RAPHANUS SATIVUS L. ‘RED MEAT’ KÖKLERİNİN SUBKRİTİK SU VE MİKRODALGA EKSTRELERİNİN KOLİNESTERAZ VE TİROZİNAZ İNHİBİTÖR AKTİVİTESİ
Year 2024,
Volume: 48 Issue: 3, 25 - 25
Halil Koyu
Abstract
Amaç: Raphanus sativus L. Red Meat köklerinin mikrodalga destekli ve subkritik su ekstrelerinin kolinesteraz ve tirozinaz inhibitör aktivite potansiyeli araştırılmıştır.
Gereç ve Yöntem: Geleneksel çözücü ve gelişmiş ekstraksiyon sistemlerinden elde edilen ekstrelerin toplam fenol, flavonoid ve antosiyanin içeriği spektrofotometrik olarak ölçülmüştür. Asetilkolinesteraz, bütirilkolinesteraz ve tirozinaz inhibitör aktiviteleri, kinetik okumalarla absorbans değişim hızının hesaplanmasıyla araştırılmıştır.
Sonuç ve Tartışma: Subkritik su ekstresinin sırasıyla 0.71 mg/ml, 2.13 mg/ml ve 1.21 mg/ml IC50 değerleri ile en yüksek asetilkolinesteraz, bütirilkolinesteraz ve tirozinaz inhibitör aktivitelerini sağladığı bulunmuştur. Toplam fenol (27.57 mg GAE/g) ve flavonoid (4.80 mg QE/g) içeriği analizi ile de tutarlı olarak, kırmızı etli turpun subkritik su ekstresi enzim inhibitör aktivite amacıyla üretilen ürünler için potansiyel bir kaynak olarak değerlendirilebilir.
Project Number
2018-ÖNAP-ECZF-0009
References
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CHOLINESTERASE AND TYROSINASE INHIBITORY ACTIVITY OF SUBCRITICAL WATER AND MICROWAVE EXTRACTS OF RAPHANUS SATIVUS L. ‘RED MEAT’ RADIX
Year 2024,
Volume: 48 Issue: 3, 25 - 25
Halil Koyu
Abstract
Objective: Cholinesterase and tyrosinase inhibitory activity potential of microwave-assisted and subcritical water extracts of Raphanus sativus L. Red Meat roots were investigated.
Material and Method: Total phenol, flavonoid and anthocyanin content of extracts from conventional solvent and advanced extraction systems were spectrophotometrically quantified. Acetylcholinesterase, butyrylcholinesterase and tyrosinase inhibitory activities were investigated with the calculation of the rate of absorbance change with kinetic readings.
Result and Discussion: The subcritical water extract was found to provide highest acetylcholinesterase, butyrylcholinesterase and tyrosinase inhibitory activities with an IC50 of 0.71 mg/ml, 2.13 mg/ml and 1.21 mg/ml, respectively. Consistent with the anaylsis of total phenol (27.57 mg GAE/g) and flavonoid (4.80 mg QE/g) contents, subcritical water extract of red meat radish can be considered as a potential source for products aiming enzyme inhibitory activity.
Ethical Statement
The author declares that the ethics committee approval is not required for this study.
Supporting Institution
This work was a part of a project supported by Izmir Katip Celebi University Scientific Research Projects Funds [2018-ÖNAP-ECZF-0009]
Project Number
2018-ÖNAP-ECZF-0009
Thanks
Access to the facilities of Novel Fluidic Technologies Laboratory and Pharmaceutical Sciences Research Center (FABAL) at Ege University, and Central Research Laboratories (MERLAB) at Izmir Katip Celebi University are highly appreciated.
References
- 1. Kopta, T., Pokluda, R. (2013). Yields, quality and nutritional parameters of radish (Raphanus sativus) cultivars when grown organically in the Czech Republic. Horticultural Science, 40, 16-21. [CrossRef]
- 2. Lee, S. W., Yang, K.M., Kim, J.K., Nam, B.H., Lee, C.M., Jeong, M.H., Seo, S.Y., Kim, G.Y., Jo, W.S. (2012). Effects of white radish (Raphanus sativus) enzyme extract on hepatotoxicity. Toxicological Research, 28, 165-172. [CrossRef]
- 3. Gokdemir, O., Yucetepe, A. (2022). Ultrasound bath- and ultrasound probe-assisted extractions of polyphenolics from pulp and peel of red radish (Raphanus sativus L.): Investigation of changes in antioxidant activity during in vitro digestion. Gıda The Journal of Food, 47, 1046-1058. [CrossRef]
- 4. Saha, S., Paul, S., Afroz, A., Dey, A., Chatterjee, A., Khanra, R. (2023). Raphanus sativus - A review of its traditional uses, phytochemistry, and pharmacology. Asian Journal of Pharmaceutical and Clinical Research, 16, 7-12. [CrossRef]
- 5. Gao, L., Li, H., Li, B., Shao, H., Yu, X., Miao, Z., Zhang, L., Zhu, L., Sheng, H. (2022). Traditional uses, phytochemistry, transformation of ingredients and pharmacology of the dried seeds of Raphanus sativus L. (Raphani Semen), A comprehensive review. Journal of Ethnopharmacology, 294, 115387. [CrossRef]
- 6. Koyu, H., Demir, S. (2024). Subcritical water extraction of red cabbage regarding cholinesterase and tyrosinase inhibitory activity. Microchemical Journal, 199, 109953. [CrossRef]
- 7. Koyu, H., Demir, S., Haznedaroglu, M.Z. (2023). Investigation of microwave extraction of red cabbage and its neurotherapeutic potential. Journal of Food and Drug Analysis, 31. [CrossRef]
- 8. Koyu, H., Kazan, A., Ozturk, T.K., Yesil-Celiktas, O., Haznedaroglu, M.Z. (2017). Optimizing subcritical water extraction of L. fruits for maximization of tyrosinase inhibitory activity. Journal of Supercritical Fluids, 127, 15-22. [CrossRef]
- 9. Koyu, H., Kazan, A., Demir, S., Haznedaroglu, M.Z., Yesil-Celiktas, O. (2018). Optimization of microwave assisted extraction of Morus nigra L. fruits maximizing tyrosinase inhibitory activity with isolation of bioactive constituents. Food Chemistry, 248, 183-191. [CrossRef]
- 10. Chang, T.S. (2009). An updated review of tyrosinase inhibitors. International Journal of Molecular Sciences, 10, 2440-2475. [CrossRef]
- 11. Bochot, C., Favre, E., Dubois, C., Baptiste, B., Bubacco, L., Carrupt, P. A., Gellon, G., Hardre, R., Luneau, D., Moreau, Y., Nurisso, A., Reglier, M., Serratrice, G., Belle, C., Jamet, H. (2013). Unsymmetrical binding modes of the HOPNO inhibitor of tyrosinase: From model complexes to the enzyme. Chemistry-A European Journal, 19, 3655-3664. [CrossRef]
- 12. Senol, F.S., Orhan, I., Yilmaz, G., Cicek, M., Sener, B. (2010). Acetylcholinesterase, butyrylcholinesterase, and tyrosinase inhibition studies and antioxidant activities of 33 Scutellaria L. taxa from Turkey. Food and Chemical Toxicology, 48, 781-788. [CrossRef]
- 13. Scott, L.J., Goa, K.L. (2000). Galantamine - A review of its use in Alzheimer's disease. Drugs, 60, 1095-1122. [CrossRef]
- 14. Rolinski, M., Fox, C., Maidment, I., McShane, R. (2012). Cholinesterase inhibitors for dementia with Lewy bodies, Parkinson's disease dementia and cognitive impairment in Parkinson's disease. Cochrane Database of Systematic Reviews, 2012, 1-50. [CrossRef]
- 15. Çinkir, N.I., Süfer, Ö. (2020). Microwave drying of Turkish red meat (watermelon) radish (Raphanus sativus L.): effect of osmotic dehydration, pre-treatment and slice thickness. Heat and Mass Transfer, 56, 3303-3313. [CrossRef]
- 16. Alara, O.R., Abdurahman, N.H., Ukaegbu, C.I. (2021). Extraction of phenolic compounds: A review. Current Research in Food Science, 4, 200-214. [CrossRef]
- 17. Cankurtaran-Komurcu, T. (2023). Evaluation of the chemical and functional properties of powders of different radish (Raphanus sativus) cultivars as a potential functional food. Gıda The Journal of Food, 48, 1185-1198. [CrossRef]
- 18. Rodriguez-Saona, L.E., Giusti, M.M., Durst, R.W., Wrolstad, R.E. (2001). Development and process optimization of red radish concentrate extract as potential natural red colorant. Journal of Food Processing and Preservation, 25, 165-182. [CrossRef]
- 19. Matus-Castillo, D.M., Moya-Hernández, J.C., Castillo-Guevara, C., Cervantes-Rodriguez, M., Arguelles-Martinez, L., Aguilar- Paredes, O.A., Méndez-Iturbide, D. (2022). Extraction and use of anthocyanins from radish (Raphanus sativus L. var Crimson Gigant) as a natural colorant in yogurt. European Journal of Agriculture and Food Sciences, 4, 26-33. [CrossRef]
- 20. Giusti, M.M., Wrolstad, R.E. (1996). Characterization of red radish anthocyanins. Journal of Food Science, 61, 322-326. [CrossRef]
- 21. Giusti, M.M., Rodríguez-Saona, L.E., Baggett, J.R., Reed, G.L., Durst, R.W., Wrolstad, R.E. (1998). Anthocyanin pigment composition of red radish cultivars as potential food colorants. Journal of Food Science, 63, 219-224. [CrossRef]
- 22. Wentian, C., Eric, K., Jingyang, Y., Shuqin, X., Biao, F., Xiaoming, Z. (2016). Improving red radish anthocyanin yield and off flavor removal by acidified aqueous organic based medium. RSC Advances, 6, 97532-97545. [CrossRef]
- 23. Singh, B.K., Koley, T.K., Karmakar, P., Tripathi, A., Singh, B., Singh, M. (2017) Pigmented radish (Raphanus sativus): Genetic variability, heritability and interrelationships of total phenolics, anthocyanins and antioxidant activity. Indian Council of Agricultural Research, 87, 1600-1606. [CrossRef]
- 24. Jakmatakul, R., Suttisri, R., Tengamnuay, P. (2009). Evaluation of antityrosinase and antioxidant activities of Raphanus sativus root: comparison between freeze-dried juice and methanolic extract. The Thai Journal of Pharmaceutical Sciences, 33, 22-30. [CrossRef]
- 25. Yongpradoem, P., Weerapreeyakul, N. (2020). Evaluation of antioxidant activity and inhibition of tyrosinase activity of Raphanus sativus var. caudatus Alef extract. Walailak Journal of Science and Technology, 17, 838-850. [CrossRef]
- 26. Sungthong, B., Phadungkit, M. (2015). Anti-tyrosinase and DPPH radical scavenging activities of selected Thai herbal extracts traditionally used as skin toner. Pharmacognosy Journal, 7, 97-101.