Year 2019, Volume 09 , Issue 03, Pages 125 - 128 2019-09-15

A Study of Inducible Clindamycin Resistance among Staphylococcus aureus Skin and Soft Tissue Infections in A Tertiary Care Hospital

Abirami Lakshmy Jayachandiran [1] , Balan Kandasamy [2] , Sangeetha Vilwanatha [3] , Sheila Doris Devami T. [4] , Vanitha Devi E. [5] , Vandhitha Muralidharan [6]


ABSTRACT

Objectives: Drug resistant phenotypes like MRSA are difficult to treat requiring higher group of antibiotics. Topical agents like clindamycin can be used for the therapy of MRSA. The knowledge of prevalence of inducible clindamycin resistance phenotype is essential to prevent treatment failure.

Methods: A total of 204 staphylococcal isolates obtained from skin and soft tissue infections and MRSA was detected by Cefoxitin disc diffusion method and detection of Mec A gene by Polymerase chain reaction (PCR). Antibiotic susceptibility testing was performed by Kirby Baeur disc diffusion method. The Erythromycin resistant isolates were tested for D test. The differences in antibiotic susceptibility pattern between MRSA and MSSA was compared by Chi Square test using Graph pad Quick Calcs software and p value less than 0.05 was considered as significant

Results: Out of the 204 Staphylococcus aureus isolates, 48 (23.5%) were identified as MRSA by Cefoxitin disc diffusion method. All these 48(23.5%) of isolates were also positive for Mec A gene by PCR. Inducible clindamycin resistance (iMLSB resistance phenotype) was observed among 24(11.7%) of the isolates. MRSA showed comparatively lesser susceptibility than MSSA (p ≤0.05). Among the MRSA inducible clindamycin resistance was seen among 11 (22.9%).

Conclusions: Emergence of drug resistance warrants antibiotic susceptibility testing for all the isolates in the laboratory. Cefoxitin disc diffusion method can be used in resource constraint laboratory where PCR facilities are not available. Inducible clindamycin resistance phenotype (iMLS phenotype) must be checked for all isolates showing erythromycin resistance to prevent treatment failure. J Microbiol Infect Dis 2019; 9(3):125-128.

Methicillin resistant Staphylococcus aureus (MRSA), iMLSB resistance phenotype
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Primary Language en
Journal Section Research Article
Authors

Author: Abirami Lakshmy Jayachandiran (Primary Author)
Institution: Karpaga Vinayaga Institute of Medical Sciences and Research Centre, Madurantakam, Tamilnadu, India
Country: India


Author: Balan Kandasamy

Author: Sangeetha Vilwanatha

Author: Sheila Doris Devami T.

Author: Vanitha Devi E.

Author: Vandhitha Muralidharan

Dates

Publication Date : September 15, 2019

Bibtex @research article { jmid614241, journal = {Journal of Microbiology and Infectious Diseases}, issn = {2146-3158}, eissn = {2146-9369}, address = {}, publisher = {Sağlık Araştırmaları Derneği}, year = {2019}, volume = {09}, pages = {125 - 128}, doi = {10.5799/jmid.614241}, title = {A Study of Inducible Clindamycin Resistance among Staphylococcus aureus Skin and Soft Tissue Infections in A Tertiary Care Hospital}, key = {cite}, author = {Jayachandiran, Abirami Lakshmy and Kandasamy, Balan and Vilwanatha, Sangeetha and T., Sheila Doris Devami and E., Vanitha Devi and Muralidharan, Vandhitha} }
APA Jayachandiran, A , Kandasamy, B , Vilwanatha, S , T., S , E., V , Muralidharan, V . (2019). A Study of Inducible Clindamycin Resistance among Staphylococcus aureus Skin and Soft Tissue Infections in A Tertiary Care Hospital. Journal of Microbiology and Infectious Diseases , 09 (03) , 125-128 . DOI: 10.5799/jmid.614241
MLA Jayachandiran, A , Kandasamy, B , Vilwanatha, S , T., S , E., V , Muralidharan, V . "A Study of Inducible Clindamycin Resistance among Staphylococcus aureus Skin and Soft Tissue Infections in A Tertiary Care Hospital". Journal of Microbiology and Infectious Diseases 09 (2019 ): 125-128 <https://dergipark.org.tr/en/pub/jmid/issue/48485/614241>
Chicago Jayachandiran, A , Kandasamy, B , Vilwanatha, S , T., S , E., V , Muralidharan, V . "A Study of Inducible Clindamycin Resistance among Staphylococcus aureus Skin and Soft Tissue Infections in A Tertiary Care Hospital". Journal of Microbiology and Infectious Diseases 09 (2019 ): 125-128
RIS TY - JOUR T1 - A Study of Inducible Clindamycin Resistance among Staphylococcus aureus Skin and Soft Tissue Infections in A Tertiary Care Hospital AU - Abirami Lakshmy Jayachandiran , Balan Kandasamy , Sangeetha Vilwanatha , Sheila Doris Devami T. , Vanitha Devi E. , Vandhitha Muralidharan Y1 - 2019 PY - 2019 N1 - doi: 10.5799/jmid.614241 DO - 10.5799/jmid.614241 T2 - Journal of Microbiology and Infectious Diseases JF - Journal JO - JOR SP - 125 EP - 128 VL - 09 IS - 03 SN - 2146-3158-2146-9369 M3 - doi: 10.5799/jmid.614241 UR - https://doi.org/10.5799/jmid.614241 Y2 - 2019 ER -
EndNote %0 Journal of Microbiology and Infectious Diseases A Study of Inducible Clindamycin Resistance among Staphylococcus aureus Skin and Soft Tissue Infections in A Tertiary Care Hospital %A Abirami Lakshmy Jayachandiran , Balan Kandasamy , Sangeetha Vilwanatha , Sheila Doris Devami T. , Vanitha Devi E. , Vandhitha Muralidharan %T A Study of Inducible Clindamycin Resistance among Staphylococcus aureus Skin and Soft Tissue Infections in A Tertiary Care Hospital %D 2019 %J Journal of Microbiology and Infectious Diseases %P 2146-3158-2146-9369 %V 09 %N 03 %R doi: 10.5799/jmid.614241 %U 10.5799/jmid.614241
ISNAD Jayachandiran, Abirami Lakshmy , Kandasamy, Balan , Vilwanatha, Sangeetha , T., Sheila Doris Devami , E., Vanitha Devi , Muralidharan, Vandhitha . "A Study of Inducible Clindamycin Resistance among Staphylococcus aureus Skin and Soft Tissue Infections in A Tertiary Care Hospital". Journal of Microbiology and Infectious Diseases 09 / 03 (September 2019): 125-128 . https://doi.org/10.5799/jmid.614241
AMA Jayachandiran A , Kandasamy B , Vilwanatha S , T. S , E. V , Muralidharan V . A Study of Inducible Clindamycin Resistance among Staphylococcus aureus Skin and Soft Tissue Infections in A Tertiary Care Hospital. J Microbil Infect Dis. 2019; 09(03): 125-128.
Vancouver Jayachandiran A , Kandasamy B , Vilwanatha S , T. S , E. V , Muralidharan V . A Study of Inducible Clindamycin Resistance among Staphylococcus aureus Skin and Soft Tissue Infections in A Tertiary Care Hospital. Journal of Microbiology and Infectious Diseases. 2019; 09(03): 128-125.