Otolith Shape Analysis and Otolith Morphometry of Bogue Boops boops (Linnaeus, 1758) in the Aegean Sea and the Marmara Sea

Abstract

In the present study, otolith shape indices and some otolith morphometric characteristics in the left and right sagittal otoliths were identified and compared for Boops boops in the Aegean Sea and the Marmara Sea. Otoliths of Boops boops from 244 individuals in the North of Aegean Sea and 188 individuals from the Southwest of Marmara Sea were analyzed during the 2024 fishing season. The total length-otolith dimensions relationships were evaluated, the best fit was obtained between total length and otolith perimeter in the Marmara Sea and Aegean Sea, while the least fit was obtained among total length and otolith area of right otoliths, total length and otolith length of left otoliths in the Aegean Sea and Marmara Sea. Some similarities of Boops boops stocks were found with shape indices and otolith morphometry between the Aegean Sea and Marmara Sea. The otolith area and circularity of the left otolith, roundness and rectangularity of the right otolith, form factor of left and right otolith were significantly different (p<0.05). Principle Component Analysis may explain these differences as arising from variations in the roundness, otolith area and otolith width. Also, Canonical Discriminant Analysis demonstrated significant discrimination of otoliths between the Aegean Sea and the Marmara Sea (p<0.05), and 56.6% of the originally grouped cases were correctly classified. Based on the results of this study, two stocks of Boops boops in the North of Aegean Sea and the Southwest of Marmara Sea might be different. Howewer, more detailed studies are required the determine whether this species constitues the same stock in these areas.

Keywords

• Otolith shape indices
• Stock discrimination
• Çanakkale Strait
• Otolith dimensions
• Sagittal otolith

Ege Denizi ve Marmara Denizi'ndeki Kupez’in Boops boops (Linnaeus, 1758) Otolit Şekil Analizi ve Otolit Morfometrisi

Abstract

Bu çalışmada, Ege Denizi ve Marmara Denizi'nde Boops boops'un otolit şekil indeksleri ile bazı otolit morfometrisi özellikleri sağ ve sol sagittal otolitlerinde tanımlanmış ve karşılaştırılmıştır. 2024 balıkçılık sezonunda Ege Denizi’nin kuzeyinden 244 birey ile Marmara Denizi’nin güneybatısından 188 bireyin otolitleri analiz edilmiştir. Toplam boy-otolit boyutları ilişkileri değerlendirildiğinde, Marmara Denizi ve Ege Denizi'nde toplam boy ile otolit çevresi arasında en iyi uyum sağlanırken, Marmara Denizi ve Ege Denizi'nde toplam boy ile sağ otolitlerin otolit alanı ile toplam boy ile sol otolitlerin otolit boyu arasında en düşük uyum sağlanmıştır. Boops boops stokları arasında Ege Denizi ve Marmara Denizi’nde otolit şekil indeksleri ve otolit morfometrisi açısından bazı benzerlikler bulunmuştur. Sol otolitin alanı ve daireselliği, sağ otolitin yuvarlaklığı ve dikdörtgenselliği, sol ve sağ otolitin form faktörü açısından anlamlı farklılıklar göstermiştir (p<0,05). Temel Bileşen Analizi, bu denizlerdeki farklılıkların yuvarlaklık, otolit alanı ve otolit genişliğinden kaynaklandığını açıklayabilir. Ayrıca, Kanonik Ayırım Analizi, Ege Denizi ile Marmara Denizi arasında otolitlerin anlamlı şekilde ayırt edilebildiğini (p<0,05) ve gruplandırılan örneklerin %56,6'sının doğru şekilde sınıflandırıldığını ortaya koymuştur. Bu çalışmanın sonuçlarına göre, Ege Denizi’nin kuzeyindeki ve Marmara Denizi’nin güneybatısındaki iki Boops boops stoğunun farklı olabileceği düşünülmektedir. Fakat bu türün, bu denizlerde aynı stok olup olmadığının incelenmesini içeren daha ayrıntılı çalışmalar gerekmektedir.

Keywords

• otolit şekil indeksi
• Stok ayrımı
• Çanakkale Boğazı
• Otolit boyutları
• sagittal otolit

References

1. Bal, H., Yanik, T., & Turker, D. (2021). Assessment of morphological variation between stocks of bluefish, Pomatomus saltatrix (Actinopterygii, Perciformes, Pomatomidae), in the Aegean Sea, Black Sea, and Sea of Marmara. Acta Ichthyologica et Piscatoria, 51(1), 85–94. https://doi.org/10.3897/aiep.51.63319
2. Bauchot, M. L., & Hureau, J. C. (1986). Sparidae. In P. J. P. Whitehead, M. L. Bauchot, J. C. Hureau, J. Nielsen & E. Tortonese (Eds.), Fishes of the North-eastern Atlantic and the Mediterranean. Volume 2. (pp. 883-907). Paris: UNESCO.
3. Ben Labidi, M., Mejri, M., Shahin, A. A. A. B., Quignard, J. P., Trabelsi, M., & Ben Faleh, A. (2020). Stock discrimination of the bogue, Boops boops (Actinopterygii: Perciformes: Sparidae), from two Tunisian marine stations using the otolith shape. Acta Ichthyologica et Piscatoria, 50(4), 413-422. https://doi.org/10.3750/AIEP/02978
4. Campana, S. E., & Casselman, J. M. (1993). Stock discrimination using otolith shape analysis. Canadian Journal of Fisheries and Aquatic Sciences, 50(5), 1062-1083. https://doi.org/10.1139/f93-123
5. Cicek, E., Avsar, D., Yeldan, H., & Manasırlı, M. (2021). Otoliths atlas of 77 fish species from the Iskenderun Bay, Northeastern Mediterranean Sea. Fishtaxa-Journal of Fish Taxonomy, 19, 9-55.
6. Durrani, O., Bal, H., Battal, Z. S., & Seyhan, K. (2022). Using otolith and body shape to discriminate between stocks of European anchovy (Engraulidae: Engraulis encrasicolus) from the Aegean, Marmara and Black Seas. Journal of Fish Biology, 101(6), 1452-1465. https://doi.org/10.1111/jfb.15216
7. Hammer, D., Harper, D. A. T., & Ryan, P. D. (2001). PAST: Palaeontological statistics software package for education and data analysis. Palaeontologia Electronica, 4(1): 9.
8. IBM Corp, (2017). IBM SPSS Statistics for Windows, Version 25.0. Armonk, NY, USA.

9. Ider, D., Ramdane, Z., Mahe, K., Duffour, J. L., Bacha, M., & Amara, R. (2017). Use of otolith-shape analysis for stock discrimination of Boops boops along the Algerian coast (southwestern Mediterranean Sea). African Journal of Marine Science, 39(3), 251-258. https://doi.org/10.2989/1814232X.2017.1363817
10. Ozturk, I. (2021). In the light of scientific information the Marmara Sea and the Turkish Straits System. Turkish Academy of Sciences, TDV Yayın Matbaacılık Ankara, Science and Thought Series No: 31, 750 pcs.
11. Saygılı, B., Ismen, A., & Ihsanoglu, M. A. (2016). Differences in the otolit shape between The Marmara and Northern Aegean Sea Stocks of Blotched Picarel (Spicara maena Linnaeus, 1758). Journal of Black Sea/Mediterranean Environment, 22(2), 137-148.
12. Schade, F. M., Weist, P., & Krumme, U. (2019). Evaluation of four stock discrimination methods to assign individuals from mixed-stock fisheries using genetically validated baseline samples. Marine Ecology Progress Series, 627, 125-139. https://doi.org/10.3354/meps13061
13. Scherrer, B. (1984). Biostatistique. Morin, Montre´al, Paris.
14. Schultz-Mirbach, T., & Reichenbacher, B. (2006). Reconstruction of Oligocene and Neogene freshwater fish faunas: an actualistic study on cypriniform otoliths. Acta Palaeontologica Polonica, 51(2), 283-304.
15. Mille, T., Mahé, K., Villanueva, M. C., de Pontual. H., & Ernande, B. (2015). Sagittal otolith morphogenesis asymmetry in marine fishes. Journal of Fish Biology, 87, 646-663. https://doi.org/10.1111/jfb.12746
16. Morales-Nin, B., Pérez-Mayol, S., MacKenzie, K., Catalán, I. A., Palmer, M. , Kersaudy, T., & Mahé, K. (2022). European hake (Merluccius merluccius) stock structure in the Mediterranean as assessed by otolith shape and microchemistry. Fisheries Research, 254, 106419. https://doi.org/10.1016/j.fishres.2022.106419
17. Ponton, D. (2006). Is geometric morphometrics efficient for comparing otolith shape of different fish species? Journal of Morphology, 267, 750-757. https://doi.org/10.1002/jmor.10439
18. Popper, A. N., & Lu, Z. (2000). Structure–function relationships in fish otolith organs. Fisheries Research, 46(1-3), 15-25. https://doi.org/10.1016/S0165-7836(00)00129-6
19. Popper, A. N., Ramcharitar, J., & Campana, S. E. (2005). Why otoliths? Insights from inner ear physiology and fisheries biology. Marine and Freshwater Research, 56(5), 497-504. https://doi.org/10.1071/MF04267
20. Tuset, V. M., Lombarte, A., Gonzalez, J. A., Pertusa, J. F., & Lorente, M. J. (2003a). Comparative morphology of the sagittal otolith in Serranus spp. Journal of Fish Biology, 63, 1491–1504. https://doi.org/10.1046/j.1095-8649.2003.00262.x.
21. Tuset, V. M., Lozano, I. J., González, A., Pertusa, J. F., & García-Díaz, M. M. (2003b). Shape indices to identify regional differences in otolith morphology of comber, Serranus cabrilla (L., 1758). Journal of Applied Ichthyology, 19, 88-93. https://doi.org/10.1046/j.1439-0426.2003.00344.x
22. Tuset, V. M., Farré, M., Otero-Ferrer, J. L., Vilar, A., Morales-Nin, B., & Lombarte, A. (2016). Testing otolith morphology for measuring marine fish biodiversity. Marine and Freshwater Research, 67, 1037–1048. https://doi.org/10.1071/MF15052
23. TUIK, (2024). Turkish Statistical Institute, Fishery Statistic. (Accessed date: 15.12.2024), https://biruni.tuik.gov.tr/medas/?locale=tr
24. Van Damme, A., Tuset, V. M., Frédérich, B., Parmentier, E., Fatira, E., Schulz-Mirbach, T., Medeiros, A. P. M., Betancjur-R, T., & Lombarte, A. (2024). The multifaceted diversification of the sagitta otolith across the fish tree of life. Biological Journal of the Linnean Society, 143(2), blae085. https://doi.org/10.1093/biolinnean/blae085
25. Vignon, M., Morat, F., Galzin, R., & Sasal, P. (2008). Evidence for spatial limitation of the bluestripe snapper Lutjanus kasmira in French Polynesia from parasite and otolith shape analysis. Journal of Fish Biology, 73(10), 2305-2320. https://doi.org/10.1111/j.1095-8649.2008.02070.x
26. Vignon, M., & Morat, F. (2010). Environmental and genetic determinant of otolith shape revealed by a non-indigenous tropical fish. Marine Ecology Progress Series, 411, 231–241. https://doi.org/10.3354/meps08651
27. Yedier, S., Yalcınkaya, S. K., Turker, D., & Bostancı, D. (2023a). Ecomorphological patterns and shape indices of otoliths in the Pagellus acarne (Actinopterygii, Sparidae) from the Aegean and Marmara Seas. Turkish Journal of Zoology, 47(4), 222-230. https://doi.org/10.55730/1300-0179.3135
28. Yedier, S., Kontas Yalcinkaya, S., Bostanci, D., & Polat, N. (2023b). Morphologic, morphometric and contour shape variations of sagittal otoliths of Lepidorhombus spp. in the Aegean Sea. Anatomia, Histologia, Embryologia, 52(2), 279–288. https://doi.org/10.1111/ahe.12881