Characterization of Phosphate Solubilising Bacteria from Limestone Quarry in Cirebon Indonesia

Year 2016, Volume: 11 Issue: 4, 312 - 317, 30.12.2016

Emil Wahdi Nisa Rachmania Mubarik Rahayu Widyastuti

Abstract

Limestone quarry has
low available P for plant on land revegetation process. Therefore, phosphate-solubilising
bacteria could be a solution to dissolve phosphate in limestone in order to add
phosphate availability. The research was aimed to characterize and identify the
phosphate-solubilising bacteria adapted in limestone quarry. Isolation was performed
using Pikovskaya medium which obtained four isolates bacteria had halo-zone around
the colony.  The ability to dissolve
phosphate was examined using five soil samples from different region of
limestone quarry. The result showed index of P solubility between 1.63 to 3.42.
Identification bacterial isolates by using 16S rRNA gene showed that the
isolates have similarity with Pseudomonas
mosselii QC5A.1, Pseudomonas mosselii
QC5B.1, Pantoea ananatis QC5C.1, and Pseudomonas oryzihabitans QC5B.3. 

Keywords

Limestone quarry, phosphate-solubilising bacteria, Pseudomonas, Pantoea, 16S rRNA

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