İskenderun Körfezi'ndeki (Kuzeydoğu Akdeniz) Kahverengi ve Kırmızı Makroalglerin Temel Besin Maddesi ve Biyoaktif Bileşiklerindeki Mevsimsel Değişimler

Abstract

Kuzeydoğu Akdeniz'de (İskenderun Körfezi) dağılım gösteren üç kahverengi (Dictyota dichotoma, Padina pavonica, Stypopodium schimperi) ve bir kırmızı makroalgin (Jania rubens) temel besin maddesi ve biyoaktif bileşikleri (toplam fenolik, flavonoid, klorofil-a ve toplam karotenoid içerikleri) üç örnekleme istasyonunda mevsimsel olarak incelenmiştir. İncelenen tüm parametrelerin mevsimsel değişimler gösterdiği belirlenmiştir. En yüksek kül içeriği ilkbaharda J. rubens türünde (%77.7) bulunmuştur. Sonuçlar, bu türün zengin bir mineral kaynağı olduğunu göstermiştir. D. dichotoma en yüksek ham protein içeriğine, S. schimperi ise en fazla lipit içeriğine sahip tür olmuştur. Makroalglerde fenolik madde içeriği 34.6 ile 107.0 mg GAE/g kuru ağ. arasında değişmiştir. Toplam fenolik madde miktarı yazın S. schimperi türünde en yüksek düzeye ulaşırken, en düşük değer ilkbaharda P. pavonica türünde bulunmuştur. Flavonoid içerikleri (9.05-10.6 mg QE/g kuru ağ.) kahverengi deniz yosunlarında kırmızı deniz yosunundan daha yüksek bulunmuştur. Klorofil-a ve karotenoid içeriği ise D. dichotoma türünde sonbahar mevsiminde en yüksek düzeylerde (sırasıyla 4.53 ve 2.83 mg/g) bulunmuştur. Sonuçlar, incelenen makroalg türlerinin biyokimyasal kompozisyonunun türe, lokaliteye ve mevsimlere bağlı olarak belirgin değişimler gösterebileceğini ortaya koymuştur.

Keywords

• Makroalg
• Temel besin maddesi kompozisyonu
• Biyoaktif bileşikler
• Fenolik
• Flavonoid

Seasonal Changes in Proximate and Bioactive Compounds of Brown and Red Seaweeds from İskenderun Bay, the North-Eastern Mediterranean Sea

Abstract

Proximate and bioactive compounds (total phenolic, flavonoid, chlorophyll-a and total carotenoid contents) of three brown seaweeds (Dictyota dichotoma, Padina pavonica, Stypopodium schimperi) and a red seaweed (Jania rubens) from the north-eastern Mediterranean Sea (İskenderun Bay) were investigated seasonally at three sampling sites. Seasonal variations were found for all of the parameters studied. The highest ash content was in J. rubens (77.7%) in the spring. The results showed that J. rubens is a rich source with respect to mineral content. D. dichotoma had the highest crude protein content, whereas S. schimperi contained the most lipids. Phenolics ranged between 34.6 - 107.0 mg GAE/g dw. The highest total phenolics were found in S. schimperi in the summer, and the lowest in P. pavonica in the spring. The flavonoid contents (9.05-10.6 mg QE/g dw) were higher in brown seaweeds than that in the red seaweed. Moreover, chlorophyll-a and carotenoids levels were highest in D. dichotoma (4.53 and 2.83 mg/g, respectively) during the autumn. The results revealed that the biochemical composition of the examined seaweeds showed significant changes depending on the species, location and seasons.

Keywords

• Seaweed
• Proximate composition
• Bioactive compounds
• Phenolics
• Flavonoids

References

1. AOAC (1990). Official Methods of Analysis of AOAC International.15th ed. Association of Official Analytical Chemists, Method 950.46, Moisture in Meat. Washington, DC: USA.
2. AOAC (1998a). Official Methods of Analysis of AOAC International. Association of Official Analytical Chemists. Method 938.08, Ash of seafood: Fish and other marine products. Gaithersburg, MD: USA.
3. AOAC (1998b). Official Methods of Analysis of AOAC International. Association of Official Analytical Chemists. Method 955.04, Nitrogen (Total) in Seafood. Fish and Other Marine Products. Gaithersburg, MD: USA.
4. Arnon, D.I. (1949). Copper enzymes in isolated chloroplast, polyphenol oxidase in Beta Vulgarise, Plant Physiology, 2, 1-15.
5. Aysel, V., Erdugan, H.  Okudan, E.Ş. (2006a). Marine algae and seagrasses of Adana (Mediterranean, Turkey), Journal of Black Sea/Mediterranean Environment, 12, 35-57.
6. Aysel, V., Erdugan, H.  Okudan, E.Ş. (2006b). Marine algae and seagrasses of Hatay Mediterranean, Turkey), Journal of Black Sea/Mediterranean Environment, 12, 159-179.
7. Bligh, E.G.  Dyer, W.J. (1959). A rapid method of total lipid extraction and purification, Canadian Journal Biochemistry Physiology, 37, 911-917.
8. Boominathan, M.  Mahesh, A. (2015). Seaweed carotenoids for cancer therapeutics. In: Handbook of Anticancer Drugs from Marine Origin (edited by S.K. Kim) (pp.185-203). Cham, Switzerland: Springer.

9. Caf, F., Şen Özdemir, N., Yılmaz, Ö., Durucan, F. & Ak, İ. (2019). Fatty acid and lipophilic vitamin composition of seaweeds from Antalya and Çanakkale (Turkey), Grasas Y Aceites 70 (3), 1-7. doi:10.3989/gya.0704182
10. Chakraborty, S.  Bhattacharya, T. (2012). Nutrient composition of marine benthic algae found in the Gulf of Kutch coastline, Gujarat India, Journal Algal Biomass, 3, 32-38.
11. Chauhan, V.  Chauhan, A. (2006). Oxidative stress in Alzheimer’s disease, Pathophysiology, 13, 195-208. doi.org/10.1016/j.pathophys.2006.05.004
12. Chang, C.C., Yang, M.H., Wen, H.M.  Chern, J.C. (2002). Estimation of total flavonoid content in propolis by two complementary colorimetric methods, Journal of Food Drug Analysis, 10, 178-182. doi.org/10.38212/2224-6614.2748 Chinnadurai, S., Karthik, G., Chermapandi P.  Hemalatha, A. (2013). Estimation of Major Pigment Content in Seaweeds Collected from Pondicherry Coast, The Experiment, 9 (1), 522-525.
13. Connan, S., Deslandes, E.  Gall, E.A. (2007). Influence of day–night and tidal cycles on phenol content and antioxidant capacity in three temperate intertidal brown seaweeds, Journal of Experimental Marine Biology and Ecology, 349, 359-369. doi.org/10.1016/j.jembe.2007.05.028
14. Dixit, D. & Reddy, C. R. K. (2017). Non-Targeted Secondary Metabolite Profile Study for Deciphering the Cosmeceutical Potential of Red Marine Macro Alga Jania rubens-An LCMS-Based Approach" Cosmetics, 4(4), 45. doi.org/10.3390/cosmetics4040045
15. Etemadian, Y., Shabanpour, B., Ghaemi, V.  Kordjazi.M. (2017). Compare the chlorophyll amount in three brown algae species of the Persian Gulf by using three solvents and applying two formulas, International Journal of Biochemistry, Biophysics & Molecular Biology, 2 (6), 77-79. doi: 10.11648/j.ijbbmb.20170206.14
16. Fleurence, J. (1999). Seaweed proteins: biochemical, nutritional aspects and potential uses, Trends in Food Science Technology, 10, 25-28. doi.org/10.1016/S0924-2244(99)00015-1
17. Fox, J.  Weisberg, S. (2011). An R Companion to Applied Regression. Second Edition. Thousand Oaks CA: Sage URL: http://socserv.socsci.mcmaster.ca/jfox/Books/
18. Gámez-Meza, N., Noriega-Rodriguez, J.A., Medina-Juarez, L.A. et al. (1999). Antioxidant activity in soybean oil of extracts from Thompson grape bagasse, Journal of American Oil Chemists' Society, 76, 1445-1447. doi.org/10.1007/s11746-999-0182-4
19. Godinez-Ortega, J.L., Snooeijis, P., Robledo, D., Freile-Pelegrin, Y.  Pedersen M. (2008). Growth and pigment composition in the red alga Halymenia floresii cultured under different light qualities, Journal of Applied Phycology, 20, 253-260. doi.org/10.1007%2Fs10811-007-9241-0
20. Güner, A.  Yavasoglu, N.K. (2018). Evaluation of antioxidant, antimicrobial and antimutagenic activity with irritation effects of Ceramium rubrum (Red Algae) extract, International Journal of Secondary Metabolites, 5, 279-287. doi.org/10.21448/ijsm.432654
21. Haroon, A., Szaniawska, A., Normant, M.  Janas, U. (2000). The biochemical composition of Enteromorpha spp. from the Gulf of Gdansk coast on the southern Baltic Sea, Oceanologia, 116/117, 513-516.
22. Hothorn, T., Bretz, F.  Westfall, P. (2008). Simultaneous inference in general parametric Models, Biometrical Journal, 50, 346-363. doi.org/10.1002/bimj.200810425
23. İrkin, L.C. & Erdugan, H. (2016). Seasonal variation in ash, lipid and protein contents of Scytosiphon lomentaria Lyngbye and Palisada perforata Bory de Saint-Vincent along Çanakkale Strait (Dardanelles), Turkey, Marine Science and Technology Bulletin, 4 (2), 1-4.
24. İrkin, L.C.  Erduğan, H. (2017). Investigation of seasonal variations in biochemical composition of some red algae distributed in the Strait of Çanakkale (Dardanelles), Turkey, Archive of Applied Science Research, 9, 1-8.
25. Kalasariya, H.S., Yadav, V.K., Yadav, K.K., Tirth, V., Algahtani, A., Islam, S., Gupta, N.  Jeon, B-H. (2021). Seaweed-based molecules and their potential biological activities: An eco-sustainablecosmetics, Molecules, 26, 5313. doi.org/10.3390/molecules26175313
26. Kirk, J.T.O.  Allen, R.L. (1965). Dependence of chloroplast pigments synthesis on protein synthetic effect on actilion, Biochemical and Biophysical Research, 27, 523-530.
27. Kostetsky, E.Y., Goncharova, S.N., Sanina, N.M.  Shnyrov, V.L. (2004). Season influence on lipid composition of marine macrophytes, Botanica Marina, 47, 134-139. doi.org/10.1515/BOT.2004.013
28. Kumar, C.S., Ganesan, P., Suresh, P.V.  Bhaskar N. (2008). Seaweeds as a source of nutritionally beneficial compounds - A review, Journal of Food Science and Technology, 45, 1-13.
29. Kumar, M., Gupta, V., Kumari, P., Reddy, C.R.K.  Jha, B. (2011). Assessment of nutrient composition and antioxidant potential of Caulerpaceae seaweeds, Journal of Food Composition Analysis, 24, 270-278. doi.org/10.1016/j.jfca.2010.07.007
30. Machu, L., Misurcova, L., Ambrozova, J.V., Orsavova, J., Mlcek, J., Sochor, J., Jurikova, T. (2015). Phenolic content and antioxidant capacity in algal food products, Molecules, 20, 1118-1133. doi.org/10.3390/molecules20011118
31. Marinho, G.S., Sørensen, A.D.M.; Safafar, H., Pedersen, A.H., Holdt, S.L. (2019). Antioxidant content and activity of the seaweed Saccharina latissima: A seasonal perspective. Journal of Applied Phycology, 31, 1343–1354. doi: 10.1007/s10811-018-1650-8
32. Marsham, S., Scott, G.W.  Tobin, M.L. (2007). Comparison of nutritive chemistry of a range of temperate seaweeds, Food Chemistry, 100, 1331-1336. doi.org/10.1016/j.foodchem.2005.11.029
33. Matanjun, P., Mohamed, S., Mustapha, N.M., Muhammad, K.  Ming, C.H. (2008). Antioxidant activities and phenolics content of eight species of seaweeds from north Borneo, Journal of Applied Phycology, 20, 367-373.doi:10.1007/s10811-007-9264-6
34. Necchi, O.  Zucchi, M.R. (2001). Effects of temperature, irradiance and photoperiod on growth and pigment content in some freshwater red algae in culture, Phycological Research, 49, 103-114. doi.org/10.1111/j.1440-1835.2001.tb00240.x
35. Nedumaran, T.  Arulbalachandran, D. (2015). Seaweeds: A Promising Source for Sustainable Development. In: Thangavel P, Sridevi G (Eds.) Environmental Sustainability (pp. 65-88).
36. Nelson, M.M., Phleger, C.F.  Nichols, P.D. (2002). Seasonal lipid composition in macroalgae of the Northeastern Pacific Ocean, Botanica Marina, 45, 58-65. doi.org/10.1515/BOT.2002.007
37. Palanivelu, A., Darsis, A.  Arunkumar, K. (2012). Nutraceutical values of seaweeds found along the Coast of Thondi (Palk Bay, India) with specific investigation on fatty acids methyl esters through GC/MS, Journal of Green Bioenergy, 1, 3-18.
38. Peñalver, R., Lorenzo, J.M., Ros, G., Amarowicz, R., Pateiro, M.  Nieto, G. (2020). Seaweeds as a functional ingredient for a healthy diet, Marine Drugs, 18, 1-27. doi.org/10.3390/md18060301
39. Parthiban, C., Saranya, C., Gırija, K., Hemalatha, A., Suresh, M.  Anantharaman, P. (2013). Biochemical composition of some selected seaweeds from Tuticorin coast, Advances in Applied Science Research, 4, 362-366.
40. Polat, S.  Özoğul, Y. (2008). Biochemical composition of some red and Brown macroalgae from the Northeastern Mediterranean Sea. International Journal of Food Science and Nutrition, 59, 566-572. doi.org/10.1080/09637480701446524
41. Polat, S.  Özoğul, Y. (2009). Fatty acid, mineral and proximate composition of some seaweeds from the northeastern Mediterranean coast, Italian Journal Food Science, 21, 317-324.
42. Polat, S., Özoğul Y.  Küley Boğa, E. (2012). Protein, lipid and fatty acid composition of some brown and red seaweeds from the coast of İskenderun Bay (Northeastern Mediterranean), Journal of FisheriesSciences.com, 6, 107-113. doi: 10.3153/jfscom.2012014
43. Polat, S.  Özoğul, Y. (2013). Seasonal proximate and fatty acid variations of some seaweeds from the northeastern Mediterranean coast, Oceanologia, 55, 375-391. doi.org/10.5697/oc.55-2.375
44. R Core Team (2018). R: A language and environment for statistical computing. R Foundation for Statistical Computing Vienna Austria. https://www.R-project.org/
45. Radha, P. (2018). Proximate analysis and mineral composition of seaweeds of Manamelkudi coast, Pudukkottai District, India, International Journal of Current Microbiology and Applied Sciences, 7(8): 3121-3128. doi.org/10.20546/ijcmas.2018.708.333
46. Ratana-arporn, P.A.  Chirapart, A. (2006). Nutritional evaluation of tropical green seaweeds Caulerpa lentillifera and Ulva reticulate, Kasetsart Journal Natural Science, 40, 75-83.
47. Renaud, S.M.  Luong-Van, J.T. (2006). Seasonal variation in the chemical composition of tropical Australian marine macroalgae, Journal of Applied Phycology, 18, 381-387. doi.org/10.1007/s10811-006-9034-x
48. Rupérez, P. (2002). Mineral content of edible marine seaweeds, Food Chemistry, 79, 23-26. doi.org/10.1016/S0308-8146(02)00171-1
49. Sahayaraj, K., Asharaja, A.C., Rajesh, S.  Martin Rathi, J.A. (2014). Qualitative and quantitative profiles of secondary metabolites of chosen Chlorophyta and Ochrophyta from Gulf of Mannor, Cahiers de Biologie Marine, 55, 69-76.
50. Saygılı, E. I., Naz, M., Okudan, E. S., Çetin, Z., Benlier, N., Öğüt, E., Güngör, M., Bakır, S.B., Karadeniz, P.G., Veziroglu, S., Gülses, A., Depci, T., Aktas, O.C.  Sayın, S. (2022). The determination of the molecular weight profiles and biochemical compositions eight macroalgae species from Turkey, International Aquatic Research, 14(2), 117-125. doi:10.22034/iar.2022.1949245.1226
51. Sukalyan, C.  Santra, S.C. (2008). Biochemical composition of eight benthic algae collected from Sunderban, Indian Journal of Marine Sciences, 37, 329-332.
52. Tabarsa, M., Rezai, M., Ramezanpour, Z., Waaland, J.R.  Rabirei, R. (2012). Fatty acids, amino acids, mineral contents and proximate composition of some brown seaweeds, Journal of Phycology, 48, 285-292. doi.org/10.1111/j.1529-8817.2012.01122.x
53. Taşkın, E. (2014). Comparison of the brown algal diversity between four sea coasts of Turkey, Journal of Academic Documents for Fisheries and Aquaculture. 3, 145-150.
54. Turan, F., Özgun, S., Sayın, S.  Özyılmaz, G. (2015). Biochemical composition of some red and green seaweeds from Iskenderun Bay, the northeastern Mediterranean coast of Turkey, Journal of Black Sea/Mediterranean Environment, 21, 239-249.
55. Thirumaran, G., Manivannan, K., Karthikai, D.G., Ananthamaran, P.  Balasubramanian, T. (2009). Photosynthetic pigments of different colour strains of the cultured seaweeds Kappaphycus alvarezii (Doty) ex P.Silva in Valler Estuary, Academic Journal of Plant Science, 2, 150-153.
56. Wang, B.G., Zhang, W.W., Duan, X.J.  Li, X.M. (2009). In vitro antioxidative activities of extract and semi-purified fractions of the marine red alga, Rhodomela confervoides (Rhodomelaceae), Food Chemistry, 113, 1101-1105. doi.org/10.1016/j.foodchem.2008.08.078
57. Yeşilova, K., Balkis, N. & Taşkın, E. (2017). Seasonal investigation of the protein, carbohydrate and lipid contens of dominant macroalgae on the western coast of the Black Sea. Fresenius Environmental Bulletin, 26 (1), 46-55.
58. Yılmaz, M. , Türker, G. & Ak, İ. (2021). The Effect of Different Solvents on Antioxidant Properties of Gongolaria barbata (Phaeophyceae) . Çanakkale Onsekiz Mart University Journal of Marine Sciences and Fisheries, 4(2), 197-201 . doi: 10.46384/jmsf.1021387
59. Zeileis, A. (2004). Econometric computing with HC and HAC covariance matrix estimators, Journal of Statistical Software, 11, 1-17. doi.org/10.18637/jss.v011.i10