Siyatik Sinir Ligasyonunda Bacopa monnieri L.’nin Tedavi Potansiyeli: Rejenerasyon ve Oksidatif Stresin Modülasyonu

Abstract

Bacopa monnieri L. nöronal rejenerasyon ve sinaptik aktivite üzerinde olumlu etkileri olduğu bilinen nöroprotektif özelliğe sahip bir bitkidir. Bu çalışmada Bacopa monnieri L. ekstresinin periferik sinir hasarı modeli olan siyatik sinir hasarında rejenerasyon süreci üzerine etkileri araştırılmıştır. Çalışma kapsamında Sprague–Dawley ırkı 18 adet sıçan her birinde 6 hayvan bulunan 3 gruba ayrıldı. Pozitif kontrol ve tedavi gruplarına siyatik sinir hasarı oluşturmak amacıyla deneyin başlangıcında cerrahi olarak siyatik sinir ligasyonu uygulandı. Tedavi grubuna cerrahi uygulama sonrası 14. günden itibaren 15 gün boyunca 400 mg/kg/gün ekstrakt uygulandı. Sıçanlardan alınan serum örneklerinde oksidatif stres durumu biyokimyasal olarak araştırıldı. İmmunohistokimyasal olarak ise siyatik sinir çevresindeki kaslarda TNF-α ve iNOS ölçümü gerçekleştirildi. Elde edilen sonuçlar incelendiğinde kontrol gruplarına göre tedavi grubunda antioksidan kapasite önemli ölçüde artmıştır. Oksidan durumun ise tedavi grubunda PC grubundan daha düşük olduğu görülmüştür. Immunohistochemical incelemelerde PC grubunda TNF-α ve iNOS immunoreaktivitesini artarken tedavi grubunda bu inflamasyon belirteçlerinin immunoreaktivitesinin düştüğü belirlenmiştir. Sonuç olarak Bacopa monnieri L. ekstresi periferik sinir hasarında antioksidan ve antiinflamatuar süreçleri düzenleyerek iyileşmeye katkı sağlayabilir.

Keywords

• Antiinflamatuar
• antioksidan
• Bacopa monnieri L.
• periferik sinir hasarı
• siyatik sinir bağlama

Therapeutic Potential of Bacopa monnieri L. in Sciatic Nerve Ligation: Modulation of Regeneration and Oxidative Stress

Abstract

Bacopa monnieri L. is a plant known for its neuroprotective properties with positive effects on neuronal regeneration and synaptic activity. In this study, the effects of Bacopa monnieri L. extract on the regeneration process in sciatic nerve injury, which is a peripheral nerve injury model, were investigated. Within the scope of this study, 18 Sprague–Dawley rats, each group containing six animals, were divided into three groups. Surgical ligation of the sciatic nerve was performed at the beginning of the experiment to induce sciatic nerve injury in the positive control and treatment groups. Following surgical intervention, the treatment group received a daily dose of 400 mg/kg extract for 15 days starting from the 14th day post surgery. The oxidative stress status in serum samples obtained from rats was investigated biochemically. Immunohistochemical measurements of tumor necrosis factor alpha and inducible nitric oxide synthase were also performed in the muscles surrounding the sciatic nerve. Upon examination of the results, it was observed that the treatment group exhibited a significant increase in antioxidant capacity compared to the control groups. Furthermore, the oxidant status in the treatment group was lower than that in the positive control group. Immunohistochemical examinations revealed an increase in tumor necrosis factor alpha- and inducible nitric oxide synthase immunoreactivity in the positive control group, while a decrease in the immunoreactivity of these inflammation markers was observed in the treatment group. In conclusion, Bacopa monnieri L. extract may contribute to recovery in peripheral nerve injury by regulating antioxidant and anti-inflammatory processes.

Keywords

• İmmunohistokimyasal
• antioxidant
• Bacopa monnieri L.
• peripheral nerve injury
• sciatic nerve ligation

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