In-vitro susceptibility of multiple drug resistant Pseudomonas aeruginosa to organic acids

Abstract

Objective: Pseudomonas aeruginosa is a classic opportunistic pathogen with innate resistance to many antibiotics and disinfectants. Resistance to antimicrobial agents makes it the most noxious organism to eliminate from infection site. In view of its antimicrobial resistance, an attempt was made to study its susceptibility to various organic acids. Methods: Seven clinical isolates of P.aeruginosa resistant to multiple antibiotics were subjected to in vitro susceptibility to various organic acids by broth dilution method to find out susceptibility to various organic acids. Results: The isolates of P.aeruginosa resistant to 14 antimicrobials were found susceptible to one percent oxalic acid and trichloroacetic acid, two percent lactic acid and citric acid, and three percent acetic acid. It is interesting to note that strains resistant to multiple antibiotics were also found susceptible to organic acids. Oxalic acid and trichloroacetic acid were found highly effective. Conclusions: Clinical use of oxalic acid, trichloroacetic acid and lactic acid as topical agents to treat superficial pseu­domonal infections caused by difficult strains of P.aeruginosa may be recommended after confirmation of their toxicity and in vivo efficacy in animal models.

Keywords

• Pseudomonas aeruginosa

In-vitro susceptibility of multiple drug resistant Pseudomonas aeruginosa to organic acids

Öz

Amaç: Pseudomonas aeruginosa fırsatçı bir patojen olup birçok antibiyotiğe ve dezenfektana doğal dirençlidir. Antimikrobiyal direnci nedeniyle bu etkenleri elimine etmek zordur. Antimikrobiyal direnci göz önüne alınarak bakterilerin çeşitli organik asitlere duyarlılığının araştırılması amaçlanmıştır.Yöntemler: Toplam yedi dirençli P. aeruginosa izolatının çeşitli organik asitlere karşı etkinliği sıvı mikrodilüsyon tekniği ile araştırıldı.Bulgular: Farklı 14 antibiyotiğe dirençli P. aeruginosa izolatının %1 oranında oksalik asit ve trikloroasetik asite, %2 laktik asit ve sitrik aside %3 oranında asetik asite hassas olduğu gözlendi. İlginç olan nokta ise birçok antibiyotiğe dirençli olan bu kökenler organik asitlere hassastı. Oksalik asid ve trikloroasetik asit oldukça etkin bulundu.Sonuç: Bulgularımız toksisite ve invivo etkinlik testleri ile doğrulanmasının ardından oksalik asit, trikloro asetik asit ve laktik asit tedavisi güç P. aeruginosa enfeksiyonlarının topikal tedavisinde kullanılması önerilebilir

Anahtar Kelimeler

• Pseudomonas aeruginosa, çoklu antibiyotik direnci, organik asidlere duyarlılık

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