Thiol-disulphide homeostasis in patients with surgical site infections

Abstract

Objectives: Introduction: In infectious diseases, various inflammatory cells are active and reactive oxygen species are produced to fight against intra cellular and extra cellular microorganisms. This leads to an increase in potential antioxidant capacity and free radical production. Thiol is an important antioxidant. Thiols enter oxidation reactions with oxidative molecules and it form disulphide bonds. The aim of this study was to evaluate the dynamic thiol/disulphide homeostasis in patients with surgical site infection (SSI). Methods: Sixty-eight patients with SSI and 66 healthy persons (control group) were included in this study. Complete blood count, biochemistry, erythrocyte sedimentation rate, C-reactive protein values and thiol levels were studied in blood of the patients at 0th day and 10th day. The blood thiol disulphide homeostasis was analysed using a new automated method developed by Erel and Neselioglu. Results: Native thiol, total thiol, albumin levels and native thiol/total thiol ratio were found significantly lower in the blood samples at day 0th compared to 10th day. There was a statistically significant difference between the patient group and the control group in IMA, native thiol, total thiol, albumin, disulphide levels, disulphide/native thiol, disulphide/total thiol and native thiol/total thiol ratios Conclusions: The thiol-disulphide balance is impaired in SSI. The elevated disulphide/total thiol ratio and disulphide/native thiol ratio, and reduced native thiol/total thiol ratio indicate increased oxidation in SSI. There is also a strong association between CRP, ESH and thiol-disulphide parameters. Thiol-disulphide homeostasis may potentially be of benefit in inflammatory response. J Microbiol Infect Dis 2020; 10(3):160-166.

Keywords

• SSI
• thiol-disulphide homeostasis
• inflammatory response

References

1. 1.National Healthcare Safety Network, Centers for Disease Control and Prevention. Surgical site infection (SSI) event. http://www.cdc.gov/nhsn/pdfs/pscmanual/9pscssicurrent.pdf. Published January 2020. Accessed January 01, 2020.
2. 2. Esen R, Aslan M, Kucukoglu ME, et al. Serum paraoxonase activity, total thiols levels, and oxidative status in patients with acute brucellosis. Wien Klin Wochenschr 2015;127(11–12):427–433.
3. 3. Dundaroz R, Erenberk U, Turel O, Demir AD, Ozkaya E, Erel O. Oxidative and antioxidative status of children with acute bronchiolitis. J Pediatr (Rio J) 2013;89(4):407–411.
4. 4. Cadenas E. Biochemistry of oxygen toxicity. Annu Rev Biochem 1989; 58:79-110.
5. 5. Kuo LM, Kuo CY, Lin CY, Hung MF, Shen JJ, Hwang TL. Intra cellular glutathione depletion by oridonin leads to apoptosis in hepatic stellate cells. Molecules 2014; 19(3):3327-3344.
6. 6. Prabhu A, Sarcar B, Kahali S, et al. Cysteine catabolism: A novel metabolic pathway contributing to glioblastoma growth. Cancer Res. 2014; 74(3):787-796.
7. 7. Roy D, Quiles J, Gaze D, Collinson P, Kaski J, Baxter GF. Role of reactive oxygen species on the formation of the novel diagnostic marker ischaemia modified albumin. Heart 2006; 92(1): 113-114.
8. 8. Gidenne S, Ceppa F, Fontan E, Perrier F, Burnat P. Analytical performance of the Albumin Cobalt Binding (ACB) test on the Cobas MIRA Plus analyzer. Clin Chem Lab Med 2004; 42(4):455-461.

9. 9. Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem 2014; 47(18):326-332.
10. 10. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007; 39:44-84.
11. 11. Kundi H, Ates I, Kiziltunc E, et al. A novel oxidative stress marker in acute myocardial infarction; thiol/disulfide homeostasis. Am J Emerg Med 2015; 33:1567-1571.
12. 12. Tufan ZK, Hasanoglu I, Kolgelier S, et al. A retrospective controlled study of thiol disulfide homeostasis as a novel marker in Crimean Congo hemorrhagic fever. Redox Rep 2016; 21:1-5.
13. 13. Kara SS, Erel O, Demirdag TB, Yayla BCC, Gulhan B, Neselioglu S. Alteration of thiol-disulfide homeostasis in acute tonsillopharyngitis. Redox Rep 2016; 20:1-5.
14. 14. Kolgelier S, Ergin M, Demir LF, et al. Impaired thiol-disulfide balance in acute brucellosis. Jpn J Infect Dis 2017;70:258-262.
15. 15. Ozyazici S, Karateke F, Turan U, et al. Novel Oxidative Stress Mediator in Acute Appendicitis: Thiol/Disulphide Homeostasis. Mediators Inflamm 2016; 2016:6761050.
16. 16. Bar-Or D, Lau E, Winkler J. A novel assay for cobalt-albumin binding and its potential as a marker for myocardial ischemia- a preliminary report. J Emerg Med 2000;19(4): 311–315.
17. 17. Cakir M, Karahan S, Mentese A, et al. Ischemia- Modified Albumin Levels in Children with Chronic Liver Disease. Gut Liver 2012; 6(1): 92-97.
18. 18. Turedi S, Patan T, Gunduz A, et al. Ischemia-modified albumin in the diagnosis of pulmonary embolism: an experimental study. Am J Emerg Med 2009; 27(6):635-640.
19. 19. Turedi S, Cinar O, Yavuz I, et al. Differences in ischemia modified albumin levels between end stage renal disease patients and the normal population. J Nephrol 2010; 23(3):335–340.
20. 20. Gunduz A, Turedi S, Mentese A, et al. Ischemia-modified albumin levels in cerebrovascular accidents. Am J Emerg Med 2008; 26(8):874–878.
21. 21. Ma S, Xu W, Wei C. Role of ischemia-modified albumin and total homocysteine in estimating symptomatic lacunar infarction in type 2 diabetic patients. Clin Biochem 2011; 44(16):1299–1303.
22. 22. Shao-gang M, Yao J, Wen H, Feng B, Wen X, Wei-nan Y. Evaluation of Ischemia Modified Albumin and C-Reactive Protein in Type 2 Diabetics with and without Ketosis. Biomark Insights 2012; 7:19-26.
23. 23. Can M, Demirtas S, Rolat O, Yıldız A. Evaluation of effects of ischemia on the albumin cobalt binding (ACB) assay in patients exposed to trauma. Am J Emerg Med 2006; 23(7):537-539.
24. 24. Caglar G, Oztas E, Karadag D, Pabuccu R, Demirtas S. Ischemia Modified Albumin and cardiovascular risk markers in polycystic ovary syndrome with or without insulin resistance. Fertil Steril 2011; 95(1): 310-313.