The effects of four different drugs administered through catheters on slime production in coagulase negative Staphylococci

Year 2012, Volume: 2 Issue: 04, 150 - 154, 01.12.2012

Julide Sedef Göçmen Ünase Büyükkoçak Alpay Azap Yasemin Pekuz Osman Çağlayan

https://doi.org/10.5799/jmid.123128

Abstract

Keywords

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The effects of four different drugs administered through catheters on slime production in coagulase negative Staphylococci

Abstract

Objective: Higher rate of slime production has been found in pathogen bacteria strains. Accordingly, the factors that contribute to higher slime production rate increase the infection risk, while the factors that reduce the slime production rate will reduce the infection risk. The effect of some drugs that are administered through catheters in intensive care units on slime production with coagulase negative Staphylococci was investigated. Methods: In this study, the effect of four different preparations containing Glyceryl trinitrate (Perlinganit®), Dexmedetomidine (Precedex®), Esmolol (Brevibloc®), and Propofol (Propofol®) on slime production of 24 Staphylococcus epidermidis strains isolated from blood cultures of patients, and reference strain were investigated. Slime production was determined using ‘the quantitative microdilution plaque test\' described by Christensen. Results: Under controlled medium, eight strains formed slimes, and in the media containing esmolol, glyceryl trinitrate, dexmedetomidine, and propofol slimes were positive for five, 21, 15, and 18 strains, respectively. The rate of slime production in glyceryl trinitrate, dexmedetomidine, and propofol containing media were higher than that of the controls. Conclusions: In the light of the results of this study, it is concluded that the drugs and/or additives increase the rate of slime production. The effects of the preparations administered through catheters on slime production should be investigated, and these effects should be kept in mind during their use.

Keywords

catheter, slim production, staphylococci

References

• O’Grady NP, Alexander M, Dellinger EP, et al. Guidelines for the prevention of intravascular catheter-related infections. Infect Control Hosp Epidemiol 2002; 23:759-769.
• Beekmann SE, Henderson DK. Bacteremia due to percutane- ous intravascular devices. In: Mandell GL, Bennett JE, Dolin R, eds. Principles and Practice of Infectious Diseases, 6th edn. New York: Elsevier, Churchill, Livingstone, 2005:3347- 3361.
• Capdevila JA. Cathater-related infections: An update on diag- nosis, treatment and prevention. Int J Infect Dis 1998; 2:230- 236.
• Christensen GD, Simpson WA, Bisno AL, Beachey EH. Adher- ence of slime producing strains of Staphylococcus epidermi- dis to smooth surfaces. Infect Immun 1982;37:318-326.
• Christensen GD, Simpson WA, Younger JJ, et al. Adherence of coagulase-negative staphylococci to plastic tissue culture- plates: a quantitative model for the adherence of staphylo- cocci to medical devices. J Clin Microbiol 1985; 22:996-1006.
• Götz F, Peters G. Colonization of medical devices by coag- ulase-negative staphylococci. In: Waldvogel FA, Bisno AL, eds. Infections associated with indwelling medical devices, 3rd edn. Washington, DC: American Society for Microbiology, 2000:55-82.
• Hebert GA, Cooksey RC, Clark NC, Hill BC, Jarvis WR, Thorn- sberry C. Biotyping coagulase-negative staphylococci. J Clin Microbiol 1988;26: 1950-1956.
• Davenport DS, Massanari RM, Pfaller MA, Bale MJ, Streed SA, Hierholzer WJ Jr. Usefulness of a test for slime pro- duction as a marker for clinically significant infections with coagulase-negative staphylococci. J Infect Dis 1986;153: 332-339.
• Christensen GD, Barker LP, Mowhinney TP, Baddour LM, Simpson WA. Identification of an antigenic marker of slime production for Staphylococcus epidermidis. Infect Immun 1990; 58:2906-2911.
• Gedik A, Ersay A, Atmaca S, Sener A. Oxybutynin effects on Staphylococcus epidermidis biofilm production. South Med J 2008; 101:236-239.
• Roveta S, Marchese A, Schito GC. Activity of daptomycin on biofilms produced on a plastic support by Staphylococcus spp. Int J Antimicrob Agents 2008; 31:321-328.
• Demirag MK, Esen S, Zivalioglu M, Leblebicioglu H, Keceligil HT. The effect of aspirin on adherence of slime-producing, coagulase-negative staphylococci to vascular grafts. Ann Vasc Surg 2007; 21:464-467.
• Stepanović S, Vuković D, Hola V, et al. Quantification of bio- film in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm pro- duction by staphylococci. APMIS 2007; 115:891-899.
• Dorman NJ. Sepsis. In: Betts RF, Chapman SW, Penn RL, eds, Reese and Betts’ a practical approach to infectious dis- eases, 5th ed. Philadelphia: Lippincott Williams & Wilkins, 2003:19-67.
• Raad I, Hana H. Intravascular catheter-related infec- tions new horizons and recent advances. Arch Intern Med 2002;162:871-878.
• Klemm P, Vejborg RM, Hancock V. Prevention of bacterial adhesion. Appl Microbiol Biotechnol 2010; 88:451-459.
• Raad I, Chatzinikolaou I, Chaiban G, et al. In vitro and ex- vivo activities of minocycline and EDTA against microorgan- isms embedded in biofilm on catheter surfaces. Antimicrob Agents Chemother 2003; 47:3580-3585.