Pathogenic Vibrio Strains Isolated from Human Stool and Water Samples from Western Kenya

Year 2016, Volume: 6 Issue: 1, 1 - 7, 01.03.2016

Roselida Achieng Owuor

https://doi.org/10.5799/jmid.328759

Abstract

Objective: Investigate the type of pathogenic Vibrio strains from water and stool samples collected from Migori, SonduMiriu,
Nyando and Yala regions in Western Kenya.
Methods: A total of 811 samples (596 water and 215 stool samples) were collected during the study periods of May
to December 2013 and August to September 2014. Pathogenic Vibrio strains were identified through culturing in TCBS
Agar, followed by oxidation, string and serological (polyvalent) tests, respectively. The PCR analysis was done using
combined primers targeting Vibrionaceae 16SrRNA and species specific primers for V. vulnificus and V. cholerae.
Results: The results showed the presence of V. vulnificus and V. cholerae. However, V. parahaemolyticus was not found
in any of the samples. The PCR results for 16SrRNA, Vib 1, and Vib 2 showed polymorphism in the genes, this was an
indication of cross combination of genes from more than one strain in one isolate.
Conclusion: The study showed the presence of V. cholerae (Ogawa and Inaba) in water and human stool samples. Type
B V. vulnificus was detected in the water sample collected from River Migori. This information is of essence in controlling
and managing cholera in the western part of Kenya. J Microbiol Infect Dis 2016;6(1): 1-7

Keywords

V. vulnificus, V. parahaemolyticus, PCR, V. cholerae

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