KAZAKİSTAN’DAN TOPLANAN RHAMNUS CATHARTİCA L. EKSTRAKTLARININ BİYOKİMYASAL ÖZELLİKLERİ, ANTİMIKROBİYAL, ANTİOKSİDAN VE ANTİPROLİFERATİF AKTİVİTELERİNİN ARAŞTIRILMASI

Year 2025, Volume: 49 Issue: 3, 743 - 755, 19.09.2025

Sofiakhan Alimova , Yessimsiitova Zura , Tutku Tunç , Hülya Özpınar

https://doi.org/10.33483/jfpau.1595627

Abstract

Amaç: Bu araştırmada, Kazakistan'ın Almatı kentinden elde edilen Rhamnus cathartica L. bitkisinin korteks, yaprak ve meyvesinin hekzan, etil asetat ve etanol özütlerinin biyokimyasal bileşimi değerlendirilmiş ve antimikrobiyal, antioksidan ve antiproliferatif aktiviteleri analiz edilmiştir.
Gereç ve Yöntem: Rhamnus cathartica L. korteks, yaprak ve meyve özütü kimyasallarını belirlemek için GC-MS yöntemi kullanıldı. Bitki ekstraktlarının patojen mikroorganizmalara karşı MİK değerlerini değerlendirmek için mikrodilüsyon yöntemi kullanılmıştır. Antioksidan aktivite tayini için DPPH, FRAP ve Toplam flavonoid içeriği belirlenmiştir. Ekstraktların MCF-7, A549, C6 ve WI-38 hücreleri üzerine antiproliferatif etkisi XTT testi ile ölçülmüştür.
Sonuç ve Tartışma: GC-MS sonuçlarına göre; Trans-2-Decenal %2.88 ile en yüksek orana sahiptir. Eicosane %1.71 ile ikinci sıradadır. DPPH, FRAP ve flavonoid değerleri meyve etil asetat ekstresinin (F-EtAc) en güçlü antioksidan aktiviteye sahip olduğunu göstermiştir. F-EtAc ekstresi, seçicilik indeksi tablosuna göre en güçlü antikanser aktivitesine sahiptir (WI-38/MCF-7: 12.67, WI-38/C6: 31.38, WI-38/A549: 8.55). Tüm bitki ekstraktları, referans kaynak değerlerine göre Staphylococcus aureus'a karşı anlamlı MİK değerlerine ulaşmıştır. Korteks ve yaprağın etil asetat içindeki MİK değeri 0.078 mg/ml, korteks ve yaprak ekstraktları Bacillus cereus için anlamlı antimikrobiyal aktiviteye (0.312 mg/ml) sahipken, meyveler zayıf etkili MİK değerlerine ulaşmıştır (Anlamlı=MİK <0.1 mg/ml). Bu çalışmanın sonuçlarna göre, Rhamnus cathartica L. bitki ekstraktlarının biyolojik özellikler açısından aktif bir içeriğe sahip olduğu söylenebilir.

Keywords

Antimikrobiyal , antioksidan , antiproliferatif , Rhamnus cathartica

INVESTIGATION OF BIOCHEMICAL PROPERTIES, ANTIMICROBIAL, ANTIOXIDANT AND ANTIPROLIFERATIVE ACTIVITY OF THE RHAMNUS CATHARTICA L. EXTRACTS COLLECTED FROM KAZAKHSTAN

Abstract

Objectives: This research evaluated the biochemical composition of hexane, ethyl acetate, and ethanol extracts and analyzed the antimicrobial, antioxidant and antiproliferative activities of the cortex, leaf, and fruit of the Rhamnus cathartica L. plant from Almaty, Kazakhstan.
Material and Method: The GC-MS method was used to evaluate the chemicals of Rhamnus cathartica L. cortex, leaf, and fruit extracts. The microdilution technique was used to evaluate the MIC values of plant extracts against pathogenic microorganisms. DPPH, FRAP, and Total Flavonoid Content were evaluated for antioxidant activity. The antiproliferative effect of the extracts on MCF-7, A549, C6 and WI-38 cells was measured by XTT assay.
Result and Discussion: According to GC-MS results, Trans-2-Decenal had the highest proportion at 2.88%. Eicosane is second at 1.71%. DPPH, FRAP and flavonoid values showed that the fruit ethyl acetate extract (F-EtAc) had the strongest antioxidant activity. F-EtAc extract had the strongest anticancer activity according to the selectivity index table (WI-38/MCF-7: 12.67, WI-38/C6: 31.38, WI-38/A549: 8.55). All plant extracts had high MIC values against Staphylococcus aureus based on reference source values. The MIC value of cortex and leaf in ethyl acetate was 0.078 mg/ml, cortex and leaf extracts had strong antimicrobial activity (0.312 mg/ml) for Bacillus cereus, while fruits had weakly effective MIC values (Significant=MIC <0.1 mg/ml). According to the results of this study, it can be said that Rhamnus cathartica L. plant extracts have an active content in terms of biological properties.

Keywords

Antimicrobial , antioxidant , antiproliferative , Rhamnus cathartica

Thanks

Çalışmamızın antioksidan deneylerinde bize yardımcı olan Dr. Ceylan HEPOKUR'a teşekkür ederiz.

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