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Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination

Year 2021, Volume: 11 Issue: 02, 88 - 94, 15.06.2021
https://doi.org/10.5799/jmid.951600

Abstract

Objectives: Echinococcosis is a cyclozoonotic disease caused by E. granulosus. The canine mainly the dog represents the definitive host and immunity plays an important role in the control of the disease. This study was aimed to detect Echinococcus granulosus B-antibody for Dot-ELISA and antigen for counter-current immune electrophoresis (CIEP) and bacterial co-agglutination assay (Co-A).
Methods: Follow-up of 23 surgical and chemotherapy patients from different hospitals of Khartoum were performed.
Results: Results: Sensitivity and specificity for Dot-ELISA was 94.4% and 100%, respectively within seven days to 6 months except for the relapse all or most of the cases were negative for the disease whereas the sensitivity of CIEP and Co-A for Echinococcus granulosus antigen in patient sera was 77.8% and 94.4% respectively with a specificity of 100% and 75% respectively. Dot-ELISA, showed high positive and negative predictive value (100%, 95.2%), respectively with CIEP (100%, 83.3%) and in Co-A (81%, 94.1%).
Conclusion: Conclusion: Therefore, detection of antigen of E. granulosus in the serum of operative, post-operative, or chemotherapeutic treated patient using CIEP and Co-A or detection of antibodies using IgG ELISA to the antigen B-rich fraction in (ELISA) assay is useful for following treated hydatid patients. J Microbiol Infect Dis 2021; 11(2):88-94.

References

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  • 2. Brunetti E, Kern P, Vuitton DA. Expert consensus for the diagnosis and treatment of cystic and alveolar Echinococcosis in humans. Acta Trop 2010;114(1) :1-16.
  • 3. Thompson, R.C.A. and McManus, D. Aetiology: parasites and life-cycles. In: Eckert, J., Gemmel, M.A., Meslin, F-X and Pawłowski, Z.S., (eds.) WHO/OIE Manual on Echinococcosis in Human and Animals: a Public Health Problem of Global Concern. World Organization for Animal Health, 2001, Paris, France, pp. 1-19.
  • 4. Force L, Torres JM, Carrillo A, et al. Evaluation of eight serological tests in the diagnosis of human Echinococcosis and follow-up. Clin Infect Dis 1992 ;15(3):473-480.
  • 5. Shariff GM, Parija SC. Counter-current immunoelectrophoresis test for serodiagnosis of hydatid disease by detection of circulating hydatid antigen. J Microbiol Methods 1991;14(2):71-76.
  • 6. Dalimiasl AAH, Madani R, Ghorbankhani D, et al . Comparative evaluation of serodiagnostic Techniques in Hydatid Cyst Disease. Arch Razi Ins 2000; 51: 85-93.
  • 7. Gottstein B. An immunoassay for the detection of circulating antigens in human Echinococcosis. Am J Trop Med Hyg 1984;33(6):1185-1191.
  • 8. Zheng GY, Zhao RL, Feng XH. Dot-immunobinding assay in the serodiagnosis of human hydatid disease. Am J Trop Med Hyg 1986;35(4):812-814.
  • 9. Romig T, Omer RA, Zeyhle E, et al. Echinococcosis in sub-Saharan Africa: emerging complexity. Vet Parasitol 2011;181(1):43-47.
  • 10. Elmahdi IE, Ali QM, Magzoub MMA, et al. Cystic Echinococcosis of livestock and humans in central Sudan. Ann Trop Med Parasitol 2004; 98(5):473-479.
  • 11. Mohammed AA, El-Malik KH. The Epidemiology of Cystic Echinococcosis in Nyala, Southern Darfur State, Sudan .Sud. J. Vet. Res 2000 16:49-53.
  • 12. Osman AMA, Aradaib IE, Ashmaig ALK, et al. Rapid detection of Echinococcus granulosus-complex and specific identification camel genotype (G6) a nested PCR. In Proceeding Echinococcus of the 22nd International Congress of Hydatiology, Athens, Greece .2007: 80-85.
  • 13. Omer RA, Dinkel A, Romig T, et al. A molecular survey of cystic Echinococcosis in Sudan. Vet Parasitol 2010; 169(3-4):340-346.
  • 14. Ibrahim K, Thomas R, Peter K, et al. A molecular survey on cystic Echinococcosis in Sinnar area, Blue Nile state (Sudan). Chin Med J 2011;124(18):2829-2833.
  • 15. National Center for Health Statistics (US) and National Center for Health Services Research, 1994. Health, United States. US Department of Health, Education, and Welfare, Public Health Service, Health Resources Administration, National Center for Health Statistics.
  • 16. Wattal CH, Malla NA, Khan IA, et al. Comparative evaluation of enzyme-linked immunosorbent assay for the diagnosis of pulmonary Echinococcosis. J Clin Microbiol 1986;24(1):41-46.
  • 17. Kanwar JR, Kaushik SP, Sawhney IM, et al. Specific antibodies in serum of patients with hydatidosis recognised by immunoblotting. J Med Microbiol 1992 ;36(1):46-51.
  • 18. Sbihi Y, Janssen D, Osuna A. Serologic recognition of hydatid cyst antigens using different purification methods. Diagn Microbiol Infect Dis 1996;24(4):205-211.
  • 19. Babba H, Messedi A, Masmoudi S, et al. Diagnosis of human hydatidosis: comparison between imagery and six serologic techniques. Am J Trop Med Hyg 1994;50(1):64-68.
  • 20. Pappas MG. Recent applications of the Dot-ELISA in immunoparasitology. Vet Parasitol 1988;29(2-3):105-129.
  • 21. Rogan MT, Craig PS, Zeyhle E, et al. Evaluation of a rapid dot-ELISA as a field test for the diagnosis of cystic hydatid disease. Trans R Soc Trop Med Hyg1991;85(6):773-777.
  • 22. Romia SA, Youssef ME, Handoussa AE, et al. Dot-ELISA as a diagnostic test in hydatid disease. J Egypt Soc Parasitol 1992;22(3):603-610.
  • 23. Parija SC. A review of some simple immunoassays in the serodiagnosis of cystic hydatid disease. Acta Trop 1998;70(1):17-24.
  • 24. Ramadan MM, El-Ameer AM, Shalash IR, et al. Diagnosis of Echinococcosis (hydatidosis) using dot-ELISA. Egypt J Hosp Med 2016 ;64(1):304-310.
  • 25. Shariff M, Parija SC. Co-agglutination (Co-A) test for circulating antigen in hydatid disease. J Med Microbiol 1993; 38(6):391-394.
Year 2021, Volume: 11 Issue: 02, 88 - 94, 15.06.2021
https://doi.org/10.5799/jmid.951600

Abstract

References

  • 1. Budke CM, Carabin H, Ndimubanzi PC, et al. A systematic review of the literature on cystic Echinococcosis frequency worldwide and its associated clinical manifestations. Am J Trop Med Hyg 2013;88(6):1011-1027.
  • 2. Brunetti E, Kern P, Vuitton DA. Expert consensus for the diagnosis and treatment of cystic and alveolar Echinococcosis in humans. Acta Trop 2010;114(1) :1-16.
  • 3. Thompson, R.C.A. and McManus, D. Aetiology: parasites and life-cycles. In: Eckert, J., Gemmel, M.A., Meslin, F-X and Pawłowski, Z.S., (eds.) WHO/OIE Manual on Echinococcosis in Human and Animals: a Public Health Problem of Global Concern. World Organization for Animal Health, 2001, Paris, France, pp. 1-19.
  • 4. Force L, Torres JM, Carrillo A, et al. Evaluation of eight serological tests in the diagnosis of human Echinococcosis and follow-up. Clin Infect Dis 1992 ;15(3):473-480.
  • 5. Shariff GM, Parija SC. Counter-current immunoelectrophoresis test for serodiagnosis of hydatid disease by detection of circulating hydatid antigen. J Microbiol Methods 1991;14(2):71-76.
  • 6. Dalimiasl AAH, Madani R, Ghorbankhani D, et al . Comparative evaluation of serodiagnostic Techniques in Hydatid Cyst Disease. Arch Razi Ins 2000; 51: 85-93.
  • 7. Gottstein B. An immunoassay for the detection of circulating antigens in human Echinococcosis. Am J Trop Med Hyg 1984;33(6):1185-1191.
  • 8. Zheng GY, Zhao RL, Feng XH. Dot-immunobinding assay in the serodiagnosis of human hydatid disease. Am J Trop Med Hyg 1986;35(4):812-814.
  • 9. Romig T, Omer RA, Zeyhle E, et al. Echinococcosis in sub-Saharan Africa: emerging complexity. Vet Parasitol 2011;181(1):43-47.
  • 10. Elmahdi IE, Ali QM, Magzoub MMA, et al. Cystic Echinococcosis of livestock and humans in central Sudan. Ann Trop Med Parasitol 2004; 98(5):473-479.
  • 11. Mohammed AA, El-Malik KH. The Epidemiology of Cystic Echinococcosis in Nyala, Southern Darfur State, Sudan .Sud. J. Vet. Res 2000 16:49-53.
  • 12. Osman AMA, Aradaib IE, Ashmaig ALK, et al. Rapid detection of Echinococcus granulosus-complex and specific identification camel genotype (G6) a nested PCR. In Proceeding Echinococcus of the 22nd International Congress of Hydatiology, Athens, Greece .2007: 80-85.
  • 13. Omer RA, Dinkel A, Romig T, et al. A molecular survey of cystic Echinococcosis in Sudan. Vet Parasitol 2010; 169(3-4):340-346.
  • 14. Ibrahim K, Thomas R, Peter K, et al. A molecular survey on cystic Echinococcosis in Sinnar area, Blue Nile state (Sudan). Chin Med J 2011;124(18):2829-2833.
  • 15. National Center for Health Statistics (US) and National Center for Health Services Research, 1994. Health, United States. US Department of Health, Education, and Welfare, Public Health Service, Health Resources Administration, National Center for Health Statistics.
  • 16. Wattal CH, Malla NA, Khan IA, et al. Comparative evaluation of enzyme-linked immunosorbent assay for the diagnosis of pulmonary Echinococcosis. J Clin Microbiol 1986;24(1):41-46.
  • 17. Kanwar JR, Kaushik SP, Sawhney IM, et al. Specific antibodies in serum of patients with hydatidosis recognised by immunoblotting. J Med Microbiol 1992 ;36(1):46-51.
  • 18. Sbihi Y, Janssen D, Osuna A. Serologic recognition of hydatid cyst antigens using different purification methods. Diagn Microbiol Infect Dis 1996;24(4):205-211.
  • 19. Babba H, Messedi A, Masmoudi S, et al. Diagnosis of human hydatidosis: comparison between imagery and six serologic techniques. Am J Trop Med Hyg 1994;50(1):64-68.
  • 20. Pappas MG. Recent applications of the Dot-ELISA in immunoparasitology. Vet Parasitol 1988;29(2-3):105-129.
  • 21. Rogan MT, Craig PS, Zeyhle E, et al. Evaluation of a rapid dot-ELISA as a field test for the diagnosis of cystic hydatid disease. Trans R Soc Trop Med Hyg1991;85(6):773-777.
  • 22. Romia SA, Youssef ME, Handoussa AE, et al. Dot-ELISA as a diagnostic test in hydatid disease. J Egypt Soc Parasitol 1992;22(3):603-610.
  • 23. Parija SC. A review of some simple immunoassays in the serodiagnosis of cystic hydatid disease. Acta Trop 1998;70(1):17-24.
  • 24. Ramadan MM, El-Ameer AM, Shalash IR, et al. Diagnosis of Echinococcosis (hydatidosis) using dot-ELISA. Egypt J Hosp Med 2016 ;64(1):304-310.
  • 25. Shariff M, Parija SC. Co-agglutination (Co-A) test for circulating antigen in hydatid disease. J Med Microbiol 1993; 38(6):391-394.
There are 25 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Research Article
Authors

Omer Hassan Siddig Elamin This is me

Mai Abdalla Alı This is me

Ibrahim Alhag Almahadi Elgezuli This is me

Mona Alrasheed Bashir This is me

Ivan Kahwa This is me

Publication Date June 15, 2021
Published in Issue Year 2021 Volume: 11 Issue: 02

Cite

APA Elamin, O. H. S., Alı, M. A., Elgezuli, I. A. A., Bashir, M. A., et al. (2021). Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination. Journal of Microbiology and Infectious Diseases, 11(02), 88-94. https://doi.org/10.5799/jmid.951600
AMA Elamin OHS, Alı MA, Elgezuli IAA, Bashir MA, Kahwa I. Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination. J Microbil Infect Dis. June 2021;11(02):88-94. doi:10.5799/jmid.951600
Chicago Elamin, Omer Hassan Siddig, Mai Abdalla Alı, Ibrahim Alhag Almahadi Elgezuli, Mona Alrasheed Bashir, and Ivan Kahwa. “Diagnosis of Pre- and Post-Treatment of Echinococcus Granulosus With Counter Current Immune Electrophoresis and Bacterial Co-Agglutination”. Journal of Microbiology and Infectious Diseases 11, no. 02 (June 2021): 88-94. https://doi.org/10.5799/jmid.951600.
EndNote Elamin OHS, Alı MA, Elgezuli IAA, Bashir MA, Kahwa I (June 1, 2021) Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination. Journal of Microbiology and Infectious Diseases 11 02 88–94.
IEEE O. H. S. Elamin, M. A. Alı, I. A. A. Elgezuli, M. A. Bashir, and I. Kahwa, “Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination”, J Microbil Infect Dis, vol. 11, no. 02, pp. 88–94, 2021, doi: 10.5799/jmid.951600.
ISNAD Elamin, Omer Hassan Siddig et al. “Diagnosis of Pre- and Post-Treatment of Echinococcus Granulosus With Counter Current Immune Electrophoresis and Bacterial Co-Agglutination”. Journal of Microbiology and Infectious Diseases 11/02 (June 2021), 88-94. https://doi.org/10.5799/jmid.951600.
JAMA Elamin OHS, Alı MA, Elgezuli IAA, Bashir MA, Kahwa I. Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination. J Microbil Infect Dis. 2021;11:88–94.
MLA Elamin, Omer Hassan Siddig et al. “Diagnosis of Pre- and Post-Treatment of Echinococcus Granulosus With Counter Current Immune Electrophoresis and Bacterial Co-Agglutination”. Journal of Microbiology and Infectious Diseases, vol. 11, no. 02, 2021, pp. 88-94, doi:10.5799/jmid.951600.
Vancouver Elamin OHS, Alı MA, Elgezuli IAA, Bashir MA, Kahwa I. Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination. J Microbil Infect Dis. 2021;11(02):88-94.