Methicillin resistant Staphylococcus aureus nasal colonization among secondary school students at Duhok City-Iraq

Abstract

Objective: Methicillin-resistant Staphylococcus aureus (MRSA) widely distributed in hospitals around the world. There is strong relationship between disease development and S. aureus nasal carriage. The aim of this study was to evaluate the prevalence and epidemiology of nasal colonization with S. aureus and MRSA in the community of Duhok city, Iraq. Methods: 489 students aged 16 to18 years were included. Nasal swab samples were collected followed by antimicrobial susceptibility test. MRSA isolates were selected and investigated for the mecA gene. Also the prevalence of Panton-Valentine Leukocidin (PVL) gene was also studied. Results: A total of 90 (18.4%) out of 489 (18.4%) of the students were found to be colonized by S. aureus. Only 10 (2.04%) of the students were found to be MRSA carrier. All MRSA isolates were sensitive to Vancomycin. PLV gene was detected in one MRSA strain. Conclusion: This is the first study investigating S. aureus colonization in students in the Duhok city. Nasal carriage of S. aureus and MRSA is comparable with reports from elsewhere. Fortunately, all trains included in our study were sensitive to vancomycin. Further research is needed to examine the SCCmec elements and the evolution of MRSA over the time.

Keywords

• Methicillin-resistant Staphylococcus aureus, Staphylococcus aureus, community acquired-MRSA, Vancomycin, Iraq Irak\'ın Du

Methicillin resistant Staphylococcus aureus nasal colonization among secondary school students at Duhok City-Iraq

Öz

Amaç: Metisiline dirençli Staphylococcus aureus (MRSA) tüm dünyada hastanelerde ve yoğun bakım ünitelerinde yaygın şekilde bulunmaktadır. Burunda taşıyıcılık ile MRSA’ya bağlı hastalık gelişmesi arasında belirgin bir ilişki bulunmuştur. Bu çalışmanın amacı Irak’ın Duhok şehrinde toplumda S. aureus ve MRSA burun kolonizasyonunun prevalansını ve epidemiyolojik özelliklerini belirlemektir. Yöntemler: Çalışmaya yaşları 16-18 arasında değişen 489 öğrenci dahil edildi. Burun sürüntü kültürleri alındı ve antimikrobiyal duyarlılık testleri yapıldı. MRSA izolatları seçilerek bunlarda mecA geninin varlığı araştırıldı. Yanısıra bu izolatlarda Panton-Valentin Lökosidin (PVL) geninin varlığı da araştırıldı.Bulgular: Çalışmayan alınan 489 öğrenciden 90’ında (% 18,4) S. aureus ile kolonizasyon saptandı. Bunlardan sadece 10’unda (% 2,04) MRSA taşıyıcılığı belirlendi. Tüm MRSA izolatları vankomisine duyarlı idi. PLV geni sadece bir MRSA izolatında belirlendi.Sonuçlar: Bu çalışma Duhok şehrinde öğrencilerde S. aureus kolonizasyonunu araştıran ilk çalışmadır. Burunda S. aureus taşıyıcılığı ve MRSA oranları farklı merkezlerle benzer oranda bulunmuştur. SCCmec öğelerini araştırmak ve zaman içerisinde MRSA yayılımını araştırmak için ileri çalışmalara ihtiyaç olduğu görülmektedir

Anahtar Kelimeler

• Metisiline dirençli Staphylococcus aureus, MRSA, Staphylococcus aureus, toplumda kazanılmış MRSA, Vancomisin, Irak

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