Optimum Conditions for LDPE Strips Biodegradation by Local Bacterial Isolates

Year 2015, Volume: 10 Issue: 4, 399 - 407, 30.10.2015

Amel Ali Hussain Saad H. Khudhair İthar K. Al-mayaly

Abstract

Optimum
conditions (Temperature, pH and incubation time) were studied for selected
bacterial isolates Pseudomonas fluorescens, Pseudomonas aeruginosa and
Acinetobacter ursingii to biodegrade the low density polyethylene (LDPE) by
using mineral salts medium (MSM) contains one pieces of LDPE strips (1cm ×5cm) using bacterial growth and amount of dissolved carbon
dioxide as an indicator for ability of degradation. The results have been
revealed that the above three bacterial isolates were shown high ability to degrade
the LDPE strips after 15 days of incubation in liquid mineral salts medium pH 7
in 30˚C, the growth was 0.351, 0.272 and 0.241,
respectively, while the amount of dissolved CO₂ was 1.18, 1.05 and 0.95
g/l, respectively. The results of FTIR spectrum of LDPE strips which treated
with three bacterial isolates were shown different changes in transmission
bands for active groups of LDPE strips. Pseudomonas fluorescens isolates
was shown the highest % of C-H group biodegradation (40.47%) compared with the
other two isolates 35.89% and 30.55%, respectively.

Keywords

LDPE biodegradation, optimum condition, CO2, FTIR

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