NEPHROPROTECTIVE EFFECT OF ALOE VERA EXTRACT WITH REGULATION OF OXIDATIVE STRESS, APOPTOSIS AND AQUAPORIN 3 EXPRESSION LEVELS IN STREPTOZOTOCIN INDUCED DIABETIC RATS

Uğur Şeker; Barış Can Güzel; Dila Şener; Saime Betül Baygeldi; Meral Yüksel; Özlem Unay Demirel; Sevda Soker

doi:10.33483/jfpau.1225760

Research Article

BibTex

RIS

Cite

NEPHROPROTECTIVE EFFECT OF ALOE VERA EXTRACT WITH REGULATION OF OXIDATIVE STRESS, APOPTOSIS AND AQUAPORIN 3 EXPRESSION LEVELS IN STREPTOZOTOCIN INDUCED DIABETIC RATS

Year 2023, Volume: 47 Issue: 2, 438 - 449, 20.05.2023

Uğur Şeker , Barış Can Güzel , Dila Şener , Saime Betül Baygeldi , Meral Yüksel , Özlem Unay Demirel , Sevda Soker

https://doi.org/10.33483/jfpau.1225760

Cited By: 1

Abstract

Objective: In this study we examined the protective activity of Aloe vera with considering anti-oxidant, anti-apoptotic properties, and the status of Aquaporin 3 (AQP3) channel protein.
Material and Method: Twenty-one adult female rats were divided into three groups (n=7); Control, Diabetes, Treatment. Control group did not expose to any application. Animals in Diabetes and Treatment were exposed to experimental diabetes with administration of streptozotocin. Rats in Treatment received 300 mg/kg Aloe vera extract daily for 14 days. Rats were sacrificed and kidney samples were used for analyses.
Result and Discussion: Analyses indicated that lowest malondialdehyde (MDA) and luminol levels in control group were increased significantly (P<0.05) in diabetic animals. Severe pathological changes observed in Diabetes group while microscopic examinations. Bax, Caspase-3 and apoptotic index (AI) were elevated significantly (P<0.05) in this group compared to Control. Oxidative stress, apoptotic protein expression levels and TUNEL Assay positive cell ratio were down-regulated in Treatment group. When AQP3 levels were measured, immunopositivity reduced significantly (P<0.05) in cortical kidney of Diabetes group which is normalized significantly in Treatment group.This study reporting anti-diabetic potency of Aloe vera extract has capability to avoid streptozotocin induced diabetic renal injury via regulating anti-apoptotic and anti-oxidant cellular signaling. Furthermore, Aloe vera consumption in diabetes might regulate AQP3 levels. Although we observed promising results, more studies are required to explore anti-diabetic, anti-hyperglycemic and nephroprotective activity of Aloe vera.

Keywords

Aloe vera, apoptosis, aquaporin 3, diabetes, kidney

Thanks

The Authors thank to Rohlat Seyrek for her valuable contributions.

References

1. Haghani, F., Arabnezhad, M.R., Mohammadi, S., Ghaffarian-Bahraman, A. (2022). Aloe vera and Streptozotocin-Induced Diabetes Mellitus. Revista Brasileira de Farmacognosia, 32, 174-187. [CrossRef]
2. Saeedi, P., Petersohn, I., Salpea, P., Malanda, B., Karuranga, S., Unwin, N., Colagiuri, S., Guariguata, L., Motala, A.A., Ogurtsova, K., Shaw, J.E., Bright, D., Williams, R., IDF Diabetes Atlas Committee. Diabetes Atlas Committee. (2019). Global and regional diabetes prevalence estimates for 2019 and projections for 2030 and 2045: Results from the International Diabetes Federation Diabetes Atlas. Diabetes Research and Clinical Practice, 157, 1-10. [CrossRef]
3. Hill-Briggs, F., Adler, N.E., Berkowitz, S.A., Chin, M.H., Gary-Webb, T.L., Navas-Acien, A., Thornton, P.L., Haire-Joshu, D. (2021). Social determinants of health and diabetes: a scientific review. Diabetes Care, 44(1), 258-279. [CrossRef]
4. Shahwan, M., Alhumaydhi, F., Ashraf, G. M., Hasan, P. M., Shamsi, A. (2022). Role of polyphenols in combating Type 2 Diabetes and insulin resistance. International Journal of Biological Macromolecules, 206, 567-579. [CrossRef]
5. Han, Y., Xu, X., Tang, C., Gao, P., Chen, X., Xiong, X., Yang, M., Yang, S., Zhu X., Yuan S., Liu, F., Xiao, L., Kanwar, Y.S., Sun, L. (2018). Reactive oxygen species promote tubular injury in diabetic nephropathy: The role of the mitochondrial ros-txnip-nlrp3 biological axis. Redox Biology, 16, 32-46. [CrossRef]
6. Al-Rasheed, N.M., Al-Rasheed, N.M., Bassiouni, Y.A., Hasan, I.H., Al-Amin, M.A., Al-Ajmi, H.N., Mahmoud, A.M. (2018). Simvastatin ameliorates diabetic nephropathy by attenuating oxidative stress and apoptosis in a rat model of streptozotocin-induced type 1 diabetes. Biomedicine & Pharmacotherapy, 105, 290-298. [CrossRef]
7. Malik, F.S., Taplin, C.E. (2014). Insulin therapy in children and adolescents with type 1 diabetes. Pediatric Drugs, 16, 141-150. [CrossRef]
8. McCrimmon, R.J., Frier, B.M. (1994). Hypoglycaemia, the most feared complication of insulin therapy. Diabetes & Metabolisme, 20(6), 503-512.
9. Richardson, T., Kerr, D. (2003). Skin-related complications of insulin therapy. American Journal of Clinical Dermatology. 4, 661-667. [CrossRef]
10. Sinha, A., Formica, C., Tsalamandris, C., Panagiotopoulos, S., Hendrich, E., DeLuise, M., Seeman, E., Jerums, G. (1996). Effects of insulin on body composition in patients with insulin‐dependent and non‐insulin‐dependent diabetes. Diabetic Medicine.13, 40-46. [CrossRef]
11. Tian, W., Teng, F., Gao, J., Gao, C., Liu, G., Zhang, Y., Yu, S., Zhang, W., Wang, Y., Xue, F. (2017). Estrogen and insulin synergistically promote type 1 endometrial cancer progression. Cancer Biology & Medicine, 18(12), 1000-1010. [CrossRef]
12. Yang, Y.X., Hennessy, S., Lewis, J.D. (2004). Insulin therapy and colorectal cancer risk among type 2 diabetes mellitus patients. Gastroenterology, 127(4), 1044-1050. [CrossRef]
13. Ozkol, H., Tuluce, Y., Dilsiz, N., Koyuncu, I. (2013). Therapeutic potential of some plant extracts used in Turkish traditional medicine on streptozocin-induced type 1 diabetes mellitus in rats. The Journal of Membrane Biology, 246, 47-55. [CrossRef]
14. Ajilore, B.S., Adesokan, A.A. (2018). Antidiabetic effects of Tetracarpidiumconophorum seed on biomarkers of diabetes-induced nephropathy in rats. Asian Pacific Journal of Tropical Biomedicine, 8(12), 593-597. [CrossRef]
15. Bouhrim, M., Ouassou, H., Loukili, E.H., Ramdani, M., Mekhfi, H., Ziyyat, A., Legssyer, A., Aziz, M., Bnouham, M. (2019). Antidiabetic effect of Opuntiadillenii seed oil on streptozotocin-induced diabetic rats. Asian Pacific Journal of Tropical Biomedicine, 9(9), 381-388. [CrossRef]
16. Kurt, S., Koca, R., Hürkul, M., Seker, U., Köroğlu, A. (2021). The antioxidant effect of Michauxiacampanuloides on rat ovaries. Journal of the Hellenic Veterinary Medical Society, 72(3), 3163-3170. [CrossRef]
17. Chandirasegaran, G., Elanchezhiyan, C., Ghosh, K., Sethupathy, S. (2017). Berberine chloride ameliorates oxidative stress, inflammation and apoptosis in the pancreas of Streptozotocin induced diabetic rats. Biomedicine & Pharmacotherapy, 95, 175-185. [CrossRef]
18. Hęś, M., Dziedzic, K., Górecka, D., Jędrusek-Golińska, A., Gujska, E. (2019). Aloe vera (L.) Webb.: natural sources of antioxidants-a review. Plant Foods for Human Nutrition, 74, 255-265. [CrossRef]
19. Kumar, S., Yadav, M., Yadav, A., Yadav, J. (2017). Impact of spatial and climatic conditions on phytochemical diversity and in vitro antioxidant activity of Indian Aloe vera (L.) Burm. f. South African Journal of Botany, 111, 50-59. [CrossRef]
20. Noor, A., Gunasekaran, S., Manickam, A.S., Vijayalakshmi, M.A. (2008). Antidiabetic activity of Aloe vera and histology of organs in streptozotocin-induced diabetic rats. Current Science, 94, 1070-1076.
21. Rajasekaran, S., Sivagnanam, K., Ravi, K., Subramanian, S. (2004). Hypoglycemic effect of Aloe vera gel on streptozotocin-induced diabetes in experimental rats. Journal of Medicinal Food, 7(1), 61-66. [CrossRef]
22. Şener, T.E., Yüksel, M., Özyılmaz-Yay, N., Ercan, F., Akbal, C., Şimşek, F., Şener, G. (2015). Apocynin attenuates testicular ischemia–reperfusion injury in rats. Journal of Pediatric Surgery, 50(8), 1382-1387. [CrossRef]
23. Seker, U., Nergiz, Y., Aktas, A., Akkus, M., Ozmen, M., Uyar, E., Soker, S. (2020). Trolox is more successful than allopurinol to reduce degenerative effects of testicular ischemia/reperfusion injury in rats. Journal of Pediatric Urology, 16(4), 465.E1-465.E8. [CrossRef]
24. Seker, U., Aktas, A., Nergiz, Y., Zincircioglu, S., Ketani, M. (2020). Investigation of the protective effects of melatonin, amifostine (WR-2721), and N-acetylcysteine on radiotherapy-induced uterine tissue injury in rats. International Journal of Radiation Research, 18(4), 791-798. [CrossRef]
25. DeFronzo, R.A., Reeves, W.B., Awad, A.S. (2021). Pathophysiology of diabetic kidney disease: impact of SGLT2 inhibitors. Nature Reviews Nephrology, 17, 319-334. [CrossRef]
26. Choudhury, H., Pandey, M., Hua, C.K., Mun, C.S., Jing, J.K., Kong, Ern, L.Y., Ashraf, N.A., Kit, S.W., Yee, T.S., Pichika, M.R., Gorain, B., Kesharwani, P. (2018). An update on natural compounds in the remedy of diabetes mellitus: A systematic review. Journal of Traditional and Complementary Medicine, 8(3), 361-376. [CrossRef]
27. Rehman, K., Akash, M.S.H. (2017). Mechanism of generation of oxidative stress and pathophysiology of type 2 diabetes mellitus: how are they interlinked? Journal of Cellular Biochemistry, 118, 3577-3585. [CrossRef]
28. Ren, H., Shao, Y., Wu, C., Ma, X., Lv, C., Wang, Q. (2020). Metformin alleviates oxidative stress and enhances autophagy in diabetic kidney disease via AMPK/SIRT1-FoxO1 pathway. Molecular and Cellular Endocrinology, 500, 110628. [CrossRef]
29. Yaribeygi, H., Atkin, S.L., Sahebkar, A. (2019). A review of the molecular mechanisms of hyperglycemia‐induced free radical generation leading to oxidative stress. Journal of Cellular Physiology, 234, 1300-1312. [CrossRef]
30. Zhang, P., Li, T., Wu, X., Nice, E.C., Huang, C., Zhang, Y. (2020). Oxidative stress and diabetes: antioxidative strategies. Frontiers of Medicine, 14, 583-600. [CrossRef]
31. Yagi, A., Hegazy, S., Kabbash, A., Abd-El Wahab, E. (2009). Possible hypoglycemic effect of Aloe vera L. high molecular weight fractions on type 2 diabetic patients. Saudi Pharmaceutical Journal, 17(3), 209-215. [CrossRef]
32. Rajasekaran, S., Ravi, K., Sivagnanam, K., Subramanian, S. (2006). Beneficial effects of Aloe vera leaf gel extract on lipid profile status in rats with streptozotocin diabetes. Clinical and Experimental Pharmacology and Physiology, 33, 232-237. [CrossRef]
33. Kumar, R., Sharma, B., Tomar, N.R., Roy, P., Gupta, A.K., Kumar, A. (2011). In vivo evalution of hypoglycemic activity of Aloe spp. and identification of its mode of Action on GLUT-4 gene expression in vitro. Applied Biochemistry and Biotechnology, 164, 1246-1256. [CrossRef]
34. Gao, Y., Kuok, K.I., Jin, Y., Wang, R. (2019). Biomedical applications of Aloe vera. Critical Reviews in Food Science and Nutrition, 59(supp 1), S244-S256. [CrossRef]
35. Ramachandraiahgari, R.M.Y., Somesula, S.R., Adi, P.J., Mannur, I.S., Enamala, M., Matcha, B. (2012). Protective role of ethanolic extract of Aloe vera antioxidant properties on liver and kidney of streptozotocin-induced diabetic rats. Digest Journal of Nanomaterials and Biostructures, 7(1), 175-184.
36. Kim, S., Kang, S.W., Joo, J., Han, S.H., Shin, H., Nam, B.Y., Park, J., Yoo, T.H., Kim, G., Leen, P., Park, J.T. (2021). Characterization of ferroptosis in kidney tubular cell death under diabetic conditions. Cell Death & Disease, 12, 160. [CrossRef]
37. Kumar, D., Zimpelmann, J., Robertson, S., Burns, K.D. (2004). Tubular and interstitial cell apoptosis in the streptozotocin-diabetic rat kidney. Nephron Experimental Nephrology, 96, e77-e88. [CrossRef]
38. Sohn, E., Kim, J., Kim, C.S., Kim, Y.S., Jang, D.S., Kim, J.S. (2010). Extract of the aerial parts of Aster koraiensis reduced development of diabetic nephropathy via anti-apoptosis of podocytes in streptozotocin-induced diabetic rats. Biochemical and Biophysical Research Communications, 391(1), 733-738. [CrossRef]
39. He, J., Yang, B. (2019). Aquaporins in renal diseases. International Journal of Molecular Sciences, 20(2), 366. [CrossRef]
40. Hara-Chikuma, M., Satooka, H., Watanabe, S., Honda, T., Miyachi, Y., Watanabe, T., Verkman, A.S. (2015). Aquaporin-3-mediated hydrogen peroxide transport is required for NF-κBsignalling in keratinocytes and development of psoriasis. Nature Communications, 6, 1-14. [CrossRef]
41. Ma, T., Song, Y., Yang, B., Gillespie, A., Carlson, E.J., Epstein, C.J., Verkman, A. (2000). Nephrogenic diabetes insipidus in mice lacking aquaporin-3 water channels. Proceedings of the National Academy of Sciences, 97(8), 4386-4391. [CrossRef]
42. Loh, S.Y., Giribabu, N., Gholami, K., Salleh, N. (2017). Effects of testosterone on mean arterial pressure and aquaporin (AQP)-1, 2, 3, 4, 6 and 7 expressions in the kidney of orchidectomized, adult male Sprague-Dawley rats. Archives of Biochemistry and Biophysics, 614, 41-49. [CrossRef]
43. Leung, J.C., Chan, L.Y., Tsang, A.W., Tang, S.C., Lai, K.N. (2005). Differential expression of aquaporins in the kidneys of streptozotocin‐induced diabetic mice. Nephrology, 10, 63-72. [CrossRef]
44. Tang, S., Leung, J.C., Lam, C.W., Lai, F.M.M., Chan, T.M., Lai, K.N. (2001). In vitro studies of aquaporins 1 and 3 expression in cultured human proximal tubular cells: Upregulation by transferrin but not albumin. American Journal of Kidney Diseases, 38(2), 317-330. [CrossRef]
45. Nejsum, L.N., Kwon, T.H., Marples, D., Flyvbjerg, A., Knepper, M.A., Frøkiær, J., Nielsen, S. (2001). Compensatory increase in AQP2, p-AQP2, and AQP3 expression in rats with diabetes mellitus. American Journal of Physiology-Renal Physiology, 280(4), F715-F726. [CrossRef]

ALOE VERA EKSTRAKTININ STREPTOZOTOSİN İNDÜKLÜ DİYABETIK RATLARDA OKSİDATİF STRESS, APOPTOZİS VE AQUAPORİN 3 EKSPRESYON DÜZEYİNİ DÜZENLEYEREK NEFROPROTEKTİF ETKİSİ

Year 2023, Volume: 47 Issue: 2, 438 - 449, 20.05.2023

Uğur Şeker , Barış Can Güzel , Dila Şener , Saime Betül Baygeldi , Meral Yüksel , Özlem Unay Demirel , Sevda Soker

https://doi.org/10.33483/jfpau.1225760

Cited By: 1

Abstract

Amaç: Bu çalışmada Aloe vera'nın koruyucu aktivitesini antioksidan, anti-apoptotik özellikleri ve Aquaporin 3 (AQP3) kanal proteininin durumu dikkate alınarak inceledik.
Gereç ve Yöntem: Yirmi bir yetişkin dişi sıçan Kontrol, Diyabet, Tedavi olmak üzere üç gruba (n=7) ayrıldı. Kontrol grubu herhangi bir uygulamaya maruz bırakılmadı. Diyabet ve Tedavideki hayvanlar, streptozotosin uygulanarak deneysel diyabete maruz bırakıldı. Tedavideki sıçanlara 14 gün boyunca günde 300 mg/kg Aloe vera özütü verildi. Sıçanlar sakrifiye edildi ve analizler için böbrek örnekleri kullanıldı.
Sonuç ve Tartışma: Analizler control grubundaki en düşük malondialdehit (MDA) ve luminal düzeylerinin diyabetik hayvanlarda anlamlı olarak arttığını (P<0.05) gösterdi. Diyabet grubunda mikroskobik incelemelerde ciddi patolojik değişiklikler gözlendi. Bax, Kaspaz-3 ve apoptotic indeks (AI) bu grupta Kontrol'e gore anlamlı olarak yüksek bulundu (P<0.05). Tedavi grubunda oksidatifstres, apoptotik protein ekspresyon seviyeleri ve TUNEL Assay pozitif hücre oranı aşağı regüle oldui. AQP3 düzeyleri ölçüldüğünde, Tedavi grubund aanlamlı olarak normalize edilen immünopozitiflik, Diyabet grubunun kortikal böbreğinde önemli ölçüde azaldı (P<0.05). Bu, Aloe veraekstraktının anti-diyabetik gücünü bildiren, anti-apoptotik ve antioksidan hücresel sinyallemeyi düzenleyerek streptozotosin kaynaklı diyabetik böbrek hasarını önleme yeteneğine sahip olduğunu göstermektedir. Ayrıca, diyabette Aloe vera tüketimi AQP3 seviyelerini düzenleyebilir umut verici sonuçlar gözlemlememize rağmen, Aloe vera'nın anti-diyabetik, anti-hiperglisemik ve nefroprotektif aktivitesini keşfetmek için daha fazla çalışmaya ihtiyaç vardır.

Keywords

Aloe vera, apoptoz, aquaporin 3, böbrek, diyabet

References

1. Haghani, F., Arabnezhad, M.R., Mohammadi, S., Ghaffarian-Bahraman, A. (2022). Aloe vera and Streptozotocin-Induced Diabetes Mellitus. Revista Brasileira de Farmacognosia, 32, 174-187. [CrossRef]
2. Saeedi, P., Petersohn, I., Salpea, P., Malanda, B., Karuranga, S., Unwin, N., Colagiuri, S., Guariguata, L., Motala, A.A., Ogurtsova, K., Shaw, J.E., Bright, D., Williams, R., IDF Diabetes Atlas Committee. Diabetes Atlas Committee. (2019). Global and regional diabetes prevalence estimates for 2019 and projections for 2030 and 2045: Results from the International Diabetes Federation Diabetes Atlas. Diabetes Research and Clinical Practice, 157, 1-10. [CrossRef]
3. Hill-Briggs, F., Adler, N.E., Berkowitz, S.A., Chin, M.H., Gary-Webb, T.L., Navas-Acien, A., Thornton, P.L., Haire-Joshu, D. (2021). Social determinants of health and diabetes: a scientific review. Diabetes Care, 44(1), 258-279. [CrossRef]
4. Shahwan, M., Alhumaydhi, F., Ashraf, G. M., Hasan, P. M., Shamsi, A. (2022). Role of polyphenols in combating Type 2 Diabetes and insulin resistance. International Journal of Biological Macromolecules, 206, 567-579. [CrossRef]
5. Han, Y., Xu, X., Tang, C., Gao, P., Chen, X., Xiong, X., Yang, M., Yang, S., Zhu X., Yuan S., Liu, F., Xiao, L., Kanwar, Y.S., Sun, L. (2018). Reactive oxygen species promote tubular injury in diabetic nephropathy: The role of the mitochondrial ros-txnip-nlrp3 biological axis. Redox Biology, 16, 32-46. [CrossRef]
6. Al-Rasheed, N.M., Al-Rasheed, N.M., Bassiouni, Y.A., Hasan, I.H., Al-Amin, M.A., Al-Ajmi, H.N., Mahmoud, A.M. (2018). Simvastatin ameliorates diabetic nephropathy by attenuating oxidative stress and apoptosis in a rat model of streptozotocin-induced type 1 diabetes. Biomedicine & Pharmacotherapy, 105, 290-298. [CrossRef]
7. Malik, F.S., Taplin, C.E. (2014). Insulin therapy in children and adolescents with type 1 diabetes. Pediatric Drugs, 16, 141-150. [CrossRef]
8. McCrimmon, R.J., Frier, B.M. (1994). Hypoglycaemia, the most feared complication of insulin therapy. Diabetes & Metabolisme, 20(6), 503-512.
9. Richardson, T., Kerr, D. (2003). Skin-related complications of insulin therapy. American Journal of Clinical Dermatology. 4, 661-667. [CrossRef]
10. Sinha, A., Formica, C., Tsalamandris, C., Panagiotopoulos, S., Hendrich, E., DeLuise, M., Seeman, E., Jerums, G. (1996). Effects of insulin on body composition in patients with insulin‐dependent and non‐insulin‐dependent diabetes. Diabetic Medicine.13, 40-46. [CrossRef]
11. Tian, W., Teng, F., Gao, J., Gao, C., Liu, G., Zhang, Y., Yu, S., Zhang, W., Wang, Y., Xue, F. (2017). Estrogen and insulin synergistically promote type 1 endometrial cancer progression. Cancer Biology & Medicine, 18(12), 1000-1010. [CrossRef]
12. Yang, Y.X., Hennessy, S., Lewis, J.D. (2004). Insulin therapy and colorectal cancer risk among type 2 diabetes mellitus patients. Gastroenterology, 127(4), 1044-1050. [CrossRef]
13. Ozkol, H., Tuluce, Y., Dilsiz, N., Koyuncu, I. (2013). Therapeutic potential of some plant extracts used in Turkish traditional medicine on streptozocin-induced type 1 diabetes mellitus in rats. The Journal of Membrane Biology, 246, 47-55. [CrossRef]
14. Ajilore, B.S., Adesokan, A.A. (2018). Antidiabetic effects of Tetracarpidiumconophorum seed on biomarkers of diabetes-induced nephropathy in rats. Asian Pacific Journal of Tropical Biomedicine, 8(12), 593-597. [CrossRef]
15. Bouhrim, M., Ouassou, H., Loukili, E.H., Ramdani, M., Mekhfi, H., Ziyyat, A., Legssyer, A., Aziz, M., Bnouham, M. (2019). Antidiabetic effect of Opuntiadillenii seed oil on streptozotocin-induced diabetic rats. Asian Pacific Journal of Tropical Biomedicine, 9(9), 381-388. [CrossRef]
16. Kurt, S., Koca, R., Hürkul, M., Seker, U., Köroğlu, A. (2021). The antioxidant effect of Michauxiacampanuloides on rat ovaries. Journal of the Hellenic Veterinary Medical Society, 72(3), 3163-3170. [CrossRef]
17. Chandirasegaran, G., Elanchezhiyan, C., Ghosh, K., Sethupathy, S. (2017). Berberine chloride ameliorates oxidative stress, inflammation and apoptosis in the pancreas of Streptozotocin induced diabetic rats. Biomedicine & Pharmacotherapy, 95, 175-185. [CrossRef]
18. Hęś, M., Dziedzic, K., Górecka, D., Jędrusek-Golińska, A., Gujska, E. (2019). Aloe vera (L.) Webb.: natural sources of antioxidants-a review. Plant Foods for Human Nutrition, 74, 255-265. [CrossRef]
19. Kumar, S., Yadav, M., Yadav, A., Yadav, J. (2017). Impact of spatial and climatic conditions on phytochemical diversity and in vitro antioxidant activity of Indian Aloe vera (L.) Burm. f. South African Journal of Botany, 111, 50-59. [CrossRef]
20. Noor, A., Gunasekaran, S., Manickam, A.S., Vijayalakshmi, M.A. (2008). Antidiabetic activity of Aloe vera and histology of organs in streptozotocin-induced diabetic rats. Current Science, 94, 1070-1076.
21. Rajasekaran, S., Sivagnanam, K., Ravi, K., Subramanian, S. (2004). Hypoglycemic effect of Aloe vera gel on streptozotocin-induced diabetes in experimental rats. Journal of Medicinal Food, 7(1), 61-66. [CrossRef]
22. Şener, T.E., Yüksel, M., Özyılmaz-Yay, N., Ercan, F., Akbal, C., Şimşek, F., Şener, G. (2015). Apocynin attenuates testicular ischemia–reperfusion injury in rats. Journal of Pediatric Surgery, 50(8), 1382-1387. [CrossRef]
23. Seker, U., Nergiz, Y., Aktas, A., Akkus, M., Ozmen, M., Uyar, E., Soker, S. (2020). Trolox is more successful than allopurinol to reduce degenerative effects of testicular ischemia/reperfusion injury in rats. Journal of Pediatric Urology, 16(4), 465.E1-465.E8. [CrossRef]
24. Seker, U., Aktas, A., Nergiz, Y., Zincircioglu, S., Ketani, M. (2020). Investigation of the protective effects of melatonin, amifostine (WR-2721), and N-acetylcysteine on radiotherapy-induced uterine tissue injury in rats. International Journal of Radiation Research, 18(4), 791-798. [CrossRef]
25. DeFronzo, R.A., Reeves, W.B., Awad, A.S. (2021). Pathophysiology of diabetic kidney disease: impact of SGLT2 inhibitors. Nature Reviews Nephrology, 17, 319-334. [CrossRef]
26. Choudhury, H., Pandey, M., Hua, C.K., Mun, C.S., Jing, J.K., Kong, Ern, L.Y., Ashraf, N.A., Kit, S.W., Yee, T.S., Pichika, M.R., Gorain, B., Kesharwani, P. (2018). An update on natural compounds in the remedy of diabetes mellitus: A systematic review. Journal of Traditional and Complementary Medicine, 8(3), 361-376. [CrossRef]
27. Rehman, K., Akash, M.S.H. (2017). Mechanism of generation of oxidative stress and pathophysiology of type 2 diabetes mellitus: how are they interlinked? Journal of Cellular Biochemistry, 118, 3577-3585. [CrossRef]
28. Ren, H., Shao, Y., Wu, C., Ma, X., Lv, C., Wang, Q. (2020). Metformin alleviates oxidative stress and enhances autophagy in diabetic kidney disease via AMPK/SIRT1-FoxO1 pathway. Molecular and Cellular Endocrinology, 500, 110628. [CrossRef]
29. Yaribeygi, H., Atkin, S.L., Sahebkar, A. (2019). A review of the molecular mechanisms of hyperglycemia‐induced free radical generation leading to oxidative stress. Journal of Cellular Physiology, 234, 1300-1312. [CrossRef]
30. Zhang, P., Li, T., Wu, X., Nice, E.C., Huang, C., Zhang, Y. (2020). Oxidative stress and diabetes: antioxidative strategies. Frontiers of Medicine, 14, 583-600. [CrossRef]
31. Yagi, A., Hegazy, S., Kabbash, A., Abd-El Wahab, E. (2009). Possible hypoglycemic effect of Aloe vera L. high molecular weight fractions on type 2 diabetic patients. Saudi Pharmaceutical Journal, 17(3), 209-215. [CrossRef]
32. Rajasekaran, S., Ravi, K., Sivagnanam, K., Subramanian, S. (2006). Beneficial effects of Aloe vera leaf gel extract on lipid profile status in rats with streptozotocin diabetes. Clinical and Experimental Pharmacology and Physiology, 33, 232-237. [CrossRef]
33. Kumar, R., Sharma, B., Tomar, N.R., Roy, P., Gupta, A.K., Kumar, A. (2011). In vivo evalution of hypoglycemic activity of Aloe spp. and identification of its mode of Action on GLUT-4 gene expression in vitro. Applied Biochemistry and Biotechnology, 164, 1246-1256. [CrossRef]
34. Gao, Y., Kuok, K.I., Jin, Y., Wang, R. (2019). Biomedical applications of Aloe vera. Critical Reviews in Food Science and Nutrition, 59(supp 1), S244-S256. [CrossRef]
35. Ramachandraiahgari, R.M.Y., Somesula, S.R., Adi, P.J., Mannur, I.S., Enamala, M., Matcha, B. (2012). Protective role of ethanolic extract of Aloe vera antioxidant properties on liver and kidney of streptozotocin-induced diabetic rats. Digest Journal of Nanomaterials and Biostructures, 7(1), 175-184.
36. Kim, S., Kang, S.W., Joo, J., Han, S.H., Shin, H., Nam, B.Y., Park, J., Yoo, T.H., Kim, G., Leen, P., Park, J.T. (2021). Characterization of ferroptosis in kidney tubular cell death under diabetic conditions. Cell Death & Disease, 12, 160. [CrossRef]
37. Kumar, D., Zimpelmann, J., Robertson, S., Burns, K.D. (2004). Tubular and interstitial cell apoptosis in the streptozotocin-diabetic rat kidney. Nephron Experimental Nephrology, 96, e77-e88. [CrossRef]
38. Sohn, E., Kim, J., Kim, C.S., Kim, Y.S., Jang, D.S., Kim, J.S. (2010). Extract of the aerial parts of Aster koraiensis reduced development of diabetic nephropathy via anti-apoptosis of podocytes in streptozotocin-induced diabetic rats. Biochemical and Biophysical Research Communications, 391(1), 733-738. [CrossRef]
39. He, J., Yang, B. (2019). Aquaporins in renal diseases. International Journal of Molecular Sciences, 20(2), 366. [CrossRef]
40. Hara-Chikuma, M., Satooka, H., Watanabe, S., Honda, T., Miyachi, Y., Watanabe, T., Verkman, A.S. (2015). Aquaporin-3-mediated hydrogen peroxide transport is required for NF-κBsignalling in keratinocytes and development of psoriasis. Nature Communications, 6, 1-14. [CrossRef]
41. Ma, T., Song, Y., Yang, B., Gillespie, A., Carlson, E.J., Epstein, C.J., Verkman, A. (2000). Nephrogenic diabetes insipidus in mice lacking aquaporin-3 water channels. Proceedings of the National Academy of Sciences, 97(8), 4386-4391. [CrossRef]
42. Loh, S.Y., Giribabu, N., Gholami, K., Salleh, N. (2017). Effects of testosterone on mean arterial pressure and aquaporin (AQP)-1, 2, 3, 4, 6 and 7 expressions in the kidney of orchidectomized, adult male Sprague-Dawley rats. Archives of Biochemistry and Biophysics, 614, 41-49. [CrossRef]
43. Leung, J.C., Chan, L.Y., Tsang, A.W., Tang, S.C., Lai, K.N. (2005). Differential expression of aquaporins in the kidneys of streptozotocin‐induced diabetic mice. Nephrology, 10, 63-72. [CrossRef]
44. Tang, S., Leung, J.C., Lam, C.W., Lai, F.M.M., Chan, T.M., Lai, K.N. (2001). In vitro studies of aquaporins 1 and 3 expression in cultured human proximal tubular cells: Upregulation by transferrin but not albumin. American Journal of Kidney Diseases, 38(2), 317-330. [CrossRef]
45. Nejsum, L.N., Kwon, T.H., Marples, D., Flyvbjerg, A., Knepper, M.A., Frøkiær, J., Nielsen, S. (2001). Compensatory increase in AQP2, p-AQP2, and AQP3 expression in rats with diabetes mellitus. American Journal of Physiology-Renal Physiology, 280(4), F715-F726. [CrossRef]

There are 45 citations in total.

Details

Primary Language	English
Subjects	Pharmacology and Pharmaceutical Sciences
Journal Section	Research Article
Authors	Uğur Şeker 0000-0002-1693-6378 Barış Can Güzel 0000-0002-2504-120X Dila Şener 0000-0001-9496-7324 Saime Betül Baygeldi 0000-0002-4403-8663 Meral Yüksel 0000-0002-4760-3306 Özlem Unay Demirel 0000-0002-3059-9398 Sevda Soker 0000-0001-6453-0033
Early Pub Date	May 17, 2023
Publication Date	May 20, 2023
Submission Date	December 28, 2022
Acceptance Date	February 14, 2023
Published in Issue	Year 2023 Volume: 47 Issue: 2

Cite

APA	Şeker, U., Güzel, B. C., Şener, D., Baygeldi, S. B., et al. (2023). NEPHROPROTECTIVE EFFECT OF ALOE VERA EXTRACT WITH REGULATION OF OXIDATIVE STRESS, APOPTOSIS AND AQUAPORIN 3 EXPRESSION LEVELS IN STREPTOZOTOCIN INDUCED DIABETIC RATS. Journal of Faculty of Pharmacy of Ankara University, 47(2), 438-449. https://doi.org/10.33483/jfpau.1225760
AMA	Şeker U, Güzel BC, Şener D, Baygeldi SB, Yüksel M, Unay Demirel Ö, Soker S. NEPHROPROTECTIVE EFFECT OF ALOE VERA EXTRACT WITH REGULATION OF OXIDATIVE STRESS, APOPTOSIS AND AQUAPORIN 3 EXPRESSION LEVELS IN STREPTOZOTOCIN INDUCED DIABETIC RATS. J. Fac. Pharm. Ankara. May 2023;47(2):438-449. doi:10.33483/jfpau.1225760
Chicago	Şeker, Uğur, Barış Can Güzel, Dila Şener, Saime Betül Baygeldi, Meral Yüksel, Özlem Unay Demirel, and Sevda Soker. “NEPHROPROTECTIVE EFFECT OF ALOE VERA EXTRACT WITH REGULATION OF OXIDATIVE STRESS, APOPTOSIS AND AQUAPORIN 3 EXPRESSION LEVELS IN STREPTOZOTOCIN INDUCED DIABETIC RATS”. Journal of Faculty of Pharmacy of Ankara University 47, no. 2 (May 2023): 438-49. https://doi.org/10.33483/jfpau.1225760.
EndNote	Şeker U, Güzel BC, Şener D, Baygeldi SB, Yüksel M, Unay Demirel Ö, Soker S (May 1, 2023) NEPHROPROTECTIVE EFFECT OF ALOE VERA EXTRACT WITH REGULATION OF OXIDATIVE STRESS, APOPTOSIS AND AQUAPORIN 3 EXPRESSION LEVELS IN STREPTOZOTOCIN INDUCED DIABETIC RATS. Journal of Faculty of Pharmacy of Ankara University 47 2 438–449.
IEEE	U. Şeker, B. C. Güzel, D. Şener, S. B. Baygeldi, M. Yüksel, Ö. Unay Demirel, and S. Soker, “NEPHROPROTECTIVE EFFECT OF ALOE VERA EXTRACT WITH REGULATION OF OXIDATIVE STRESS, APOPTOSIS AND AQUAPORIN 3 EXPRESSION LEVELS IN STREPTOZOTOCIN INDUCED DIABETIC RATS”, J. Fac. Pharm. Ankara, vol. 47, no. 2, pp. 438–449, 2023, doi: 10.33483/jfpau.1225760.
ISNAD	Şeker, Uğur et al. “NEPHROPROTECTIVE EFFECT OF ALOE VERA EXTRACT WITH REGULATION OF OXIDATIVE STRESS, APOPTOSIS AND AQUAPORIN 3 EXPRESSION LEVELS IN STREPTOZOTOCIN INDUCED DIABETIC RATS”. Journal of Faculty of Pharmacy of Ankara University 47/2 (May 2023), 438-449. https://doi.org/10.33483/jfpau.1225760.
JAMA	Şeker U, Güzel BC, Şener D, Baygeldi SB, Yüksel M, Unay Demirel Ö, Soker S. NEPHROPROTECTIVE EFFECT OF ALOE VERA EXTRACT WITH REGULATION OF OXIDATIVE STRESS, APOPTOSIS AND AQUAPORIN 3 EXPRESSION LEVELS IN STREPTOZOTOCIN INDUCED DIABETIC RATS. J. Fac. Pharm. Ankara. 2023;47:438–449.
MLA	Şeker, Uğur et al. “NEPHROPROTECTIVE EFFECT OF ALOE VERA EXTRACT WITH REGULATION OF OXIDATIVE STRESS, APOPTOSIS AND AQUAPORIN 3 EXPRESSION LEVELS IN STREPTOZOTOCIN INDUCED DIABETIC RATS”. Journal of Faculty of Pharmacy of Ankara University, vol. 47, no. 2, 2023, pp. 438-49, doi:10.33483/jfpau.1225760.
Vancouver	Şeker U, Güzel BC, Şener D, Baygeldi SB, Yüksel M, Unay Demirel Ö, Soker S. NEPHROPROTECTIVE EFFECT OF ALOE VERA EXTRACT WITH REGULATION OF OXIDATIVE STRESS, APOPTOSIS AND AQUAPORIN 3 EXPRESSION LEVELS IN STREPTOZOTOCIN INDUCED DIABETIC RATS. J. Fac. Pharm. Ankara. 2023;47(2):438-49.

Cited By

Macroscopic and Microscopic Development of the Stomach in Hamdani Crossbred Sheep Fetuses (Ovis aries)

Journal of Research in Veterinary Medicine

https://doi.org/10.30782/jrvm.1477905

Download Cover Image

Article Files

Full Text

Scope and Aim

The Journal of Faculty of Pharmacy of Ankara University is an international medium, an open access, peer-reviewed journal for the publication of original research reports, reviews and short communications in English or Turkish on relevant developments in pharmaceutical sciences., Proceeding of scientific meetings may be published as special issues of supplements to the journal. Additionally, it contains previous and forthcoming national and international scientific meetings with social activities in pharmaceutical areas.