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Comparison of microscopy and PCR in detection of Mycobacterium tuberculosis

Year 2014, , 141 - 144, 01.12.2014
https://doi.org/10.5799/ahinjs.02.2014.04.0157

Abstract

Objective: Tuberculosis (TB) one of the most important major causes of mortality and morbidity around the world. Early detection of TB affords to prevent transmission of TB and better treatment options. The aim of this study is to evaluate sensitivity of fluorescent, Ziehl Neelsen microscopy and Polymerase Chain Reaction (PCR) method. Methods: Fifty samples of clinically suspected cases of pulmonary tuberculosis were collected and processed for Myco­bacteria by Fluorescent and ZN staining. Negative samples by staining methods were processed by PCR method using primer IS6110 of M. tuberculosis complex. Results: 33/50 (66.0%) samples were positive by Fluorescent and 23/50 (46%) were positive by ZN method. All micro­scopically negative samples (n=17) were processed to PCR and only seven of samples (41.1%) showed positive results. Conclusion: We concluded that PCR is best but it required lot of investment.

References

  • Park K. Tuberculosis, In: Park’s Textbook of Preventive and Social Medicine, 15th edition. Banarasidas Bhanot Publish- ers, 1997, 138-151.
  • WHO Global Tuberculosis Report-2012. www.who.int/iris/bitstr eam/10665/75938/1/9789241564502_eng.pdf
  • Sandhu G K. Tuberculosis: Current Situation, Challenges and Overview of its Control Programs in India J Global Infect Dis 2011;3(2):143-150.
  • Sachdeva KS, Kumar A, Dewan P, et al. New version of Re- vised National Tuberculosis Control Program (RNTCP): Uni- versal Access-“Reaching the Unreached”. Ind J Med Res 2012;135:690-694.
  • Saroj H, Nita P, Malhotra B, et al. Comparison of Zeihl Neelsen and Auramine-O-Staining Methods on Direct and Concen- tration Smears in Clinical Specimens. Ind J Tuberculosis 2011;58(2):51-53.
  • Kocagoz T, Maz E, Ozkara E, et al. Detection of Mycobacte- rium tuberculosis in sputum samples by Polymerase Chain Reaction using a simplified procedure. J Clin Microbiol 1993;31(6):1435-1438.
  • Somoskovi A, Hotaling JE, Lessons from a proficiency testing event for acid fast microscopy. Chest 2001;120(1):250-257.
  • Kansal R, Agarwal C, Madan M, Asthana AK. Rapid diagnosis of extrapulmonary tuberculosis by nested polymerase chain reaction (nPCR) based on IS 6110 insertion sequence of My- cobacterium tuberculosis. Asian J Microbiol, Biotech Env Sc 2013;15(3):517-520
  • Hendry C, Dionne K, Hedgepeth A, et al. Evaluation of a Rapid Fluorescent Staining Method for Detection of Mycobacteria in Clinical Specimens. J Clin Microbiol 2009; 47(4):1206- 1208.

Comparison of microscopy and PCR in detection of Mycobacterium tuberculosis

Year 2014, , 141 - 144, 01.12.2014
https://doi.org/10.5799/ahinjs.02.2014.04.0157

Abstract

Giriş: Tüberküloz dünyanın her yerinde hala önemli bir mortalite ve morbidite nedenidir. TB’nin erken tanınması daha iyi tedavi sonuçları ve MDR-TB’nin daha az bulaşmasını sağlar. Bu çalışmanın amacı flöresan boyama, Ziehl-Neelson mikroskobi ve PCR metotlarının duyarlılığını araştırmaktır.Yöntemler: Klinik olarak akciğer tüberküloz şüphesi olan vakalardan 50 örnek toplandı ve mikobakteriler için floresan ve ZN boyalarla değerlendirildi. Boyama metotları ile negatif olan örnekler M. tuberculosis complex IS6110 primeri kullanılarak PCR metodu ile değerlendirildi. Bulgular: Elli örnekten 33’ü (% 66,0) floresan ile ve 23’ü (% 46,0) ZN ile pozitifti. Mikroskobik olarak negative olan bütün örnekler (n=17) PCR ile çalışıldı ve sadece yedi örnek (% 41,1) pozitif bulundu. Sonuç: PCR en iyi metot olarak görülmektedir ancak ciddi altyapı yatırımı gerektirmektedir

References

  • Park K. Tuberculosis, In: Park’s Textbook of Preventive and Social Medicine, 15th edition. Banarasidas Bhanot Publish- ers, 1997, 138-151.
  • WHO Global Tuberculosis Report-2012. www.who.int/iris/bitstr eam/10665/75938/1/9789241564502_eng.pdf
  • Sandhu G K. Tuberculosis: Current Situation, Challenges and Overview of its Control Programs in India J Global Infect Dis 2011;3(2):143-150.
  • Sachdeva KS, Kumar A, Dewan P, et al. New version of Re- vised National Tuberculosis Control Program (RNTCP): Uni- versal Access-“Reaching the Unreached”. Ind J Med Res 2012;135:690-694.
  • Saroj H, Nita P, Malhotra B, et al. Comparison of Zeihl Neelsen and Auramine-O-Staining Methods on Direct and Concen- tration Smears in Clinical Specimens. Ind J Tuberculosis 2011;58(2):51-53.
  • Kocagoz T, Maz E, Ozkara E, et al. Detection of Mycobacte- rium tuberculosis in sputum samples by Polymerase Chain Reaction using a simplified procedure. J Clin Microbiol 1993;31(6):1435-1438.
  • Somoskovi A, Hotaling JE, Lessons from a proficiency testing event for acid fast microscopy. Chest 2001;120(1):250-257.
  • Kansal R, Agarwal C, Madan M, Asthana AK. Rapid diagnosis of extrapulmonary tuberculosis by nested polymerase chain reaction (nPCR) based on IS 6110 insertion sequence of My- cobacterium tuberculosis. Asian J Microbiol, Biotech Env Sc 2013;15(3):517-520
  • Hendry C, Dionne K, Hedgepeth A, et al. Evaluation of a Rapid Fluorescent Staining Method for Detection of Mycobacteria in Clinical Specimens. J Clin Microbiol 2009; 47(4):1206- 1208.
There are 9 citations in total.

Details

Primary Language English
Journal Section ART
Authors

Pooja Saxena This is me

Ashish K. Asthana This is me

Molly Madan This is me

Publication Date December 1, 2014
Published in Issue Year 2014

Cite

APA Saxena, P., Asthana, A. K., & Madan, M. (2014). Comparison of microscopy and PCR in detection of Mycobacterium tuberculosis. Journal of Microbiology and Infectious Diseases, 4(04), 141-144. https://doi.org/10.5799/ahinjs.02.2014.04.0157
AMA Saxena P, Asthana AK, Madan M. Comparison of microscopy and PCR in detection of Mycobacterium tuberculosis. J Microbil Infect Dis. December 2014;4(04):141-144. doi:10.5799/ahinjs.02.2014.04.0157
Chicago Saxena, Pooja, Ashish K. Asthana, and Molly Madan. “Comparison of Microscopy and PCR in Detection of Mycobacterium Tuberculosis”. Journal of Microbiology and Infectious Diseases 4, no. 04 (December 2014): 141-44. https://doi.org/10.5799/ahinjs.02.2014.04.0157.
EndNote Saxena P, Asthana AK, Madan M (December 1, 2014) Comparison of microscopy and PCR in detection of Mycobacterium tuberculosis. Journal of Microbiology and Infectious Diseases 4 04 141–144.
IEEE P. Saxena, A. K. Asthana, and M. Madan, “Comparison of microscopy and PCR in detection of Mycobacterium tuberculosis”, J Microbil Infect Dis, vol. 4, no. 04, pp. 141–144, 2014, doi: 10.5799/ahinjs.02.2014.04.0157.
ISNAD Saxena, Pooja et al. “Comparison of Microscopy and PCR in Detection of Mycobacterium Tuberculosis”. Journal of Microbiology and Infectious Diseases 4/04 (December 2014), 141-144. https://doi.org/10.5799/ahinjs.02.2014.04.0157.
JAMA Saxena P, Asthana AK, Madan M. Comparison of microscopy and PCR in detection of Mycobacterium tuberculosis. J Microbil Infect Dis. 2014;4:141–144.
MLA Saxena, Pooja et al. “Comparison of Microscopy and PCR in Detection of Mycobacterium Tuberculosis”. Journal of Microbiology and Infectious Diseases, vol. 4, no. 04, 2014, pp. 141-4, doi:10.5799/ahinjs.02.2014.04.0157.
Vancouver Saxena P, Asthana AK, Madan M. Comparison of microscopy and PCR in detection of Mycobacterium tuberculosis. J Microbil Infect Dis. 2014;4(04):141-4.