Molecular epidemiology of extended-spectrum beta-lactamase-producing Escherichia coli

Abstract

Objectives: E. coli O25b-ST131 has disseminated worldwide in hospitals and the community. The objective of this study was to determine the extent to which E. coli O25b-ST131 accounts for extended-spectrum beta-lactamase (ESBL)-producing E. coli from clinical samples from all sources in this region. Methods: Between January and June 2010 ESBL-producing E. coli were collected from 94 routine samples including 47 from residents of 25 nursing homes, 15 categorized as hospital acquired and 32 others. PCR was performed for detection of blaCTX-M, blaOXA-1, blaTEM, blaSHV and for the identification of members of the E. coli O25b:ST131 clonal group. PFGE was carried out using XbaI in accordance with PulseNet protocols. Results: The majority (97%) of isolates harbored a blaCTX-M gene. E. coli O25b-ST131 accounted for 87% of all ESBL-producing E. coli and for 96% of isolates from nursing home residents. Conclusion: The E. coli O25b-ST131 clonal group predominated in the collection of ESBL-producing E. coli, particularly in nursing home isolates.

Keywords

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Molecular epidemiology of extended-spectrum beta-lactamase-producing Escherichia coli

Abstract

Amaç: Escherichia coli O25b-ST131 dünyanın her tarafında hastanelerde ve toplumda yaygındır. Bu çalışmanın amacı bu bölgedeki farklı kaynaklardan alınan klinik örneklerde genişlemiş spektrumlu beta-laktamaz üreten E. coli suşları arasında E. coli O25b-ST131 yaygınlığını belirlemektir.Yöntemler: Ocak-Haziran 2010 arasında toplam olarak 25 bakımevinden 47 suş, hastane kaynaklı 15 suş ve diğer kaynaklardan 32 suş olmak üzere toplam 94 ESBL üreten E. coli suşu klinik örneklerden toplandı. PCR kullanılarak blaCTX-M, blaOXA-1, blaTEM, blaSHV ve O25b:ST131 klonal grubu üyeleri tanımlandı. PFGE tiplendirmesi PulseNet protokolüne göre XbaI kullanılarak yapıldı. Bulgular: Suşların büyük çoğunluğu (% 97) blaCTX-M geni taşımaktaydı. Bütün ESBL üreten E. coli suşlarından % 87’si ve bakımevlerinden gelen suşlardan % 96’sı E. coli O25b-ST131 idi. Sonuç: E. coli O25b-ST131 klonal grubu bakımevi kaynaklı olanlarda daha belirgin olmak üzere toplanan ESBL üreten E. coli suşları arasında baskındı

Keywords

• Escherichia coli, O25b-ST131, Direnç, Sağlık hizmeti, Bakımevi

References

1. Johnson JR N-CM-H, DebRoy C, Castanheira M, et al. Com- parison of Escherichia coli ST131 pulsotypes, by epidemio- logic traits, 1967–2009. Emerg Infect Dis 2012 Apr;18:598- 607.
2. Banerjee R, Johnston B, Lohse C, Porter S.B, Clabots C and Johnson J.R. Escherichia coli Sequence Type 131 Is a Domi- nant, Antimicrobial-Resistant Clonal Group Associated with Healthcare and Elderly Hosts. Infect Control Hosp Epidemiol 2013;34.
3. Rooney PJ, O’Leary MC, Loughrey AC, et al. Nursing homes as a reservoir of extended-spectrum β-lactamase (ESBL)- producing ciprofloxacin-resistant Escherichia coli. J Antimi- crob Chemother 2009;64:635-641.
4. Rogers BA, Sidjabat HE, Paterson DL. Escherichia coli O25b- ST131: a pandemic, multiresistant, community-associated strain. J Antimicrob Chemother 2011;66:1-14.
5. Woodford N, Carattoli A, Karisik E, et al. Complete Nucleotide Sequences of Plasmids pEK204, pEK499, and pEK516, En- coding CTX-M Enzymes in Three Major Escherichia coli Lin- eages from the United Kingdom, All Belonging to the Interna- tional O25:H4-ST131 Clone. Antimicrob Agents Chemother 2009;53:4472-4482.
6. Burke L, Humphreys H, Fitzgerald-Hughes D. The revolving door between hospital and community: extended-spectrum beta-lactamase-producing Escherichia coli in Dublin. J Hosp Infect 2012;81:192-198.
7. Clermont O, Dhanji H, Upton M, et al. Rapid detection of the O25b-ST131 clone of Escherichia coli encompassing the CTX-M-15-producing strains. J Antimicrob Chemother 2009; 64:274-277.
8. Woodford N, Fagan EJ, Ellington MJ. Multiplex PCR for rapid detection of genes encoding CTX-M extended-spectrum β-lactamases. J Antimicrob Chemother 2006;57:154-155.

9. Dallenne C, Da Costa A, Decré D, et al. Development of a set of multiplex PCR assays for the detection of genes encoding important β-lactamases in Enterobacteriaceae. J Antimicrob Chemother 2010;65:490-495.
10. Swaminathan B, T. J. Barrett, S. B. Hunter, and R. V. Tauxe. PulseNet: the molecular subtyping network for foodborne bacterial disease surveillance, United States. Emerg Infect Dis 2001;7:382–389.
11. Central Statistics Office. Profile 2 – Older and Younger. http://www.cso.ie/en/media/csoie/census/documents/cen- sus2011profile2/Profile2_Older_and_Younger_Entire_Docu- ment.pdf. 2012.