Molecular characterization and antibiogram of Listeria monocytogenes isolated from chicken and mutton of retail markets

Abstract

Objective: To isolate Listeria monocytogenes from chicken and mutton meat sold at retail outlets and to characterize isolates for virulence determinants. Methods: Listeria monocytogenes was isolated from chicken and mutton meat samples using ISO: 11290 method. Multiplex PCR was performed for the detection of virulence associated genes. Results: Out of 198 retail meat samples (72 chicken and 126 mutton) analyzed, Listeria monocytogenes was isolated from 4.5% (8.3% chicken and 2.3% mutton) samples. All the 9 isolates (6 from chicken and 3 from mutton) belonged to 1/2a servar and carried virulence genes viz. haemolysin (hlyA), phosphatidylinositol phospholipase (plcA), actin polymerization protein (actA) and invasive associative protein p60 (Iap). Conclusion: L. monocytogenes is an organism of food safety and public health significance, its recovery from the meat sold at retail outlets indicated breach in the quality assurance. J Microbiol Infect Dis 2016;6(2): 65-68   

Keywords

• Listeria monocytogenes,chicken,mutton,virulence marker

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