HIV pozitif hastalarda HHV-6 prevalansı ve varyant tiplerinin belirlenmesi

Year 2020, , 12 - 15, 15.01.2020

Gülnur Tarhan , Neziha Yılmaz , Salih Cesur

https://doi.org/10.32322/jhsm.605654

Abstract

Amaç: Bu çalışmanın amacı HIV enfeksiyonu pozitif
saptanan hastalarda HHV-6 DNA sıklığının, HHV-6 DNA’sı pozitif saptanan
olgularda ise HHV-6 varyant türünün (varyant A veya varyant B) belirlenmesiydi.

Gereç
ve Yöntem: Çalışmaya 20 HIV pozitif hasta
ile 57 HIV negatif sağlıklı birey dahil edildi. Hasta ve kontrol grubunda HHV-6
DNA’sı polimeraz zincir reaksiyonu (PZR) ile araştırıldı. HHV-6 DNA pozitif
saptanan olgularda HHV-6 varyant türü restriksiyon parça uzunluğu polimorfizmi (restriction
fragment lenght polymorphisim:  RFLP) ile
araştırıldı. İstatistiksel değerlendirme SPSS programında yapıldı, p≤0.05
istatistiksel olarak anlamlı kabul edildi.

Bulgular: HIV pozitif hastalarda HHV-6 DNA pozitifliği %45
(9/20), HIV negatiflerde ise %33,3 (19/57) olarak belirlendi. Toplamda
pozitiflik oranı %36,3 (28/77) idi. HIV pozitif hastalarda HHV-6 varyant B
saptanırken, varyant A saptanmadı.

Sonuç: HIV pozitif hastalarda HHV-6 seropozitifliği ve
varyant tiplerinin öneminin belirlenmesi için daha fazla sayıda olgu içeren başka
klinik çalışmalara gereksinim olduğu görüşündeyiz.

Keywords

HIV, HHV-6, varyant A, varyant B, koenfeksiyon

The prevalence of HHV-6 and variant type in HIV positive patients

Abstract

Objective: The aim of this
study was to determine the frequency of HHV-6 DNA in patients with HIV positive
infection and HHV-6 variant type (variant A or variant B) in patients with HHV-6
DNA positive

Material and Method: Twenty
HIV-positive patients and 57 HIV negative healthy subjects were included in the
study. HHV-6 DNA was investigated by polymerase chain reaction (PCR) in the
patient and control groups. In cases with HHV-6 DNA positive, HHV-6 variant was
investigated with restriction fragment length polymorphism (RFLP). Statistical
analysis was performed using SPSS program, p≤0.05 was considered statistically
significant.

Results: HHV-6 DNA positivity was found
to be 45% (9/20) in HIV positive patients and 33.3% (19/57) in HIV negative
patients. Overall, the positivity rate was 36.3% (28/77). HHV-6 variant B was
detected in HIV positive patients, but variant A was not.

Conclusion: We believe that
further clinical studies involving more cases are needed to determine the
importance of HHV-6 seropositivity and variant types in HIV positive patients.

Keywords

HIV, HHV-6, varyant A, varyant B, coinfection

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