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Koenzim Q10 ve Hesperidinin Ratlarda Alt Ekstremite İskemi-Reperfüzyon Hasarında TAS ve TOS Düzeylerine Etkisi

Year 2022, Volume: 2 Issue: 2, 90 - 95, 28.09.2022

Betül Apaydın Yıldırım

Abstract

Alt ekstremite iskemi reperfüzyon (İR) hasarında Koenzim Q10 (CoQ10) ve Hesperidinin (HESP) TAS ve TOS düzeylerine etkilerinin belirlenmesi amaçlandı. Çalışmada 28 adet 200-250 gr ağırlığında Sprague- Dawley erkek ratlar; Kontrol (K), İskemi reperfüzyon (İR), İR+CoQ10 ve İR+HESP grubu olmak üzere 4 gruba ayrıldı. İR+CoQ10 grubuna iskemi oluşturulmadan önce 10mg/kg dozunda 8 saat arayla 3 kez 0.5mL CoQ10, İR+HESP grubuna ise100mg/kg dozunda 8 saat arayla 3 kez 0.5mL Hesperidin gastrik gavaj ile verildi. Kontrol grubu hariç diğer 3 gruba anestezi altında sol alt ekstremitelerine turnike uygulanıp 2 saat iskemi, 2 saat reperfüzyon uygulandı. Çalışma sonunda ratlardan anestezi altında kan örnekleri alınarak total antioksidan seviye (TAS) ve total oksidan statü (TOS) düzeyleri ölçüldü. İR grubunda TOS düzeyi tüm gruplardan yüksek iken, iskemi ile birlikte CoQ10 ve HESP uygulamasının bu düzeyleri İR grubuna göre anlamlı olarak düşürdüğü, TAS düzeylerini ise iskemi ile birlikte uygulanan antioksidanların İR grubuna göre istatistiksel olarak anlamlı olarak yükselttiği tespit edildi. Sonuç olarak ratlarda deneysel olarak oluşturulan alt ekstremite iskemi reperfüzyon hasarında CoQ10 ve HESP TOS düzeyini azaltarak, TAS düzeyini arttırarak oksidatif hasara karşı koruyucu etkiler gösterdiği belirlendi.  

Keywords

Hesperidin iskemi koenzim Q10 reperfüzyon turnike.

References

  • 1. Abbas, A., Iqbal, Z., Abbas, R. Z., Khan, M. K., Khan, J. A., Mahmood, M. S., et al. (2017). In vivo anticoccidial effects of Beta vulgaris (sugar beet) in broiler chickens. Microbial pathogenesis, 111, 139-144.
  • 2. Abd El Megid, A. D., Khaled, M., Emam, M. A., & Adel, A. (2018). Biochemical role of zinc oxide and propolis nanoparticles in protection rabbits against coccidiosis. Benha Veterinary Medical Journal, 34(1), 314-328.
  • 3. Aboelhadid, S. M., El-Ashram, S., Hassan, K. M., Arafa, W. M., & Darwish, A. B. (2019). Hepato-protective effect of curcumin and silymarin against Eimeria stiedae in experimentally infected rabbits. Livestock Science, 221, 33-38.
  • 4. Aboelhadid, S. M., Hashem, S., Abdel-Kafy, E. S., Mahrous, L. N., Farghly, E. M., Abdel-Baki, A. A. S., et al. (2021). Prebiotic supplementation effect on Escherichia coli and Salmonella species associated with experimentally induced intestinal coccidiosis in rabbits. PeerJ, 9, e10714.
  • 5. Abu El Ezz, N., Aboelsoued, D., Hassan, S., Abdel Megeed, K., & El-Metenawy, T. (2020). Therapeutic effect of Moringa oleifera and Thymus vulgaris oils against hepatic coccidiosis in experimentally infected rabbits. Tropical Biomedicine, 37(4), 1018-1028.
  • 6. Ayan, A., Ahmed, I., Khan, J. M., Munir, S., Hussain, M., Khan, A. K., et al. (2020). Hematological changes and comparative efficacy of allopathic and herbal drugs on coccidiosis in rabbits. Baltica, 33(2), 78-99.
  • 7. Benato L, Hastie P, O'Shaughnessy P, Murray JA, Meredith A. Effects of probiotic Enterococcus faecium and Saccharomyces cerevisiae on the faecal microflora of pet rabbits. J Small Anim Pract. 2014 Sep;55(9):442-6.
  • 8. Çam, Y., Atasever, A., Eraslan, G., Kibar, M., Atalay, Ö., Beyaz, L., et al. (2008). Eimeria stiedae: experimental infection in rabbits and the effect of treatment with toltrazuril and ivermectin. Experimental parasitology, 119(1), 164-172.
  • 9. Dorman, H. D., & Deans, S. G. (2000). Antimicrobial agents from plants: antibacterial activity of plant volatile oils. Journal of applied microbiology, 88(2), 308-316.
  • 10. El-Ashram, S. A., Aboelhadid, S. M., Abdel-Kafy, E.-S. M., Hashem, S. A., Mahrous, L. N., Farghly, E. M., et al. (2019). Prophylactic and therapeutic efficacy of prebiotic supplementation against intestinal coccidiosis in rabbits. Animals, 9(11), 965.
  • 11. El-Ghoneimy, A., & El-Shahawy, I. (2017). Evaluation of amprolium and toltrazuril efficacy in controlling natural intestinal rabbit coccidiosis. Iranian journal of veterinary research, 18(3), 164.
  • 12. Gibson GR, Hutkins R, Sanders ME, Prescott SL, Reimer RA, Salminen SJ, Scott K, Stanton C, Swanson KS, Cani PD, Verbeke K, Reid G. Expert consensus document: The International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of prebiotics. Nature Reviews Gastroenterol Hepatol. 2017 Aug;14(8):491-502.
  • 13. Hill C, Guarner F, Reid G, Gibson GR, Merenstein DJ, Pot B, Morelli L, Canani RB, Flint HJ, Salminen S, Calder PC, Sanders ME. Expert consensus document. The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nature Reviews Gastroenterol Hepatol. 2014 Aug;11(8):506-14.
  • 14. Indrasanti, D., Indradji, M., Hastuti, S., Aprilliyani, E., Fatikha, F., & Rosyadi, K. (2017). The administration of garlic extract on Eimeria stiedai oocysts and the hematological profile of the coccidia infected rabbits. Media Peternakan, 40(3), 158-164.
  • 15. Jahangiri, F., Razavi, S., & Nazifi, S. (2018). Comparative effect of Melissa officinalis aqueous extract, sulfadimidine, and vitamin E–selenium on antioxidant parameters in rabbit experimental coccidiosis. Comparative Clinical Pathology, 27(2), 371-378.
  • 16. KN, A. M., NM, A. E., & Abdel-Rahman, E. H. (2005). Protective effect of Eimeria stiedae coproantigen against hepatic coccidiosis in rabbits. Journal of the Egyptian Society of Parasitology, 35(2), 581-595.
  • 17. Leone, A., Spada, A., Battezzati, A., Schiraldi, A., Aristil, J., & Bertoli, S. (2015). Cultivation, genetic, ethnopharmacology, phytochemistry and pharmacology of Moringa oleifera leaves: An overview. International journal of molecular sciences, 16(6), 12791-12835.
  • 18. Ogolla, K. O., Gathumbi, P. K., Waruiru, R. M., Okumu, P. O., Chebet, J., & Kitala, P. M. (2018). Efficacy of sulphachloropyrazine, amprolium hydrochloride, trimethoprim-sulphamethoxazole, and diclazuril against experimental and natural rabbit coccidiosis. Journal of veterinary medicine, 2018.
  • 19. Pérez-Fonseca, A., Alcala-Canto, Y., Salem, A. Z., & Alberti-Navarro, A. B. (2016). Anticoccidial efficacy of naringenin and a grapefruit peel extract in growing lambs naturally-infected with Eimeria spp. Veterinary parasitology, 232, 58-65.
  • 20. Petrova, Y. (2019). Pasteurellosıs And Eımerıosıs–Worldwıde Problems In The Rabbıt Farms: A Revıew. Trakia Journal of Sciences, 17(1), 67.
  • 21. Pourali, M., Kermanshahi, H., Golian, A., Razmi, G. R., & Soukhtanloo, M. (2014). Antioxidant and anticoccidial effects of garlic powder and sulfur amino acids on Eimeria-infected and uninfected broiler chickens. Iranian Journal of Veterinary Research, 15(3), 227-232.
  • 22. Qamar, F., Sharif, R., Qamar, M., & Basharat, A. (2013). Comparative efficacy of sulphadimidine sodium, toltrazuril and amprolium for coccidiosis in rabbits. Science International (Lahore), 25(2), 295-298.
  • 23. Rasooli, I., Rezaei, M. B., & Allameh, A. (2006). Ultrastructural studies on antimicrobial efficacy of thyme essential oils on Listeria monocytogenes. International journal of infectious diseases, 10(3), 236-241.
  • 24. Rivero-Perez, N., Hernández-Alvarado, J. L., Valladares-Carranza, B., Delgadillo-Ruiz, L., Ojeda-Ramírez, D., Sosa-Gutiérrez, C. G., et al. (2019). Salix babylonica L. as a natural anticoccidial alternative in growing rabbits. Evidence-Based Complementary and Alternative Medicine, 2019.
  • 25. Sivajothi, S., Reddy, B. S., & Rayulu, V. (2014). Intestinal coccidiosis infection in domestic rabbits. International Journal of Biological Research, 2(2), 48-50.
  • 26. Sivajothi, S., Reddy, B. S., & Rayulu, V. (2016). Study on impression smears of hepatic coccidiosis in rabbits. Journal of Parasitic Diseases, 40(3), 906-909.
  • 27. Sorour, S. S., Abou Asa, S., Elhawary, N. M., Ghazy, E. W., Abd El Latif, A., El-Abasy, M. A., et al. (2018). Anticoccidial and hepatoprotective effects of artemisinin liquid extract, cinnamon essential oil and clove essential oil against Eimeria stiedae infection in rabbits. Tropical biomedicine, 35(4), 926-943.
  • 28. Worku, M., Franco, R., & Baldwin, K. (2009). Efficacy of garlic as an anthelmintic in adult Boer goats. Archives of Biological Sciences, 61(1), 135-140

Year 2022, Volume: 2 Issue: 2, 90 - 95, 28.09.2022

Betül Apaydın Yıldırım

Abstract

It was aimed to determine the effects of Coenzyme Q10 (CoQ10) and Hesperidin (HESP) on TAS and TOS levels in lower extremity ischemia-reperfusion (IR) injury. In the study, 28 Sprague-Dawley male rats weighing 200-250 g were divided into 4 groups as Control (C), Ischemia reperfusion (IR), IR+CoQ10 and IR+HESP groups. Before ischemia was induced in the IR+CoQ10 group, 0.5mL CoQ10 was administered at a dose of 10mg/kg 3 times with 8-hour intervals, and to the IR+HESP group at a dose of 100mg/kg, 0.5mL Hesperidin was administered 3 times at 8-hour intervals by gastric gavage. Except for the control group, a tourniquet was applied to the left lower extremities and 2 hours of ischemia and 2 hours of reperfusion were applied to the other 3 groups under anesthesia. At the end of the study, blood samples were taken from the rats under anesthesia and total oxidant status (TOS) and total antioxidant status (TAS) levels were measured. While TOS level was higher in the IR group than in all groups, it was determined that CoQ10 and HESP application with ischemia significantly decreased these levels compared to the IR group, and antioxidants applied together with ischemia increased the TAS levels statistically significantly compared to the IR group. It was determined that it showed protective effects by decreasing the CoQ10 and HESP TOS level and increasing the TAS level in experimentally induced ischemia-reperfusion injury of the lower extremity in rats.

References

  • 1. Abbas, A., Iqbal, Z., Abbas, R. Z., Khan, M. K., Khan, J. A., Mahmood, M. S., et al. (2017). In vivo anticoccidial effects of Beta vulgaris (sugar beet) in broiler chickens. Microbial pathogenesis, 111, 139-144.
  • 2. Abd El Megid, A. D., Khaled, M., Emam, M. A., & Adel, A. (2018). Biochemical role of zinc oxide and propolis nanoparticles in protection rabbits against coccidiosis. Benha Veterinary Medical Journal, 34(1), 314-328.
  • 3. Aboelhadid, S. M., El-Ashram, S., Hassan, K. M., Arafa, W. M., & Darwish, A. B. (2019). Hepato-protective effect of curcumin and silymarin against Eimeria stiedae in experimentally infected rabbits. Livestock Science, 221, 33-38.
  • 4. Aboelhadid, S. M., Hashem, S., Abdel-Kafy, E. S., Mahrous, L. N., Farghly, E. M., Abdel-Baki, A. A. S., et al. (2021). Prebiotic supplementation effect on Escherichia coli and Salmonella species associated with experimentally induced intestinal coccidiosis in rabbits. PeerJ, 9, e10714.
  • 5. Abu El Ezz, N., Aboelsoued, D., Hassan, S., Abdel Megeed, K., & El-Metenawy, T. (2020). Therapeutic effect of Moringa oleifera and Thymus vulgaris oils against hepatic coccidiosis in experimentally infected rabbits. Tropical Biomedicine, 37(4), 1018-1028.
  • 6. Ayan, A., Ahmed, I., Khan, J. M., Munir, S., Hussain, M., Khan, A. K., et al. (2020). Hematological changes and comparative efficacy of allopathic and herbal drugs on coccidiosis in rabbits. Baltica, 33(2), 78-99.
  • 7. Benato L, Hastie P, O'Shaughnessy P, Murray JA, Meredith A. Effects of probiotic Enterococcus faecium and Saccharomyces cerevisiae on the faecal microflora of pet rabbits. J Small Anim Pract. 2014 Sep;55(9):442-6.
  • 8. Çam, Y., Atasever, A., Eraslan, G., Kibar, M., Atalay, Ö., Beyaz, L., et al. (2008). Eimeria stiedae: experimental infection in rabbits and the effect of treatment with toltrazuril and ivermectin. Experimental parasitology, 119(1), 164-172.
  • 9. Dorman, H. D., & Deans, S. G. (2000). Antimicrobial agents from plants: antibacterial activity of plant volatile oils. Journal of applied microbiology, 88(2), 308-316.
  • 10. El-Ashram, S. A., Aboelhadid, S. M., Abdel-Kafy, E.-S. M., Hashem, S. A., Mahrous, L. N., Farghly, E. M., et al. (2019). Prophylactic and therapeutic efficacy of prebiotic supplementation against intestinal coccidiosis in rabbits. Animals, 9(11), 965.
  • 11. El-Ghoneimy, A., & El-Shahawy, I. (2017). Evaluation of amprolium and toltrazuril efficacy in controlling natural intestinal rabbit coccidiosis. Iranian journal of veterinary research, 18(3), 164.
  • 12. Gibson GR, Hutkins R, Sanders ME, Prescott SL, Reimer RA, Salminen SJ, Scott K, Stanton C, Swanson KS, Cani PD, Verbeke K, Reid G. Expert consensus document: The International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of prebiotics. Nature Reviews Gastroenterol Hepatol. 2017 Aug;14(8):491-502.
  • 13. Hill C, Guarner F, Reid G, Gibson GR, Merenstein DJ, Pot B, Morelli L, Canani RB, Flint HJ, Salminen S, Calder PC, Sanders ME. Expert consensus document. The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nature Reviews Gastroenterol Hepatol. 2014 Aug;11(8):506-14.
  • 14. Indrasanti, D., Indradji, M., Hastuti, S., Aprilliyani, E., Fatikha, F., & Rosyadi, K. (2017). The administration of garlic extract on Eimeria stiedai oocysts and the hematological profile of the coccidia infected rabbits. Media Peternakan, 40(3), 158-164.
  • 15. Jahangiri, F., Razavi, S., & Nazifi, S. (2018). Comparative effect of Melissa officinalis aqueous extract, sulfadimidine, and vitamin E–selenium on antioxidant parameters in rabbit experimental coccidiosis. Comparative Clinical Pathology, 27(2), 371-378.
  • 16. KN, A. M., NM, A. E., & Abdel-Rahman, E. H. (2005). Protective effect of Eimeria stiedae coproantigen against hepatic coccidiosis in rabbits. Journal of the Egyptian Society of Parasitology, 35(2), 581-595.
  • 17. Leone, A., Spada, A., Battezzati, A., Schiraldi, A., Aristil, J., & Bertoli, S. (2015). Cultivation, genetic, ethnopharmacology, phytochemistry and pharmacology of Moringa oleifera leaves: An overview. International journal of molecular sciences, 16(6), 12791-12835.
  • 18. Ogolla, K. O., Gathumbi, P. K., Waruiru, R. M., Okumu, P. O., Chebet, J., & Kitala, P. M. (2018). Efficacy of sulphachloropyrazine, amprolium hydrochloride, trimethoprim-sulphamethoxazole, and diclazuril against experimental and natural rabbit coccidiosis. Journal of veterinary medicine, 2018.
  • 19. Pérez-Fonseca, A., Alcala-Canto, Y., Salem, A. Z., & Alberti-Navarro, A. B. (2016). Anticoccidial efficacy of naringenin and a grapefruit peel extract in growing lambs naturally-infected with Eimeria spp. Veterinary parasitology, 232, 58-65.
  • 20. Petrova, Y. (2019). Pasteurellosıs And Eımerıosıs–Worldwıde Problems In The Rabbıt Farms: A Revıew. Trakia Journal of Sciences, 17(1), 67.
  • 21. Pourali, M., Kermanshahi, H., Golian, A., Razmi, G. R., & Soukhtanloo, M. (2014). Antioxidant and anticoccidial effects of garlic powder and sulfur amino acids on Eimeria-infected and uninfected broiler chickens. Iranian Journal of Veterinary Research, 15(3), 227-232.
  • 22. Qamar, F., Sharif, R., Qamar, M., & Basharat, A. (2013). Comparative efficacy of sulphadimidine sodium, toltrazuril and amprolium for coccidiosis in rabbits. Science International (Lahore), 25(2), 295-298.
  • 23. Rasooli, I., Rezaei, M. B., & Allameh, A. (2006). Ultrastructural studies on antimicrobial efficacy of thyme essential oils on Listeria monocytogenes. International journal of infectious diseases, 10(3), 236-241.
  • 24. Rivero-Perez, N., Hernández-Alvarado, J. L., Valladares-Carranza, B., Delgadillo-Ruiz, L., Ojeda-Ramírez, D., Sosa-Gutiérrez, C. G., et al. (2019). Salix babylonica L. as a natural anticoccidial alternative in growing rabbits. Evidence-Based Complementary and Alternative Medicine, 2019.
  • 25. Sivajothi, S., Reddy, B. S., & Rayulu, V. (2014). Intestinal coccidiosis infection in domestic rabbits. International Journal of Biological Research, 2(2), 48-50.
  • 26. Sivajothi, S., Reddy, B. S., & Rayulu, V. (2016). Study on impression smears of hepatic coccidiosis in rabbits. Journal of Parasitic Diseases, 40(3), 906-909.
  • 27. Sorour, S. S., Abou Asa, S., Elhawary, N. M., Ghazy, E. W., Abd El Latif, A., El-Abasy, M. A., et al. (2018). Anticoccidial and hepatoprotective effects of artemisinin liquid extract, cinnamon essential oil and clove essential oil against Eimeria stiedae infection in rabbits. Tropical biomedicine, 35(4), 926-943.
  • 28. Worku, M., Franco, R., & Baldwin, K. (2009). Efficacy of garlic as an anthelmintic in adult Boer goats. Archives of Biological Sciences, 61(1), 135-140
There are 28 citations in total.

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Primary Language Turkish
Subjects Veterinary Sciences
Journal Section Research Articles
Authors

Betül Apaydın Yıldırım

Publication Date September 28, 2022
Submission Date August 10, 2022
Published in Issue Year 2022 Volume: 2 Issue: 2

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EndNote Apaydın Yıldırım B (September 1, 2022) Koenzim Q10 ve Hesperidinin Ratlarda Alt Ekstremite İskemi-Reperfüzyon Hasarında TAS ve TOS Düzeylerine Etkisi. Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi 2 2 90–95.
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