ELECTROCHEMICAL INVESTIGATION OF OTILONIUM BROMIDE USING BORON-DOPED DIAMOND AND GLASSY CARBON ELECTRODES

Year 2023, Volume: 47 Issue: 3, 1003 - 1010, 20.09.2023

Leyla Karadurmuş , Esen Bellur Atici , Sibel A. Özkan

https://doi.org/10.33483/jfpau.1344014

Abstract

Objective: Using cyclic (CV) and differential pulse (DPV) voltammetric techniques, the electrochemical research of otilonium bromide (OTB) was carried out over a wide pH range (0.3–12) at glassy carbon electrodes (GCE) and boron-doped diamond electrodes (BDDE). The typical electrochemical behavior of OTB was identified as being dependent on the type of working electrode and pH. This research aims to provide a brand-new electroanalytical technique for measuring OTB in buffer solutions.
Material and Method: All experiments employed the typical three-electrode cell of 10 ml capacity in conjunction with a platinum wire counter electrode, a BDDE and GCE working electrode, and an Ag/AgCl reference electrode. NOVA 1.8 software and an AUTOLAB 204 potentiostat/galvanostat were used for electrochemical measurements.
Result and Discussion: The electrochemical behavior of OTB, which belongs to a class of drugs called 'antispasmodics' (spasm and cramps reliever), primarily used to treat irritable bowel syndrome (IBS), and other gastrointestinal conditions characterized by motility problems, painful bowel spasms and distension (swelling and bloating in the belly area), was examined in 0.1 M H2SO4 at BDDE and GCE. The electrooxidation mechanism was also investigated by conducting CV investigations at various pH levels throughout a broad pH range (pH 0.3-12.0). Understanding the mechanism was aided by scan rate investigations, which revealed that diffusion was controlled for both electrodes. The proposed technique was successfully used to determine OTB under optimal conditions.

Keywords

Boron-doped diamond electrode, differential pulse voltammetry, drug assay, glassy carbon electrode, Otilonium bromide

CAMSI KARBON VE BOR KATKILI ELMAS ELEKTROTLAR KULLANARAK OTİLONYUM BROMÜRÜN ELEKTROKİMYASAL DEĞERLENDİRİLMESİ

Abstract

Amaç: Döngüsel (CV) ve diferansiyel darbe (DPV) voltametrik teknikler kullanılarak, otilonyum bromürün (OTB) elektrokimyasal araştırması, bor katkılı elmas elektrotlarda (BDDE) ve camsı karbon elektrotlarda (GCE) geniş bir pH aralığında (0.3–12) gerçekleştirildi. OTB'nin tipik elektrokimyasal davranışının, çalışan elektrot tipine ve pH'a bağlı olduğu tespit edildi. Bu araştırmanın amacı, tampon çözeltilerde OTB'yi ölçmek için yepyeni bir elektroanalitik teknik sağlamaktır.
Gereç ve Yöntem: 10 ml kapasiteli tipik tek bölmeli üç elektrotlu hücre, tüm çalışmalarda bir BDDE ve GCE çalışma elektrotu, bir platin tel karşıt elektrot ve bir Ag/AgCl referans elektrotu ile birlikte kullanıldı. Elektrokimyasal ölçümleri gerçekleştirmek için NOVA 1.8 yazılımı ve bir AUTOLAB 204 potansiyostat/galvanostat kullanıldı.
Sonuç ve Tartışma: "Antispazmodikler" (spazm ve kramp giderici) adı verilen bir ilaç sınıfına ait olan OTB'nin elektrokimyasal davranışı, öncelikle hassas bağırsak sendromunu (IBS) ve hareketlilik sorunları, ağrılı bağırsak spazmları ve şişkinliği (şişme) ile karakterize edilen diğer gastrointestinal rahatsızlıkları tedavi etmek için kullanılır. ve göbek bölgesinde şişkinlik), GCE ve BDDE'de 0.1 M H2SO4'te incelenmiştir. Geniş bir pH aralığı (pH 0.3-12.0) boyunca çeşitli pH seviyelerinde CV araştırmaları yapılarak elektrooksidasyon mekanizması da araştırıldı. Mekanizmanın anlaşılması, difüzyonun her iki elektrot için de kontrol edildiğini ortaya koyan tarama hızı araştırmalarıyla desteklendi. Önerilen yöntem, optimal koşullar altında OTB'yi belirlemek için başarıyla kullanılmıştır.

Keywords

Bor katkılı elmas elektrot, camsı karbon elektrot, diferansiyel puls voltammetrisi, ilaç analizi, Otilonyum bromür

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