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First time isolation of Pseudomonas argentinensis from human skin infection

Year 2014, Volume: 4 Issue: 02, 78 - 83, 01.06.2014
https://doi.org/10.5799/ahinjs.02.2014.02.0133

Abstract

This reported the first isolation of Pseudomonas argentinensis from a human infection, since the discovery of P. argentinensis in 2005. A Palestinian 63-year male practicing gardening on sunny days with exposed arms and legs had experienced recurrent skin infections of forearms and legs. The skin infection progressed from small itchy red rash into fluid-filled vesicles within three days. One type of bacteria was recovered from culture samples of these vesicles. The infection was treated successfully with 2% fucidine. Using the Universal Method, the isolate (labeled as QUBC88) was identified as Pseudomonas argentinensis. The patient\'s son had suffered a similar skin infection while gardening in a different continent. The inability of the isolate QUBC88 to grow at 37°C may explain its inability to cause systemic infection. Future skin infections of the patients will be followed up to determine if the infectious agent is the same, related, or not related to P. argentinensis.

References

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  • Barghouthi SA. A Universal Method for the Identification of Bacteria Based on General PCR Primers. Ind J Microbio 2011;51:430-444.
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  • El-Hady MA, Samy AA. Molecular Typing of Pseudomonas Species Isolated from Some Cultured Fishes in Egypt. Glob- al Vet 2011;7:576-580.
  • Levy PY, Fenollar F, Stein A, et al. Propionibacterium acnes postoperative shoulder arthritis: an emerging clinical entity. Clin Infect Dis 2008;46:1884-1886.
  • Peix A, Berge O, Rivas R, et al. Pseudomonas argentinensis sp. nov., a novel yellow pigment-producing bacterial species, isolated from rhizospheric soil in Cordoba, Argentina. Int J Syst Evol Microbiol 2005;55:1107-1112.
  • Hariharan H, Matthew V, Fountain J, et al. Aerobic bacteria from mucous membranes, ear canals, and skin wounds of feral cats in Grenada, and the antimicrobial drug suscepti- bility of major isolates. Comp Immunol Microbiol Infect Dis 2011;34:129-134.
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  • Sommkr S, Bojar R, Cunliffe WJ, et al. Investigation of the mechanism of action of 2% fusidic acid lotion in the treatment of acne vulgaris. Clin Exper Derm 1997,22:211-215.
  • Nayak N, Verma L, Satpathy G, Venkatesh P. Propionibac- terium acnes endophthalmitis. J Clin Diag Res 2007;3:155- 158.
  • Barghouthi SA, Hammad G, Kurdi M. Acinetobacter iwoffii induced cellulitis with allergy-like symptoms. Internet J Mi- crobiol 2012;10:12-17.

First time isolation of Pseudomonas argentinensis from human skin infection

Year 2014, Volume: 4 Issue: 02, 78 - 83, 01.06.2014
https://doi.org/10.5799/ahinjs.02.2014.02.0133

Abstract

Bu 2005 yılında Pseudomonas argentinensis’in tanımlanmasından sonra insan kaynaklı tekrarlayan cilt enfeksiyonundan izole edilen ilk P. argentinensis olgusu bildirimidir. Filistinli 63 yaşındaki erkek bir hastada güneşli günlerde bahçede çalışırken ön kollarda ve bacaklarda tekrarlayan cilt enfeksiyonları gelişti. Üç gün içinde, cilt enfeksiyonu küçük kaşıntılı kırmızı raş lezyonlardan içi sıvı dolu veziküllere dönüştü. Bu veziküllerden alınan kültürde bir tip bakteri izole edildi. Bu lezyonlar %2 fusidin ile düzeldi. Üniversal Metot kullanılarak izolat (QUBC88 olarak etiketlendi) Pseudomonas argentinensis olarak belirlendi. Hastanın başka bir kıtada yaşayan oğlunda da bahçede çalışırken benzer cilt lezyonları gelişti. İzolat QUBC88’in 37°C’de üreyememesi sistemik enfeksiyon oluşturmama nedenini açıklayabilir. Hastalarda daha sonra gelişecek cilt enfeksiyonları izole edilecek mikroorganizmanın yanı bakteri ya da P. argentinensis ile bağlantılı olup olmadığını belirlemek üzere takip edilecektir

References

  • API 20NE: Identification system for non-fastidious, non-enteric Gram-negative rods. http://www.netropica.org/ bacteriologia/ Api20NE%281%29.pdf. Accessed 6 November 2013.
  • Rolfe B, Holloway BW. Alterations in host specificity of bacte- rial deoxyribonucleic acid after an increase in growth temper- ature of Pseudomonas aeruginosa. J Bacteriol 1966;92:43- 48.
  • Barghouthi SA. A Universal Method for the Identification of Bacteria Based on General PCR Primers. Ind J Microbio 2011;51:430-444.
  • BLAST. http://www.ncbi.nlm.nih.gov/blast/Blast. cgi?PROGRAM=blastn,BLAST_PROGRAMS=megaBlast PAGE_TYPE= BlastSearch, SHOW_DEFAULTS=on, LINK_ LOC=blasthome . Accessed 6 November 2013.
  • Clustalw. http://www.genome.jp/tools/clustalw/. Accessed 6 Nov, 2013.
  • Hudzicki J. http://www.microbelibrary.org/component/re- source/laboratory-test/3189-kirby-bauer-disk-diffusion-sus- ceptibility-test-protocol. Accessed 6 Nov 2013.
  • European Committee on Antimicrobial Susceptibility Testing (EUCAST). Breakpoint tables for interpretation of MICs and zone diameters Version 3.1. http://www.eucast.org/fileadmin/ src/media/PDFs/EUCAST_files/Breakpoint_tables/Break- point _table_v_3.1.pdf. Accessed 6 November 2013.
  • Tripathi P, Banerjee G, Saxena S, et al. Antibiotic resistance pattern of Pseudomonas aeruginosa isolated from pa- tients of lower respiratory tract infection. Afr J Microb Res 2011;5:2955-2959.
  • El-Hady MA, Samy AA. Molecular Typing of Pseudomonas Species Isolated from Some Cultured Fishes in Egypt. Glob- al Vet 2011;7:576-580.
  • Levy PY, Fenollar F, Stein A, et al. Propionibacterium acnes postoperative shoulder arthritis: an emerging clinical entity. Clin Infect Dis 2008;46:1884-1886.
  • Peix A, Berge O, Rivas R, et al. Pseudomonas argentinensis sp. nov., a novel yellow pigment-producing bacterial species, isolated from rhizospheric soil in Cordoba, Argentina. Int J Syst Evol Microbiol 2005;55:1107-1112.
  • Hariharan H, Matthew V, Fountain J, et al. Aerobic bacteria from mucous membranes, ear canals, and skin wounds of feral cats in Grenada, and the antimicrobial drug suscepti- bility of major isolates. Comp Immunol Microbiol Infect Dis 2011;34:129-134.
  • Hot tub rash. http://www.skinsight.com/adult/pseudomonas- FolliculitisHotTubFolliculitis.htm. Accessed 6 November 2013.
  • Sommkr S, Bojar R, Cunliffe WJ, et al. Investigation of the mechanism of action of 2% fusidic acid lotion in the treatment of acne vulgaris. Clin Exper Derm 1997,22:211-215.
  • Nayak N, Verma L, Satpathy G, Venkatesh P. Propionibac- terium acnes endophthalmitis. J Clin Diag Res 2007;3:155- 158.
  • Barghouthi SA, Hammad G, Kurdi M. Acinetobacter iwoffii induced cellulitis with allergy-like symptoms. Internet J Mi- crobiol 2012;10:12-17.
There are 16 citations in total.

Details

Primary Language English
Journal Section Case Report
Authors

Sameer A. Barghouthi This is me

KHALID Younıs This is me

Publication Date June 1, 2014
Published in Issue Year 2014 Volume: 4 Issue: 02

Cite

APA Barghouthi, S. A., & Younıs, K. (2014). First time isolation of Pseudomonas argentinensis from human skin infection. Journal of Microbiology and Infectious Diseases, 4(02), 78-83. https://doi.org/10.5799/ahinjs.02.2014.02.0133
AMA Barghouthi SA, Younıs K. First time isolation of Pseudomonas argentinensis from human skin infection. J Microbil Infect Dis. June 2014;4(02):78-83. doi:10.5799/ahinjs.02.2014.02.0133
Chicago Barghouthi, Sameer A., and KHALID Younıs. “First Time Isolation of Pseudomonas Argentinensis from Human Skin Infection”. Journal of Microbiology and Infectious Diseases 4, no. 02 (June 2014): 78-83. https://doi.org/10.5799/ahinjs.02.2014.02.0133.
EndNote Barghouthi SA, Younıs K (June 1, 2014) First time isolation of Pseudomonas argentinensis from human skin infection. Journal of Microbiology and Infectious Diseases 4 02 78–83.
IEEE S. A. Barghouthi and K. Younıs, “First time isolation of Pseudomonas argentinensis from human skin infection”, J Microbil Infect Dis, vol. 4, no. 02, pp. 78–83, 2014, doi: 10.5799/ahinjs.02.2014.02.0133.
ISNAD Barghouthi, Sameer A. - Younıs, KHALID. “First Time Isolation of Pseudomonas Argentinensis from Human Skin Infection”. Journal of Microbiology and Infectious Diseases 4/02 (June 2014), 78-83. https://doi.org/10.5799/ahinjs.02.2014.02.0133.
JAMA Barghouthi SA, Younıs K. First time isolation of Pseudomonas argentinensis from human skin infection. J Microbil Infect Dis. 2014;4:78–83.
MLA Barghouthi, Sameer A. and KHALID Younıs. “First Time Isolation of Pseudomonas Argentinensis from Human Skin Infection”. Journal of Microbiology and Infectious Diseases, vol. 4, no. 02, 2014, pp. 78-83, doi:10.5799/ahinjs.02.2014.02.0133.
Vancouver Barghouthi SA, Younıs K. First time isolation of Pseudomonas argentinensis from human skin infection. J Microbil Infect Dis. 2014;4(02):78-83.