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Role of Cycle Threshold of RT-PCR in the Prediction of COVID-19 Cases

Year 2021, Volume 11, Issue 03, 132 - 139, 15.09.2021
https://doi.org/10.5799/jmid.993878

Abstract

Objectives: It is currently unclear whether SARS-CoV-2 Cycle threshold (Ct) values could be leveraged to guide patients’ clinical manifestations and management decisions. The present study was undertaken to observe whether RT-PCR Ct values differ in COVID-19 symptomatic and asymptomatic individuals. This study also describes the clinical manifestations and epidemiological characteristics of both groups of patients. Methods: Nasopharyngeal or oropharyngeal swabs of 35,391 clinically suspected COVID-19 patients were collected in VTM and tested in our dedicated COVID-19 diagnostic laboratory for SARS-CoV-2 RT-PCR test. Cycle threshold at day 1 of positive detection was correlated with the presence or absence of the patient’s symptoms. The Ct values were studied sequentially in the collected samples to understand the variation of Ct values with the patient’s first day of diagnosis up to the recovery day. Results: Of 35,391 samples received for RT-PCR, data from 589 COVID-19 diagnosed patients (positivity 1.66%) was analyzed further. Among 589 patients, a total of 178 patients were symptomatic, while 411 were asymptomatic. Ct values obtained in symptomatic individuals (19.26±4.34) were significantly lower than in the asymptomatic (25.96±4.64) individuals (p<0.01). For 38 SARS-CoV-2 positive patients, data from sequential samples submitted to the laboratory was also analyzed. After adjusting for age, gender, co-morbidities, increased cycle threshold was associated with decreased odds of symptoms and in-hospital admission (0.91, CI 0.89– 0.94, p<0.001). Conclusion: Clinical symptoms and laboratory results of RT-PCR (Ct Value) of symptomatic patients with COVID-19 are significantly lower than those without symptoms. Analyzing data from 38 SARS-CoV-2 positive patients’ sequential samples again demonstrated that symptoms correlate with lower Ct values; however, virus clearance took almost the same time in the two groups of individuals. J Microbiol Infect Dis 2021; 11(3):132-139.

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Details

Primary Language English
Subjects Health Care Sciences and Services
Journal Section Research Article
Authors

Vikramjeet SINGH This is me
Dr. Ram Manohar Lohia Institute Of Medical Sciences, Lucknow, Uttar Pradesh, India
India


Jyotsna AGARWAL This is me
Dr. Ram Manohar Lohia Institute Of Medical Sciences, Lucknow, Uttar Pradesh, India
India


Jaya GARG This is me
Dr. Ram Manohar Lohia Institute Of Medical Sciences, Lucknow, Uttar Pradesh, India
India


Mohammad SAQUİB This is me
Dr. Ram Manohar Lohia Institute Of Medical Sciences, Lucknow, Uttar Pradesh, India
India


Anupam DAS This is me
Dr. Ram Manohar Lohia Institute Of Medical Sciences, Lucknow, Uttar Pradesh, India
India


Manodeep SEN This is me
Dr. Ram Manohar Lohia Institute Of Medical Sciences, Lucknow, Uttar Pradesh, India
India

Publication Date September 15, 2021
Published in Issue Year 2021, Volume 11, Issue 03

Cite

Bibtex @research article { jmid993878, journal = {Journal of Microbiology and Infectious Diseases}, issn = {2146-3158}, eissn = {2146-9369}, address = {}, publisher = {Sağlık Araştırmaları Derneği}, year = {2021}, volume = {11}, pages = {132 - 139}, doi = {10.5799/jmid.993878}, title = {Role of Cycle Threshold of RT-PCR in the Prediction of COVID-19 Cases}, key = {cite}, author = {Sıngh, Vikramjeet and Agarwal, Jyotsna and Garg, Jaya and Saquib, Mohammad and Das, Anupam and Sen, Manodeep} }
APA Sıngh, V. , Agarwal, J. , Garg, J. , Saquib, M. , Das, A. & Sen, M. (2021). Role of Cycle Threshold of RT-PCR in the Prediction of COVID-19 Cases . Journal of Microbiology and Infectious Diseases , 11 (03) , 132-139 . DOI: 10.5799/jmid.993878
MLA Sıngh, V. , Agarwal, J. , Garg, J. , Saquib, M. , Das, A. , Sen, M. "Role of Cycle Threshold of RT-PCR in the Prediction of COVID-19 Cases" . Journal of Microbiology and Infectious Diseases 11 (2021 ): 132-139 <https://dergipark.org.tr/en/pub/jmid/issue/64866/993878>
Chicago Sıngh, V. , Agarwal, J. , Garg, J. , Saquib, M. , Das, A. , Sen, M. "Role of Cycle Threshold of RT-PCR in the Prediction of COVID-19 Cases". Journal of Microbiology and Infectious Diseases 11 (2021 ): 132-139
RIS TY - JOUR T1 - Role of Cycle Threshold of RT-PCR in the Prediction of COVID-19 Cases AU - Vikramjeet Sıngh , Jyotsna Agarwal , Jaya Garg , Mohammad Saquib , Anupam Das , Manodeep Sen Y1 - 2021 PY - 2021 N1 - doi: 10.5799/jmid.993878 DO - 10.5799/jmid.993878 T2 - Journal of Microbiology and Infectious Diseases JF - Journal JO - JOR SP - 132 EP - 139 VL - 11 IS - 03 SN - 2146-3158-2146-9369 M3 - doi: 10.5799/jmid.993878 UR - https://doi.org/10.5799/jmid.993878 Y2 - 2021 ER -
EndNote %0 Journal of Microbiology and Infectious Diseases Role of Cycle Threshold of RT-PCR in the Prediction of COVID-19 Cases %A Vikramjeet Sıngh , Jyotsna Agarwal , Jaya Garg , Mohammad Saquib , Anupam Das , Manodeep Sen %T Role of Cycle Threshold of RT-PCR in the Prediction of COVID-19 Cases %D 2021 %J Journal of Microbiology and Infectious Diseases %P 2146-3158-2146-9369 %V 11 %N 03 %R doi: 10.5799/jmid.993878 %U 10.5799/jmid.993878
ISNAD Sıngh, Vikramjeet , Agarwal, Jyotsna , Garg, Jaya , Saquib, Mohammad , Das, Anupam , Sen, Manodeep . "Role of Cycle Threshold of RT-PCR in the Prediction of COVID-19 Cases". Journal of Microbiology and Infectious Diseases 11 / 03 (September 2021): 132-139 . https://doi.org/10.5799/jmid.993878
AMA Sıngh V. , Agarwal J. , Garg J. , Saquib M. , Das A. , Sen M. Role of Cycle Threshold of RT-PCR in the Prediction of COVID-19 Cases. J Microbil Infect Dis. 2021; 11(03): 132-139.
Vancouver Sıngh V. , Agarwal J. , Garg J. , Saquib M. , Das A. , Sen M. Role of Cycle Threshold of RT-PCR in the Prediction of COVID-19 Cases. Journal of Microbiology and Infectious Diseases. 2021; 11(03): 132-139.
IEEE V. Sıngh , J. Agarwal , J. Garg , M. Saquib , A. Das and M. Sen , "Role of Cycle Threshold of RT-PCR in the Prediction of COVID-19 Cases", Journal of Microbiology and Infectious Diseases, vol. 11, no. 03, pp. 132-139, Sep. 2021, doi:10.5799/jmid.993878