Development and optimization of biotinoyl tripeptide-1 loaded solid lipid nanoparticles for protection against heat induced hair damage

Mahmut Ozan Toksoy; Uğur Şeker; Şeyda Akkuş Arslan

Development and optimization of biotinoyl tripeptide-1 loaded solid lipid nanoparticles for protection against heat induced hair damage

Abstract

Aims: This study aimed to develop and optimize biotinoyl tripeptide-1 (BTP)-loaded solid lipid nanoparticles (SLNs) and to evaluate their protective efficacy against heat-induced structural and thermal damage in hair fibers. Methods: A Box-Behnken experimental design was employed to optimize the formulation. The optimized BTP-SLNs were characterized for particle size, Polydispersity Index (PDI), zeta potential, and entrapment efficiency. Hair samples were exposed to repeated thermal cycles at 200°C over 15 days to simulate the thermal straightening damage. Morphological and thermal damage were also investigated using scanning electron microscopy (SEM) and differential scanning calorimetry (DSC). Results: The optimized BTP-SLN formulation demonstrated a particle size of 207.3 nm, PDI of 0.297, zeta potential of -10.6 mV, and an entrapment efficiency of 46.2%. Heat-treated control samples exhibited pronounced cuticle lifting, cortical disruption, and decreased α-keratin denaturation enthalpy. Empty-SLNs provided partial surface-level protection; the incorporation of BTP significantly enhanced thermal resistance. Additionally, BTP-SLN-treated hair showed improved cuticle integrity, reduced microfibrillary separation, increased denaturation peak intensity, and delayed onset of thermal degradation. Conclusion: BTP-loaded SLNs demonstrated protective effects against heat-induced hair damage, likely through a dual mechanism involving lipid barrier formation and stabilization of the keratin matrix. These findings suggest that BTP-SLNs represent a promising strategy for improving thermal protection in cosmetic hair care applications.

Keywords

Ethical Statement

Ethics Committee Approval: Our study is a laboratory study. No patients and/or living tissues were used. Therefore, ethics committee approval is not required. Informed Consent: This study is a laboratory study. No human and/or human participants were used. Therefore, no written informed consent form was obtained.

Thanks

The authors would like to thank the DÜBTAM Laboratories for providing laboratory infrastructure and technical support throughout this study.

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Details

Primary Language

English

Subjects

Pharmaceutical Sciences, Pharmaceutical Delivery Technologies

Journal Section

Research Article

Authors

Mahmut Ozan Toksoy ^*
0000-0002-1040-9286
Türkiye

Uğur Şeker
0000-0002-1693-6378
Türkiye

Şeyda Akkuş Arslan
0000-0002-0109-7372
Türkiye

Publication Date

May 19, 2026

Submission Date

March 2, 2026

Acceptance Date

May 8, 2026

Published in Issue

Year 2026 Volume: 9 Number: 3

IZ

https://izlik.org/JA93CJ42UJ

Cite

RIS / Bibtex

APA

Toksoy, M. O., Şeker, U., & Akkuş Arslan, Ş. (2026). Development and optimization of biotinoyl tripeptide-1 loaded solid lipid nanoparticles for protection against heat induced hair damage. Journal of Health Sciences and Medicine, 9(3), 761-766. https://izlik.org/JA93CJ42UJ

AMA

1.Toksoy MO, Şeker U, Akkuş Arslan Ş. Development and optimization of biotinoyl tripeptide-1 loaded solid lipid nanoparticles for protection against heat induced hair damage. J Health Sci Med / JHSM. 2026;9(3):761-766. https://izlik.org/JA93CJ42UJ

Chicago

Toksoy, Mahmut Ozan, Uğur Şeker, and Şeyda Akkuş Arslan. 2026. “Development and Optimization of Biotinoyl Tripeptide-1 Loaded Solid Lipid Nanoparticles for Protection Against Heat Induced Hair Damage”. Journal of Health Sciences and Medicine 9 (3): 761-66. https://izlik.org/JA93CJ42UJ.

EndNote

Toksoy MO, Şeker U, Akkuş Arslan Ş (May 1, 2026) Development and optimization of biotinoyl tripeptide-1 loaded solid lipid nanoparticles for protection against heat induced hair damage. Journal of Health Sciences and Medicine 9 3 761–766.

IEEE

[1]M. O. Toksoy, U. Şeker, and Ş. Akkuş Arslan, “Development and optimization of biotinoyl tripeptide-1 loaded solid lipid nanoparticles for protection against heat induced hair damage”, J Health Sci Med / JHSM, vol. 9, no. 3, pp. 761–766, May 2026, [Online]. Available: https://izlik.org/JA93CJ42UJ

ISNAD

Toksoy, Mahmut Ozan - Şeker, Uğur - Akkuş Arslan, Şeyda. “Development and Optimization of Biotinoyl Tripeptide-1 Loaded Solid Lipid Nanoparticles for Protection Against Heat Induced Hair Damage”. Journal of Health Sciences and Medicine 9/3 (May 1, 2026): 761-766. https://izlik.org/JA93CJ42UJ.

JAMA

1.Toksoy MO, Şeker U, Akkuş Arslan Ş. Development and optimization of biotinoyl tripeptide-1 loaded solid lipid nanoparticles for protection against heat induced hair damage. J Health Sci Med / JHSM. 2026;9:761–766.

MLA

Toksoy, Mahmut Ozan, et al. “Development and Optimization of Biotinoyl Tripeptide-1 Loaded Solid Lipid Nanoparticles for Protection Against Heat Induced Hair Damage”. Journal of Health Sciences and Medicine, vol. 9, no. 3, May 2026, pp. 761-6, https://izlik.org/JA93CJ42UJ.

Vancouver

1.Mahmut Ozan Toksoy, Uğur Şeker, Şeyda Akkuş Arslan. Development and optimization of biotinoyl tripeptide-1 loaded solid lipid nanoparticles for protection against heat induced hair damage. J Health Sci Med / JHSM [Internet]. 2026 May 1;9(3):761-6. Available from: https://izlik.org/JA93CJ42UJ