RNAemia and Clinical Outcome in COVID-19 Patients

Jyotsna Agarwal; Vikramjeet Singh; Jaya Garg; Ritu Karoli2; Swasti Tiwari; Shareh Naqvi; Anupam Das; Manodeep Sen

doi:10.5799/jmid.993839

Research Article

RNAemia and Clinical Outcome in COVID-19 Patients

Year 2021, Volume: 11 Issue: 03, 116 - 123, 15.09.2021

Jyotsna Agarwal Vikramjeet Singh Jaya Garg Ritu Karoli2 Swasti Tiwari Shareh Naqvi Anupam Das Manodeep Sen

Abstract

Objectives: Objectives: SARS-CoV-2 virus dynamics in the human body and its correlation with disease severity and biomarkers have not been elucidated. This study aims to evaluate the viral load in the blood sample of COVID-19 patients and viral load association with disease severity and progression.
Methods: Blood samples from COVID-19 positive inpatients were collected sequentially, at admission, and during hospitalization. Depending on the clinical presentation, the patients were classified into mild, moderate, and severe categories. The blood samples were subjected to SARS-CoV-2 Real-time polymerase chain reaction (RT-PCR); positive samples were then tested for viral load using digital drop RT-PCR. Patients were followed up until they were discharged.
Results: Of 83 enrolled patients, 15 (18.1%) were mild, 45 (54%) were moderate, and 23 (27.7%) had severe symptoms. The blood PCR in 21 patients was positive (rate was 25.3); one was mild, four moderate, and 16 severe on the day of admission. The mean cycle threshold (CT) of RNAemia in these 21 patients was 24.7+3.1. The viral load was significantly higher in patients with severe symptoms than others. Serum ferritin, D-dimer, and plasma fibrinogen were found raised along with lymphopenia in all patients. Viremia was found persistently detectable (or even rising) in patients in the severe category and associated with a fatal outcome.
Conclusion: A prolonged elevation of D-dimer, serum ferritin, and plasma fibrinogen together with lymphopenia lead to a greater risk of mortality and morbidity. Quantitative estimation of virus in blood has a clinical significance which may help in prognostication and management of patients of COVID-19. J Microbiol Infect Dis 2021; 11(3):116-123.

Keywords

Viremia, Digital drop PCR, Serum ferritin, D-dimer, Lymphopenia, Plasma fibrinogen, Procalcitonin

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There are 21 citations in total.

Details

Primary Language	English
Subjects	Health Care Administration
Journal Section	Research Article
Authors	Jyotsna Agarwal This is me Vikramjeet Singh This is me Jaya Garg This is me Ritu Karoli2 This is me Swasti Tiwari This is me Shareh Naqvi This is me Anupam Das This is me Manodeep Sen This is me
Publication Date	September 15, 2021
Published in Issue	Year 2021 Volume: 11 Issue: 03

Cite

APA	Agarwal, J., Singh, V., Garg, J., Karoli2, R., et al. (2021). RNAemia and Clinical Outcome in COVID-19 Patients. Journal of Microbiology and Infectious Diseases, 11(03), 116-123. https://doi.org/10.5799/jmid.993839
AMA	Agarwal J, Singh V, Garg J, Karoli2 R, Tiwari S, Naqvi S, Das A, Sen M. RNAemia and Clinical Outcome in COVID-19 Patients. J Microbil Infect Dis. September 2021;11(03):116-123. doi:10.5799/jmid.993839
Chicago	Agarwal, Jyotsna, Vikramjeet Singh, Jaya Garg, Ritu Karoli2, Swasti Tiwari, Shareh Naqvi, Anupam Das, and Manodeep Sen. “RNAemia and Clinical Outcome in COVID-19 Patients”. Journal of Microbiology and Infectious Diseases 11, no. 03 (September 2021): 116-23. https://doi.org/10.5799/jmid.993839.
EndNote	Agarwal J, Singh V, Garg J, Karoli2 R, Tiwari S, Naqvi S, Das A, Sen M (September 1, 2021) RNAemia and Clinical Outcome in COVID-19 Patients. Journal of Microbiology and Infectious Diseases 11 03 116–123.
IEEE	J. Agarwal, “RNAemia and Clinical Outcome in COVID-19 Patients”, J Microbil Infect Dis, vol. 11, no. 03, pp. 116–123, 2021, doi: 10.5799/jmid.993839.
ISNAD	Agarwal, Jyotsna et al. “RNAemia and Clinical Outcome in COVID-19 Patients”. Journal of Microbiology and Infectious Diseases 11/03 (September 2021), 116-123. https://doi.org/10.5799/jmid.993839.
JAMA	Agarwal J, Singh V, Garg J, Karoli2 R, Tiwari S, Naqvi S, Das A, Sen M. RNAemia and Clinical Outcome in COVID-19 Patients. J Microbil Infect Dis. 2021;11:116–123.
MLA	Agarwal, Jyotsna et al. “RNAemia and Clinical Outcome in COVID-19 Patients”. Journal of Microbiology and Infectious Diseases, vol. 11, no. 03, 2021, pp. 116-23, doi:10.5799/jmid.993839.
Vancouver	Agarwal J, Singh V, Garg J, Karoli2 R, Tiwari S, Naqvi S, Das A, Sen M. RNAemia and Clinical Outcome in COVID-19 Patients. J Microbil Infect Dis. 2021;11(03):116-23.

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