Antibacterial, Antioxidant and DNA Interaction Properties of Cistus creticus L. Extracts

Year 2019, Volume: 14 Issue: 3, 110 - 115, 30.09.2019

Dudu Duygu Kılıç Belgin Sırıken Ömer Ertürk Güler Tanrıkulu Melek Gül Ceren Baskan

Abstract

Medicinal plants
are a natural source that possesses significant biological functions. Cistus species which are medical plant
are of antibacterial, antioxidant and cytotoxic activities. This study was perfpormed
to investigate the antibacterial, antioxidant and DNA interaction properties of
Cistus creticus extracts.

Samples of Cistus creticus L. were obtained in May
2018 in the region of Amasya. The different extract of Cistus criticus leaves was obtained by using soxhlet apparatus for
4 hours. Antibacterial activity of extracts was tested against four Gram positive
and four Gram negative by the disc diffusion method. Antioxidant activities of
extracts were determined with DPPH radical scavenging, ferric reducing and
metal chelating methods. The ability to repair the plasmid DNA breaks created
by hydroxyl radicals was also determine using pUC18 plasmid DNA. 

As a result,
Cistus creticus. extracts has strong
inhibitory activity against all tested bacteria. The chloroform extract was
also very effective against Gram positive bacteria especially Bacillus cereus. Among the Gram negative
bacteria, the most susceptible bacterium was identified as Pseudomonas aeruginosa. Moreover, ethanol extracts had repair
effects on plasmid DNA in H₂O₂ condition. Among the
different extracts of Cistus
creticus chloroform extract showed the highest in vitro
antioxidant
activity.

In this study, Cistus creticus collected from Amasya has shown significant
antibacterial, antioxidant and DNA interaction activity and could be therefore
a useful source in the discovery of new antibacterial and antioxidant
compounds. However, the use of extracts as a complement to the treatment
requires further research to thoroughly understand the activities and
interactions with recommended medicines.

Keywords

Cistus creticus L. Antioxidant, Antimicrobial, DNA interaction

References

• Prof. Dr. Birsen Aydı[e-mail]
• Assist Prof Melek Çö[e-mail]