Molecular Diagnosis Of Clostridium piliforme, The Causative Agent Of Tyzzer’s Disease In Mice And Rats

Year 2024, Volume: 4 Issue: 2, 78 - 82, 19.09.2024

Ufuk Ülker , Sibel Kızıl , Efsun Melike Çeçen , Elif Aydın

https://doi.org/10.62425/jlasp.1426993

Abstract

Tyzzer’s disease, caused by Clostridium piliforme (C. piliforme), occurs in many species of mammals mainly in rodents, rabbits and foals is an acute, epizootic bacterial disease that can result in mortality. In our study, C. piliforme was investigated in feces samples from mice and rats in a laboratory experimental animals center by PCR technique. C. piliforme was detected in 44 (83.01%) of a total of 53 mouse feces of different ages. Of the mice found positive, 28 (73.6%) were female and 16 (88.8%) were male. C. piliforme was detected in 40 (90.9%) of a total of 44 rats feces of different ages. Of the rats found positive, 25 (96.1%) were female and 15 (83.3%) were male. C. piliforme, the causative agent of Tyzzer's disease, was detected at a high rate in mice and rats examined by the classical PCR method. When evaluated in terms of gender, 28 (73.6%) of the 40 mice positive for Tyzerr's disease were female and 16 (88.8%) were male; While 25 (96.1%) of the rats were female, 15 (83.3%) were male. As a result, Tyzerr's disease caused by C. piliforme was detected at a high rate in mice and rats by classical PCR. When evaluated by age, the disease was detected to be higher in mice at the ages of 4, 5 and 8, and in rats at the ages of 4, 5, 6 and 7. Due to the reliability of the analyses and the high risk of transmission to other animals in centers where laboratory animals are raised, Tyzzer's disease should be checked and necessary precautions should be taken to prevent the disease.

Keywords

Clostridium piliforme, PCR, Tyzzer’s Disease, rat

Ethical Statement

This study was approved by the Veterinary Control Central Research Institute Directorate, Local Ethical Committee Decision date and no. 08.11.2022/43.

Fare ve Ratlarda Tyzerr’s Hastalığı Etkeni Clostridium piliforme’nin Moleküler Teşhisi

Abstract

Clostridium piliforme (C. piliforme)'nin neden olduğu Tyzzer hastalığı, başta kemirgenler, tavşanlar ve taylar olmak üzere birçok memeli türünde ortaya çıkan, ölümle sonuçlanabilen akut, epizootik bir bakteriyel hastalıktır. Çalışmamızda laboratuvar deney hayvanları merkezinde yetiştirilen fare ve ratlardan alınan dışkı örneklerinde, PCR ile C. piliforme araştırıldı. Farklı yaşlardaki toplam 53 fare dışkısının 44'ünde (%83,01) C. piliforme tespit edildi. Pozitif bulunan farelerin 28'i (%73,6) dişi, 16'sı (%88,8) erkekti. Farklı yaşlardaki toplam 44 ratın dışkısının 40'ında (%90,9) C. piliforme tespit edildi. Pozitif bulunan sıçanların 25'i (%96,1) dişi, 15'i (%83,3) erkekti. Tyzzer’z Hastalığı’nın etkeni olan C. piliforme, klasik PCR yöntemiyle incelenen fare ve ratlarda yüksek oranda tespit edildi. Cinsiyet olarak değerlendirildiğinde, Tyzerr’s Hastalığı 40 adet pozitif bulunan farelerin 28’i (%73.6) dişi, 16’sı (%88.8) erkek; ratların ise 25’i (%96.1) dişi iken, 15’i (%83.3) erkek idi. Sonuç olarak C. piliforme’nin neden olduğu Tyzerr’s Hastalığı, klasik PCR ile fare ve ratlarda yüksek oranda tespit edildi. Yaş olarak değerlendirme yapıldığında hastalık farelerde 4, 5 ve 8. yaşlarda, ratlarda ise 4, 5, 6 ve 7. yaşlarda daha yüksek saptandı. Analizlerin güvenilirliği ve laboratuvar hayvanlarının yetiştirildiği merkezlerde, diğer hayvanlara bulaşma riskinin yüksek olması nedeniyle, Tyzzer’z Hastalığı’nın kontrol edilmesi ve hastalığın önlenmesi için gerekli önlemlerin alınması gerekmektedir.

Keywords

Clostridium piliforme, PCR, rat, Tyzeer's Hastalığı

Ethical Statement

This study was approved by the Veterinary Control Central Research Institute Directorate, Local Ethical Committee Decision date and no. 08.11.2022/43.

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