luminescent system. J Bacteriol, 104(1), 313-322. [CrossRef]
• 5. Tay, S. B., & Yew, W. S. (2013). Development of Quorum-Based Anti-Virulence Therapeutics Targeting Gram-Negative Bacterial Pathogens. International Journal of Molecular Sciences, 14(8), 16570-16599. [CrossRef]
• 6. Albert, E., Albouy, P. A., Ayral, A., Basa, P., Csik, G., Nagy, N.,Horvolgyi, Z. (2015). Antibacterial properties of Ag-TiO2 composite solgel coatings. Rsc Advances, 5(73), 59070-59081. [CrossRef]
• 7. Pantanella, F., Berlutti, F., Passariello, C., Sarli, S., Morea, C., & Schippa, S. (2007). Violacein and biofilm production in Janthinobacterium lividum. J Appl Microbiol, 102(4), 992-999. [CrossRef]
• 8. de Lima, D. C., Medeiros, I. G., de Cassia Silva-Portela, R., da Silva Junior, F. C., Fassarela Agnez-Lima, L., de Souza, J. E. S., & Batistuzzo de Medeiros, S. R. (2021). Identification of plasmids from Brazilian Chromobacterium violaceum strains. Can J Microbiol, 1-10. [CrossRef]
• 9. Chelliah, R., Banan-MwineDaliri, E., & Oh, D. H. (2022). Screening of Actinobacteria for Quorum Sensing Inhibition. In Methods in Actinobacteriology (pp. 479-482). Humana, New York, NY: Springer.
• 10. Cárcamo, G., Sılva, M., Becerra, J., Urrutıa, H., Sossa., K, & Paz, C. (2014). Inhıbıtıon Of Quorum Sensıng By Drımane Lactones From Chılean Flora. Journal of the Chilean Chemical Society, 59(3), 2622-2624. [CrossRef]
• 11. Isikli, N. D., & Yilmaz, I. (2014). Some physical properties of sun-dried Berberis fruit (Berberis crataegina). J Food Sci Technol, 51(1), 104-110. [CrossRef]
• 12. Gonul, S., Bozkurt, B., Okudan, S., & Tugal-Tutkun, I. (2015). Bilateral acute iris transillumination following a fumigation therapy: a village-based traditional method for the treatment of ophthalmomyiasis. Cutan Ocul Toxicol, 34(1), 80-83. [CrossRef]
• 13. Charehsaz, M., Sipahi, H., Celep, E., Ustundag, A., Cemiloglu Ulker, O., Duydu, Y., . . . Yesilada, E. (2015). The fruit extract of Berberis crataegina DC: exerts potent antioxidant activity and protects DNA integrity. Daru, 23, 24. [CrossRef]
• 14. Gidik, B. (2021). Antioxidant, Antimicrobial Activities and Fatty Acid Compositions of Wild Berberis spp. by Different Techniques Combined with Chemometrics (PCA and HCA). Molecules, 26(24), 7448. [CrossRef]
• 15. Yesilada, E., & Kupeli, E. (2002). Berberis crataegina DC. root exhibits potent anti-inflammatory, analgesic and febrifuge effects in mice and rats. J Ethnopharmacol, 79(2), 237-248. [CrossRef]
• 16. Kaya, M., Ravikumar, P., Ilk, S., Mujtaba, M., Akyuz, L., Labidi, J.,Erkul, S.K. (2018). Production and characterization of chitosan based edible films from Berberis crataegina's fruit extract and seed oil. Innovative Food Science & Emerging Technologies, 45, 287-297. [CrossRef]
• 17. Özaydın, A.G., Arın, E., Önem, E. (2020). Türk Mutfağında Yeni Bir Fonksiyonel Gıda Olarak Siyah Sarımsak (Allium sativum L.): Fenolik Madde İçeriği ve Bakteriyel İletişim (Quorum Sensing) Üzerine Etkisi. Akademik Gıda, 18(27-35). [CrossRef]
• 18. Mandal, S. M., Dias, R. O., & Franco, O. L. (2017). Phenolic Compounds in Antimicrobial Therapy. Journal of Medicinal Food, 20(10), 1031-1038. [CrossRef]
• 19. Muñoz-Cazares, N., García-Contreras, R., Pérez-López, M., & Castillo-Juárez, I. (2017). Phenolic compounds with anti-virulence properties. In Phenolic Compounds: Biological Activity (pp.139-167): Intech.
• 20. Puupponen-Pimia, R., Nohynek, L., Meier, C., Kahkonen, M., Heinonen, M., Hopia, A., & Oksman- Caldentey, K. M. (2001). Antimicrobial properties of phenolic compounds from berries. J Appl Microbiol, 90(4), 494-507. [CrossRef]
• 21. Luís, Â., Silva, F., Sousa, S., Duarte, A. P., & Domingues, F. (2014). Antistaphylococcal and biofilm inhibitory activities of gallic, caffeic, and chlorogenic acids. Biofouling, 30(1), 69–79. [CrossRef]
• 22. Mostafa, I., Abbas, H.A, Ashour, M.L., Yasri, A., El-Shazly, A.M., Wink, M., Sobeh, M. (2020). Polyphenols from Salix tetrasperma Impair Virulence and Inhibit Quorum Sensing of Pseudomonas aeruginosa. Molecules. 25(6):1341. [CrossRef]
• 23. Fratianni, F., Nazzaro, F., Marandino, A., Fusco, M., Coppola, R., De Feo, V., & De Martino, L. (2013). Biochemical composition, antimicrobial activities,and anti-quorum-sensing activities of ethanol and ethyl acetate extracts from Hypericum connatum Lam. (Guttiferae). Journal of medicinal food, 16(5), 454–459. [CrossRef]