Oksaliplatin ve Naringin’in Dalak Dokusu Üzerine Etkilerinin Rat Modelinde Değerlendirilmesi

Abstract

Oksaliplatin (OPL), kolorektal kanser tedavisinde yaygın olarak kullanılan platin bazlı kemoterapötik ajandır. Naringin (NRG) ise çeşitli biyolojik ve farmakolojik özelliklere sahip olan antioksidan bir bileşiktir. Sunulan çalışmada OPL ve NRG uygulamalarının ratların dalak dokusu üzerine etkileri değerlendirilmiştir. Ratlara çalışmanın 1. ve 2. günleri ile 5. ve 6. günlerinde oral olarak 50 veya 100 mg/kg vücut ağırlığı NRG verildikten 30 dakika sonra 4 mg/kg vücut ağırlığı OPL periton içine enjekte edildi. Daha sonra dalak dokusunda malondialdehit (MDA) ve glutatyon (GSH) seviyeleri ile nükleer faktöreritroid faktör 2-ilişkili faktör 2 (Nrf2), hem oksijenaz-1 (HO-1), nükleer faktör kappa B (NF-B), tümör nekroz faktörü alfa (TNF-), B hücreli lenfoma 2-ilişkili X proteini (Bax) ve Kaspaz-3 mRNA transkript seviyeleri analiz edilmiştir. Elde edilen veriler OPL tedavisi sonrası artan MDA seviyelerini NRG’nin azalttığını göstermiştir. Ayrıca OPL uygulaması ile azalan GSH, Nrf2 ve HO-1 seviyelerinin NRG tedavisi sonrası artış gösterdiği belirlendi. Ayrıca OPL ile tetiklenen NF-B, TNF-, Bax ve Kaspaz-3 ekspresyonlarının NRG tarafından aşağı yönlü düzenlendiği tespit edilmiştir. Sonuç olarak, OPL’nin dalak dokusunda oksidatif stres, enflamasyon ve apoptozu tetiklediği buna karşın NRG’nin OPL ile tetiklenen bu olayları tersine çevirdiği sonucuna varılmıştır.

Keywords

• Apoptoz
• dalak
• naringin
• oksaliplatin
• oksidatif stres

Evaluation of The Effects of Oxaliplatin and Naringin on Spleen Tissue in a Rat Model

Abstract

Oxaliplatin (OPL) is a platinum-based chemotherapeutic agent widely used in the treatment of colorectal cancer. Naringin (NRG) is an antioxidant compound with various biological and pharmacological properties. In the present study, the effects of OPL and NRG administrations on spleen tissue of rats were evaluated. On the 1st and 2nd days of the study and on the 5th and 6th days of the study, 30 minutes after oral administration of 50 or 100 mg/kg body weight NRG, 4 mg/kg body weight OPL was injected intraperitoneally. Then, malondialdehyde (MDA) and glutathione (GSH) levels and nuclear factorerythroid factor 2-related factor-2 (Nrf2), heme oxygenase-1 (HO-1), nuclear factor kappa-B (NF-B), tumor necrosis factoralpha (TNF-), B-cell lymphoma 2-associated X protein (Bax), and Caspase-3 mRNA transcript levels were analyzed in spleen tissue. The data obtained showed that NRG reduced the increased MDA levels after OPL treatment. It was also determined that GSH, Nrf2 and HO-1 levels, which decreased with OPL administration, increased after NRG treatment. Moreover, OPLinduced NF-B, TNF-, Bax and Caspase-3 expressions were downregulated by NRG. Taken together, it was concluded that OPL triggered oxidative stress, inflammation and apoptosis in spleen tissue whereas NRG reversed these OPL-induced events.

Keywords

• Apoptosis
• naringin
• oxaliplatin
• oxidative stress
• spleen

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