CANLI DONÖR KARACİĞER NAKLİNİN ÖNGÖRÜSEL BİYOBELİRTEÇLERİ

Year 2023, Volume: 47 Issue: 3, 957 - 966, 20.09.2023

Ömer Faruk Çiftçi , Tevfik Tolga Şahin , Hande Yüce , Sezai Yılmaz , Neşe Başak Türkmen , Şeyma Yaşar , Tülay Çoban , Songül Ünüvar

https://doi.org/10.33483/jfpau.1247781

Abstract

Amaç: Canlı donör karaciğer nakli (CDKN) şu anda dünya çapında en yaygın karaciğer nakli yaklaşımıdır. Rejeksiyon reaksiyonuna yol açabilecek erken nakil sonrası komplikasyonları tespit etmek için greft durumunu öngören uygun biyobelirteçler kullanılmalıdır.
Gereç ve Yöntem: Çalışmaya toplam 44 karaciğer alıcısı ve 44 karaciğer donörü dahil edildi, bunlardan ameliyat öncesi kan örnekleri toplandı, immünoassay ve spektrofotometrik analizler yapıldı. Serum neopterin, interferon gama (IFN-y), indolamin-2,3-dioksijenaz (IDO) ve alfa- Glutatyon S- transferaz (a-GST) seviyeleri, enzime bağlı bir immünosorbent deneyi kullanılarak ölçüldü. Eritrositlerdeki glutatyon redüktaz (GR) ve glukoz-6-fosfat dehidrogenaz (G6PD) aktivitesi spektrofotometri ile ölçüldü.
Sonuç ve Tartışma: Neopterin, IDO ve G6PD düzeyleri alıcı grupta donör grubuna göre anlamlı olarak yüksekti. Cinsiyet ve kan gruplarındaki farklılıklar istatistiksel olarak anlamsızdı. Hastaların %25’inde reddetme reaksiyonu gelişti ve hiçbiri hayatta kalmadı. Bu bulgular, CDKN sonrası akut rejeksiyon reaksiyonlarının teşhisi için yeni prediktif biyobelirteçlerin tanımlanmasını kolaylaştırabilir. Yeni invaziv olmayan biyobelirteçlerin klinik kullanımı, zaman ve maliyet avantajları sağlayabilir.

Keywords

α-GST karaciğer nakli, G6PD, IFN-γ, indolamin-2,3-dioksijenaz

Project Number

TDK-2019-1641

PREDICTIVE BIOMARKERS OF LIVING DONOR LIVER TRANSPLANTATION

Abstract

Objective: Liver transplantation using a living donor (LDLT) is currently the most popular method used in the worldwide. Appropriate biomarkers that predict graft status should be used to detect early post-transplant complications that may lead to a rejection reaction.
Material and Method: The study involved a total of 44 liver recipients and 44 liver donors, from whom preoperative blood samples were taken and immunoassay and spectrophotometric studies were carried out. The levels of serum neopterin, interferon-gamma (IFN- y), indoleamine-2,3 dioxygenase (IDO), and -glutathione S transferase (a-GST) were assessed using an enzyme-linked immunosorbent test. The activity of glutathione reductase (GR) and glucose-6-phosphate dehydrogenase (G6PD) in erythrocytes was measured using spectrophotometry.
Result and Discussion: “Neopterin, IDO, and G6PD levels were significantly higher in the recipient group than in the donor group. The differences in gender and blood groups were statistically insignificant. The rejection reaction developed in 25% of patients and none survived. These findings may facilitate the identification of novel predictive biomarkers for the diagnosis of acute rejection reactions after LDLT. The clinical use of novel non-invasive biomarkers may provide time and cost advantages.

Keywords

α-GST liver transplantation, G6PD, IFN-γ, indoleamine-2,3-dioxygenase

Supporting Institution

İNÖNÜ ÜNİVERSİTESİ BİLİMSEL ARAŞTIRMA PROJE BİRİMİ (İUBAP)

Project Number

TDK-2019-1641

Thanks

The Department of Scientific Research Projects at İnönü University provided funding for the present study (Project number: TDK-2019-1641).

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