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In-vitro susceptibility of multiple drug resistant Pseudomonas aeruginosa to organic acids

Year 2013, Volume: 3 Issue: 02, 67 - 70, 01.06.2013
https://doi.org/10.5799/ahinjs.02.2013.02.0083

Abstract

Objective: Pseudomonas aeruginosa is a classic opportunistic pathogen with innate resistance to many antibiotics and disinfectants. Resistance to antimicrobial agents makes it the most noxious organism to eliminate from infection site. In view of its antimicrobial resistance, an attempt was made to study its susceptibility to various organic acids. Methods: Seven clinical isolates of P.aeruginosa resistant to multiple antibiotics were subjected to in vitro susceptibility to various organic acids by broth dilution method to find out susceptibility to various organic acids. Results: The isolates of P.aeruginosa resistant to 14 antimicrobials were found susceptible to one percent oxalic acid and trichloroacetic acid, two percent lactic acid and citric acid, and three percent acetic acid. It is interesting to note that strains resistant to multiple antibiotics were also found susceptible to organic acids. Oxalic acid and trichloroacetic acid were found highly effective. Conclusions: Clinical use of oxalic acid, trichloroacetic acid and lactic acid as topical agents to treat superficial pseu­domonal infections caused by difficult strains of P.aeruginosa may be recommended after confirmation of their toxicity and in vivo efficacy in animal models.

References

  • Krasilnikov AP, Adarchenko AA, Bulai PI, Sobeshchuk OP. A comparative analysis of the antibacterial activity of anti-sep- tics and antibiotics on samples of Pseudomonas aeruginosa. Zh Mikrobiol Epidemiol Immunobiol 1991;8:30-33.
  • Ananthnarayan R, Jayaram Panikar CK. Textbook of Micro- biology. 8th edn. Madras: Universities Press, 2009:315-319.
  • Palleroni NJ. Pseudomonas. In: Borriello SP, Murray PR and Funke G. editors. Topley and Wilson’s Microbiology and Mi- crobial Infections. 10th ed, Vol II. Bacteriology. London: Ed- ward Arnold; 2005:1591-1606.
  • Pitt TL. Pseudomonas. In Parakar MT, Collier EH, eds: Topley and Wilsons Principles of Bacteriology, Virology and Immu- nity. 8th edn, Vol II. London; Edward Arnold, 1990:256-273.
  • Sloss JM, Cumberland N, Milner SM. Acetic acid used for the elimination of Pseudomonas aeruginosa from burn and soft tissue wounds. J Army Med Crops 1993;139:49-51.
  • Al-Ibran E, Khan M. Efficacy of topical application of 1% acetic acid in eradicating pseudomonal infections in burn wounds. J Dow Uni Health Sci 2010;4:90-93.
  • Juma IM, Yass HS, Al-Jaberi FS. A comparison between the effect of acetic acid and salicyclic acid in different concentra- tions on Pseudomonas aeruginosa isolated from burn wound infections. http://www.iasj.net/iasj?func=fulltext&aId=41515. Retrieved on 21 Aug 2012.
  • Nagoba B, Wadher B, Kulkarni P, Kolhe S. Acetic acid treat- ment of pseudomonal wound infections. Eur J Gen Med 2008;5:104-106.
  • Nagoba BS, Gandhi RC, Wadher BJ, Gandhi SP, Deshmukh SR. Citric acid treatment of severe electric burns complicat- ed by multiple antibiotic resistant Pseudomonas aeruginosa. Burns 1998;24:481-483.
  • Nagoba BS, Wadher BJ, Deshmukh SR, et al. Treatment of superficial pseudomonal infections with citric acid – an effec- tive and economical approach. J Hosp Infect 1998; 40:155- 157.
  • Govan JRW. Pseudomonas. In: Collee JG, Fraser AG, Marmion BP and Simmons A, eds. Mackie and McCart- ney Practical Medical Microbiology, 14th edn. New Delhi: Churchill-Livingstone, 2006:413-424.
  • Bauer AW, Kirby WMM, Sherris JC, Turck M. Antibiotic sus- ceptibility testing by a standardized single disk method. Am J Clin Pathol 1966;45:493-496.
  • Sonnerwirth AC and Jarett L. Gradwohl’s Clinical Laboratory Methods and Diagnosis. 8th Edn, Vol II. London: The C. V. Mosby Company, 1980:1809-1822.

In-vitro susceptibility of multiple drug resistant Pseudomonas aeruginosa to organic acids

Year 2013, Volume: 3 Issue: 02, 67 - 70, 01.06.2013
https://doi.org/10.5799/ahinjs.02.2013.02.0083

Abstract

Amaç: Pseudomonas aeruginosa fırsatçı bir patojen olup birçok antibiyotiğe ve dezenfektana doğal dirençlidir. Antimikrobiyal direnci nedeniyle bu etkenleri elimine etmek zordur. Antimikrobiyal direnci göz önüne alınarak bakterilerin çeşitli organik asitlere duyarlılığının araştırılması amaçlanmıştır.Yöntemler: Toplam yedi dirençli P. aeruginosa izolatının çeşitli organik asitlere karşı etkinliği sıvı mikrodilüsyon tekniği ile araştırıldı.Bulgular: Farklı 14 antibiyotiğe dirençli P. aeruginosa izolatının %1 oranında oksalik asit ve trikloroasetik asite, %2 laktik asit ve sitrik aside %3 oranında asetik asite hassas olduğu gözlendi. İlginç olan nokta ise birçok antibiyotiğe dirençli olan bu kökenler organik asitlere hassastı. Oksalik asid ve trikloroasetik asit oldukça etkin bulundu.Sonuç: Bulgularımız toksisite ve invivo etkinlik testleri ile doğrulanmasının ardından oksalik asit, trikloro asetik asit ve laktik asit tedavisi güç P. aeruginosa enfeksiyonlarının topikal tedavisinde kullanılması önerilebilir

References

  • Krasilnikov AP, Adarchenko AA, Bulai PI, Sobeshchuk OP. A comparative analysis of the antibacterial activity of anti-sep- tics and antibiotics on samples of Pseudomonas aeruginosa. Zh Mikrobiol Epidemiol Immunobiol 1991;8:30-33.
  • Ananthnarayan R, Jayaram Panikar CK. Textbook of Micro- biology. 8th edn. Madras: Universities Press, 2009:315-319.
  • Palleroni NJ. Pseudomonas. In: Borriello SP, Murray PR and Funke G. editors. Topley and Wilson’s Microbiology and Mi- crobial Infections. 10th ed, Vol II. Bacteriology. London: Ed- ward Arnold; 2005:1591-1606.
  • Pitt TL. Pseudomonas. In Parakar MT, Collier EH, eds: Topley and Wilsons Principles of Bacteriology, Virology and Immu- nity. 8th edn, Vol II. London; Edward Arnold, 1990:256-273.
  • Sloss JM, Cumberland N, Milner SM. Acetic acid used for the elimination of Pseudomonas aeruginosa from burn and soft tissue wounds. J Army Med Crops 1993;139:49-51.
  • Al-Ibran E, Khan M. Efficacy of topical application of 1% acetic acid in eradicating pseudomonal infections in burn wounds. J Dow Uni Health Sci 2010;4:90-93.
  • Juma IM, Yass HS, Al-Jaberi FS. A comparison between the effect of acetic acid and salicyclic acid in different concentra- tions on Pseudomonas aeruginosa isolated from burn wound infections. http://www.iasj.net/iasj?func=fulltext&aId=41515. Retrieved on 21 Aug 2012.
  • Nagoba B, Wadher B, Kulkarni P, Kolhe S. Acetic acid treat- ment of pseudomonal wound infections. Eur J Gen Med 2008;5:104-106.
  • Nagoba BS, Gandhi RC, Wadher BJ, Gandhi SP, Deshmukh SR. Citric acid treatment of severe electric burns complicat- ed by multiple antibiotic resistant Pseudomonas aeruginosa. Burns 1998;24:481-483.
  • Nagoba BS, Wadher BJ, Deshmukh SR, et al. Treatment of superficial pseudomonal infections with citric acid – an effec- tive and economical approach. J Hosp Infect 1998; 40:155- 157.
  • Govan JRW. Pseudomonas. In: Collee JG, Fraser AG, Marmion BP and Simmons A, eds. Mackie and McCart- ney Practical Medical Microbiology, 14th edn. New Delhi: Churchill-Livingstone, 2006:413-424.
  • Bauer AW, Kirby WMM, Sherris JC, Turck M. Antibiotic sus- ceptibility testing by a standardized single disk method. Am J Clin Pathol 1966;45:493-496.
  • Sonnerwirth AC and Jarett L. Gradwohl’s Clinical Laboratory Methods and Diagnosis. 8th Edn, Vol II. London: The C. V. Mosby Company, 1980:1809-1822.
There are 13 citations in total.

Details

Primary Language English
Journal Section ART
Authors

Basavraj S Nagoba This is me

Bharat Wadher This is me

Sohan Selkar This is me

Publication Date June 1, 2013
Published in Issue Year 2013 Volume: 3 Issue: 02

Cite

APA Nagoba, B. S., Wadher, B., & Selkar, S. (2013). In-vitro susceptibility of multiple drug resistant Pseudomonas aeruginosa to organic acids. Journal of Microbiology and Infectious Diseases, 3(02), 67-70. https://doi.org/10.5799/ahinjs.02.2013.02.0083
AMA Nagoba BS, Wadher B, Selkar S. In-vitro susceptibility of multiple drug resistant Pseudomonas aeruginosa to organic acids. J Microbil Infect Dis. June 2013;3(02):67-70. doi:10.5799/ahinjs.02.2013.02.0083
Chicago Nagoba, Basavraj S, Bharat Wadher, and Sohan Selkar. “In-Vitro Susceptibility of Multiple Drug Resistant Pseudomonas Aeruginosa to Organic Acids”. Journal of Microbiology and Infectious Diseases 3, no. 02 (June 2013): 67-70. https://doi.org/10.5799/ahinjs.02.2013.02.0083.
EndNote Nagoba BS, Wadher B, Selkar S (June 1, 2013) In-vitro susceptibility of multiple drug resistant Pseudomonas aeruginosa to organic acids. Journal of Microbiology and Infectious Diseases 3 02 67–70.
IEEE B. S. Nagoba, B. Wadher, and S. Selkar, “In-vitro susceptibility of multiple drug resistant Pseudomonas aeruginosa to organic acids”, J Microbil Infect Dis, vol. 3, no. 02, pp. 67–70, 2013, doi: 10.5799/ahinjs.02.2013.02.0083.
ISNAD Nagoba, Basavraj S et al. “In-Vitro Susceptibility of Multiple Drug Resistant Pseudomonas Aeruginosa to Organic Acids”. Journal of Microbiology and Infectious Diseases 3/02 (June 2013), 67-70. https://doi.org/10.5799/ahinjs.02.2013.02.0083.
JAMA Nagoba BS, Wadher B, Selkar S. In-vitro susceptibility of multiple drug resistant Pseudomonas aeruginosa to organic acids. J Microbil Infect Dis. 2013;3:67–70.
MLA Nagoba, Basavraj S et al. “In-Vitro Susceptibility of Multiple Drug Resistant Pseudomonas Aeruginosa to Organic Acids”. Journal of Microbiology and Infectious Diseases, vol. 3, no. 02, 2013, pp. 67-70, doi:10.5799/ahinjs.02.2013.02.0083.
Vancouver Nagoba BS, Wadher B, Selkar S. In-vitro susceptibility of multiple drug resistant Pseudomonas aeruginosa to organic acids. J Microbil Infect Dis. 2013;3(02):67-70.