Relation between IgG AND IgM Antibody Titres against Helicobacter pylori in Serum and Severity of Gastritis

Year 2015, Volume: 10 Issue: 4, 460 - 463, 30.10.2015

Kopaçi Ermela Rexha Tefta Lika Mirela

Abstract

Helicobacter pylori has been established as an important
etiological factor for chronic gastritis and duodenal ulcer. It is also
associated with gastric ulcer and gastric cancer. An easier and cheaper way to
diagnose Helicobacter pylori is to test for antibodies to the infection. Aim:
Using IgG to diagnose Helicobacter pylori infection Method. A blood sample of
all patients selected for endoscopy was taken and serum was stored at normal
temperature for 30 minutes. After this, the blood sample was treated with
peroxydasis and has been conjugated with IgG, antibodies for 30 minutes. After
rinsing, every sample was treated with tetramethyl benzidine for 30 minutes.
The wavelength of measurement absorbance was 450nm.Antibody index of each
sample was calculated by dividing the optical density (OD) value of each sample
by for cut off value, IgG and IgM was: negative result ≤ 1,7: positive result
≥2,3: falls result 1.8-2.2. Conclusion: Sera IgG can be form of diagnostic if
Helicobacter pylori infection (p=0.04). Sera positive IgG is related with
gastritis (P<0.001). Sera positive IgG is not related with the age of sample
of this study

Keywords

Helicobacter pylori infection, IgG, Elisa, mucosal gastritis

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