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Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs

Year 2011, Volume: 1 Issue: 01, 5 - 9, 01.03.2011
https://doi.org/10.5799/ahinjs.02.2011.01.0002

Abstract

Objectives: The aim of the present study was to evaluate the efficacy (recovery rate, time to detection and Drug Susceptibility Tests –DST- of Mycobacteria-only B460) of new colorimetric medium, Dio-TK and to compare it with routinely used conventional media, Lowenstein Jensen (LJ) and Bactec 460 TB culture system. Materials and methods: Totally 901 clinic specimens were investigated for assignment of tuberculosis by Ehrlich-Ziehl-Nielsen smear strain method, Lowenstein-Jensen, BACTEC 460TB and Dio-TK medium culture systems. Results: Nineteen of 901 clinic specimens (2.1%) were positive by any of these methods. 17 (89.5%) of these specimens positive found by smear strain method, 17 (89.5%) by Lowenstein-Jensen, 19 (100%) by BACTEC 460TB and 14 (73.7%) by Dio-TK medium. NAP and Niacin identification tests were applied to Mycobacterium strains. 12 (63.1%) of 19 isolates were identified as M.tuberculosis complex and 7 (36.9%) were identified as Mycobacterium other than tuberculosis (MOTT) bacilli. 10 (83.3%) of 12 M.tuberculosis complex strains were not resistant to any major drug. But one of 2 isolate was resistant to streptomycin and the other one isolate was resistant to both streptomycin and isoniazid. Conclusion: Our data suggest that some advantages (such as an early detection and differentiation mycobacterium growth from contamination) of the Dio-TK CS over other mycobacterial culture systems make it a practical and rapid system for daily use, and a suitable alternative to other currently available solid media, such as LJ, for detection time of mycobacteria and DST.

References

  • Metchock, B. G., F. S. Nolte, and R. J. Wallace III. Mycobacte- rium. In P. R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (ed.), Manual of Clinical Microbiology, p. 399-437, 7th ed. ASM Press, Washington, D.C, 1999.
  • Baylan O, Kisa O, Albay A, Doganci L. Evaluation of a new automated, rapid, colorimetric culture system using solid me- dium for laboratory diagnosis of tuberculosis and determined of anti-tuberculosis drug susceptibility. Int J Tuberc Lung Dis 2004; 8: 772-777.
  • Badak, F.Z., Kiosk, D.L., Setterquist, S., Hartley, C., O’Connell, M.A. and Hopfer, R.L., Comparison of Mycobacteria Growth Indicator Tube with BACTEC 460 for detection and recov- ery of mycobacteria from clinical specimens. J Clin Microbiol 1996; 34: 2236–2239.
  • Rohner, P., Ninet, B., Metral, C., Emler, S. and Auckenthaler, R. Evaluation of the MB/BacT system in comparison to the BACTEC 460 system and solid media for isolation of myco- bacteria from clinical specimens. J Clin Microbiol 1997; 35: 3127–3131.
  • Centers for Disease Control and Prevention (CDC) Essential components of a tuberculosis prevention and control pro- gram. Morbid Mortal Weekly Rep 1995; 44: 11-16.
  • Rüsch-Gerdes S, Ebraihmzadeh A, Elliott LB, Hanna BA, Mor- gan MA, Novak S. Multicenter evaluation of the BACTEC MGIT 960 system compared to the BACTEC 460 TB and solid media for recovery of mycobacteria. In: Abstracts of the 19th Congress of the European Society of Mycobacteriology. Rev Port Pneumol 1998; 4: 346.
  • Scarparo C, Ricordi P, Ruggiero G, Piccoli P. Evaluation of the fully automated BACTEC MGIT 960 system for testing sus- ceptibility of Mycobacterium tuberculosis to pyrazinamide, streptomycin, isoniazid, rifampin, and ethambutol and com- parison with the radiometric BACTEC 460TB method.J Clin Microbiol. 2004; 42:1109-1114.
  • Kocagoz T, Alp A, Albay A. A new rapid non-radioactive me- dium for culturing mycobacteria, that also enables visual dif- ferentiation of mycobacterial growth from contamination (Ab- stract) . Los Angeles, CA: American Society of Microbiology General Meeting. May, 21-25, 2000.
  • Ercis S, Alp A, Hascelik G, Kocagoz T. Comparison of Dio- TK rapid mycobacterium culture system with BACTEC 460 TB system and LJ medium in diagnosis of tuberculosis and detection of susceptibility of antituberculosis drugs (Abstract) Abant, Bolu, Turkey; 4th National Mycobacterium Sympo- sium, 2002: 180-181.
  • Bicmen C, Coskun M, Senol G, Florat N, Kocagoz T. Com- parison of Dio-TK and LJ media for primary culture of my- cobacteria: a preliminary study for a new medium (Abstract) Glasgow, UK: 13th ECCMID, May, 2003.
  • Baylan O, Kisa O, Albay A, L. Doganci. Evaluation of a new automated, rapid, colorimetric culture system using solid me- dium for laboratory diagnosis of tuberculosis and determina- tion of anti-tuberculosis drug susceptibility. Int J Tuberc Lung Dis 2004; 8:772–777.
  • Solis LA, Shin SS, Han LL, Llanos F, Stowell M, Sloutsky A. Validation of a rapid method for detection of M.tuberculosis resistance to isoniazid and rifampin in Lima, Peru.Int J Tu- berc Lung Dis 2005; 9: 760-764.
  • Negi SS, Khan SF, Gupta S, Pasha ST, Khare S, Lal S. Com- parison of the conventional diag-nostic modalities, bactec culture and polymerase chain reaction test for diagnosis of tuberculosis. Indian J Med Microbiol 2005;23:29-33.
  • Ani A, Okpe S, Akambi M, Ejelionu E, Yakubu B, Owolodun O, Ekeh P, Oche A, Tyen D, Idoko J. Comparison of a DNA based PCR method with conventional methods for the detec- tion of M.tuberculosis in Jos, Nigeria. J Infect Dev Countries 2009; 3: 470-475.

Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs

Year 2011, Volume: 1 Issue: 01, 5 - 9, 01.03.2011
https://doi.org/10.5799/ahinjs.02.2011.01.0002

Abstract

References

  • Metchock, B. G., F. S. Nolte, and R. J. Wallace III. Mycobacte- rium. In P. R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (ed.), Manual of Clinical Microbiology, p. 399-437, 7th ed. ASM Press, Washington, D.C, 1999.
  • Baylan O, Kisa O, Albay A, Doganci L. Evaluation of a new automated, rapid, colorimetric culture system using solid me- dium for laboratory diagnosis of tuberculosis and determined of anti-tuberculosis drug susceptibility. Int J Tuberc Lung Dis 2004; 8: 772-777.
  • Badak, F.Z., Kiosk, D.L., Setterquist, S., Hartley, C., O’Connell, M.A. and Hopfer, R.L., Comparison of Mycobacteria Growth Indicator Tube with BACTEC 460 for detection and recov- ery of mycobacteria from clinical specimens. J Clin Microbiol 1996; 34: 2236–2239.
  • Rohner, P., Ninet, B., Metral, C., Emler, S. and Auckenthaler, R. Evaluation of the MB/BacT system in comparison to the BACTEC 460 system and solid media for isolation of myco- bacteria from clinical specimens. J Clin Microbiol 1997; 35: 3127–3131.
  • Centers for Disease Control and Prevention (CDC) Essential components of a tuberculosis prevention and control pro- gram. Morbid Mortal Weekly Rep 1995; 44: 11-16.
  • Rüsch-Gerdes S, Ebraihmzadeh A, Elliott LB, Hanna BA, Mor- gan MA, Novak S. Multicenter evaluation of the BACTEC MGIT 960 system compared to the BACTEC 460 TB and solid media for recovery of mycobacteria. In: Abstracts of the 19th Congress of the European Society of Mycobacteriology. Rev Port Pneumol 1998; 4: 346.
  • Scarparo C, Ricordi P, Ruggiero G, Piccoli P. Evaluation of the fully automated BACTEC MGIT 960 system for testing sus- ceptibility of Mycobacterium tuberculosis to pyrazinamide, streptomycin, isoniazid, rifampin, and ethambutol and com- parison with the radiometric BACTEC 460TB method.J Clin Microbiol. 2004; 42:1109-1114.
  • Kocagoz T, Alp A, Albay A. A new rapid non-radioactive me- dium for culturing mycobacteria, that also enables visual dif- ferentiation of mycobacterial growth from contamination (Ab- stract) . Los Angeles, CA: American Society of Microbiology General Meeting. May, 21-25, 2000.
  • Ercis S, Alp A, Hascelik G, Kocagoz T. Comparison of Dio- TK rapid mycobacterium culture system with BACTEC 460 TB system and LJ medium in diagnosis of tuberculosis and detection of susceptibility of antituberculosis drugs (Abstract) Abant, Bolu, Turkey; 4th National Mycobacterium Sympo- sium, 2002: 180-181.
  • Bicmen C, Coskun M, Senol G, Florat N, Kocagoz T. Com- parison of Dio-TK and LJ media for primary culture of my- cobacteria: a preliminary study for a new medium (Abstract) Glasgow, UK: 13th ECCMID, May, 2003.
  • Baylan O, Kisa O, Albay A, L. Doganci. Evaluation of a new automated, rapid, colorimetric culture system using solid me- dium for laboratory diagnosis of tuberculosis and determina- tion of anti-tuberculosis drug susceptibility. Int J Tuberc Lung Dis 2004; 8:772–777.
  • Solis LA, Shin SS, Han LL, Llanos F, Stowell M, Sloutsky A. Validation of a rapid method for detection of M.tuberculosis resistance to isoniazid and rifampin in Lima, Peru.Int J Tu- berc Lung Dis 2005; 9: 760-764.
  • Negi SS, Khan SF, Gupta S, Pasha ST, Khare S, Lal S. Com- parison of the conventional diag-nostic modalities, bactec culture and polymerase chain reaction test for diagnosis of tuberculosis. Indian J Med Microbiol 2005;23:29-33.
  • Ani A, Okpe S, Akambi M, Ejelionu E, Yakubu B, Owolodun O, Ekeh P, Oche A, Tyen D, Idoko J. Comparison of a DNA based PCR method with conventional methods for the detec- tion of M.tuberculosis in Jos, Nigeria. J Infect Dev Countries 2009; 3: 470-475.
There are 14 citations in total.

Details

Primary Language English
Journal Section ART
Authors

Mustafa Altındiş This is me

Zafer Çetinkaya This is me

Raike Kalaycı This is me

İhsan H. Çiftçi This is me

Alpaslan Arslan This is me

Orhan C. Aktepe This is me

Publication Date March 1, 2011
Published in Issue Year 2011 Volume: 1 Issue: 01

Cite

APA Altındiş, M., Çetinkaya, Z., Kalaycı, R., Çiftçi, İ. H., et al. (2011). Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs. Journal of Microbiology and Infectious Diseases, 1(01), 5-9. https://doi.org/10.5799/ahinjs.02.2011.01.0002
AMA Altındiş M, Çetinkaya Z, Kalaycı R, Çiftçi İH, Arslan A, Aktepe OC. Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs. J Microbil Infect Dis. March 2011;1(01):5-9. doi:10.5799/ahinjs.02.2011.01.0002
Chicago Altındiş, Mustafa, Zafer Çetinkaya, Raike Kalaycı, İhsan H. Çiftçi, Alpaslan Arslan, and Orhan C. Aktepe. “Detection of Mycobacterium Isolates With Different Methods and Their Resistance Ratios Against Anti-Tuberculosis Drugs”. Journal of Microbiology and Infectious Diseases 1, no. 01 (March 2011): 5-9. https://doi.org/10.5799/ahinjs.02.2011.01.0002.
EndNote Altındiş M, Çetinkaya Z, Kalaycı R, Çiftçi İH, Arslan A, Aktepe OC (March 1, 2011) Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs. Journal of Microbiology and Infectious Diseases 1 01 5–9.
IEEE M. Altındiş, Z. Çetinkaya, R. Kalaycı, İ. H. Çiftçi, A. Arslan, and O. C. Aktepe, “Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs”, J Microbil Infect Dis, vol. 1, no. 01, pp. 5–9, 2011, doi: 10.5799/ahinjs.02.2011.01.0002.
ISNAD Altındiş, Mustafa et al. “Detection of Mycobacterium Isolates With Different Methods and Their Resistance Ratios Against Anti-Tuberculosis Drugs”. Journal of Microbiology and Infectious Diseases 1/01 (March 2011), 5-9. https://doi.org/10.5799/ahinjs.02.2011.01.0002.
JAMA Altındiş M, Çetinkaya Z, Kalaycı R, Çiftçi İH, Arslan A, Aktepe OC. Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs. J Microbil Infect Dis. 2011;1:5–9.
MLA Altındiş, Mustafa et al. “Detection of Mycobacterium Isolates With Different Methods and Their Resistance Ratios Against Anti-Tuberculosis Drugs”. Journal of Microbiology and Infectious Diseases, vol. 1, no. 01, 2011, pp. 5-9, doi:10.5799/ahinjs.02.2011.01.0002.
Vancouver Altındiş M, Çetinkaya Z, Kalaycı R, Çiftçi İH, Arslan A, Aktepe OC. Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs. J Microbil Infect Dis. 2011;1(01):5-9.