Over İskemi-Reperfüzyon Hasarında Sefarantinin Terapötik Potansiyeli: Rat Over Torsiyon-Detorsiyon Modelinden Bulgular

Abstract

Bu çalışmada, Cepharanthine’in (CEP) over torsiyonu-detorsiyonu (T/D) sıçan modelinde iskemi-reperfüzyon (İ/R) hasarını hafifletici potansiyel koruyucu etkileri değerlendirilmiştir. Yirmi dört dişi Sprague-Dawley sıçanı rastgele üç deney grubuna ayrılmıştır: sham, T/D ve CEP 10 mg/kg. Çalışmada, over doku örneklerinde malondialdehit (MDA), miyeloperoksidaz (MPO) ve süperoksit dismutaz (SOD) gibi oksidatif stres biyobelirteçleri ile tümör nekroz faktör-alfa (TNF-α) ve interlökin-1 beta (IL-1β) gibi proinflamatuar medyatörler incelenmiştir. T/D grubunda anlamlı düzeyde oksidatif stres gözlenmiş; bu, artmış MDA ve MPO düzeyleri ve azalmış SOD aktivitesi ile karakterize edilmiştir. Aynı zamanda TNF-α ve IL-1β düzeylerinin artmasıyla belirgin inflamatuar yanıtlar gözlenmiştir (p<0.05, Sham ile karşılaştırıldığında). CEP uygulaması, bu zararlı etkileri azaltmış; oksidatif stres ve inflamatuar sitokin düzeylerini anlamlı şekilde düşürmüş ve SOD aktivitesini eski haline getirmiştir (p<0.05, T/D ile karşılaştırıldığında). CEP, over İ/R hasarında oksidatif stres ve inflamasyonu etkili bir şekilde azaltarak, over torsiyonu ve bununla ilgili jinekolojik aciller için terapötik potansiyelini ortaya koymaktadır. Klinik uygulamalarının araştırılması için daha ileri çalışmalara ihtiyaç vardır.

Keywords

• Cepharanthine
• İskemi reperfüzyon
• Over torsiyon detorsiyon
• Oksidatif stres
• İnflamasyon

Therapeutic Potential of Cepharanthine in Ovarian Ischemia-Reperfusion Injury: Insights from a Rat Ovarian Torsion-Detorsion Model

Abstract

This study evaluated the potential protective effects of Cepharanthine (CEP) in mitigating ischemia-reperfusion (I/R) injury of an ovarian torsion/detorsion (T/D) rat model. Twenty-four female Sprague-Dawley rats were randomized into three experimental groups: sham, T/D, and CEP 10 mg/kg. The study examined ovarian tissue samples to measure oxidative stress biomarkers, such as malondialdehyde (MDA), myeloperoxidase (MPO), and superoxide dismutase (SOD), as well as proinflammatory mediators, specifically tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β). The T/D group exhibited significant oxidative stress, characterized by elevated MDA and MPO levels and low SOD activity, along with heightened inflammatory responses, as indicated by elevated TNF-α and IL-1β levels (p<0.05 vs. Sham). CEP administration mitigated these deleterious effects, significantly reducing oxidative stress and inflammatory cytokine levels while restoring SOD activity (p<0.05 vs. T/D). CEP effectively attenuates oxidative stress and inflammation in ovarian I/R injury, highlighting its therapeutic potential for ovarian torsion and related gynecological emergencies. Further studies are warranted to explore its clinical applicability.

Keywords

• Cepharanthine
• Ischemia reperfusion
• Ovarian Torsion detorsion
• Oxidative stress

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