Bacterial Infection in Cultured Common Dentex (Dentex dentex, L. 1758)

Year 2019, Volume: 2 Issue: 1, 132 - 138, 05.07.2019

Tülay Akaylı , Çiğdem Ürkü Remziye Yardımcı , Özgür Çanak

Abstract

The aim of this study is the
identification of microbial agents recovered from 8 moribund common dentex (Dentex dentex) samples (140-200 g), determination of their antimicrobial
susceptibilities and description of histopathological changes. Main clinical
external and internal symptoms of bacterial haemorrhagic septicaemia cases such
as fin base and liver haemorrhages, splenomegaly, accumulation of fluid in the
intestines and peritoneal cavity were observed. Bacteriological samples of
liver, kidney and spleen were streaked onto Marine Agar and Tryptic Soy Agar
plates containing %1,5 NaCl and incubated at 22°C for 48h. Gram-positive
non-motile cocci and Gram-negative motile bacillus were isolated from visceral
organs. According to morphological and biochemical characteristics, isolates
were identified as Micrococcus luteus
and Vibrio sp. The Kirby-Bauer disk
diffusion method was also performed using multidisc and all isolates were
determined to be sensitive against kanamycin, oxytetracycline and flumequine.
In the infected fish, general cell degenerations and necrosis in various
tissues, a remarkable increase in the goblet cells of the intestines, hepatic
lipidosis in the liver, hyperplasia in the gills and the presence of
melanomacrophage centres in various tissues were demonstrated. As a result,
here, a mixed infection in moribund fish samples was described and this is the
first report on the histopathological changes of M. luteus infection in a marine fish species. This bacterium was
detected as a possible pathogen of common dentex with various external, internal and histopathological
damages.

Keywords

fish diseases, histopathology, common dentex, Micrococcus luteus

Supporting Institution

İstanbul Ünivefrsitesi Bilimsel Araştırma Projeleri Destek Birimi

Project Number

2637

Thanks

This study was supported by the Istanbul University Research Projects Found with the project number 2637

Kültür Sinarit (Dentex dentex, L. 1758) Balıklarında Bakteriyel Enfeksiyon

Abstract

Bu çalışmada toplam 8 adet hasta sinarit (Dentex dentex) (140-200g) balığının
hastalık etkenlerinin identifikasyonu ve antimikrobiyal duyarlılıkları yanı
sıra balıklarda meydana gelen histopatolojik değişimlerin ortaya çıkartılması
amaçlanmıştır. Hasta balık numunelerinde, yüzgeç diplerinde ve karaciğerde
hemorajiler, dalakta büyüme, bağırsaklar ile periton boşluğunda sıvı birikimi
gibi bakteriyel hemorajik septisemi olgularında görülen iç ve dış semptomlar
gözlemlenmiştir. Bakteriyolojik ekimler enfekte balıkların karaciğer, böbrek ve
dalak gibi iç organlarından %1,5NaCl içeren Triptik Soy Agar’a ve Marine Agar’a
yapılmış ve petri kapları 22°C’de 48 saat inkübe edilmiştir. İç organlardan
Gram-pozitif hareketsiz koklar ve Gram-negatif hareketli basiller izole
edilmiştir. Morfolojik ve biyokimyasal özelliklerine göre izolatlar Micrococcus luteus ve Vibrio sp. olarak identifiye edilmiştir.
Kirby-Bauer disk diffüzyon metodu çoklu disk yöntemi kullanılarak uygulanmış ve
tüm izolatların kanamisin, oksitetrasiklin ve flumekuine karşı duyarlı olduğu
tespit edilmiştir. Enfekte balıkların çeşitli dokularında genel olarak hücre
dejenerasyonu ve nekrozu, bağırsaklarda goblet hücrelerinde gözle görülür bir
artış, karaciğerde hepatik lipidosis, solungaçlarda hiperplazi ve çeşitli dokularda
melanomakrofaj merkezlerinin varlığı gözlenmiştir. Sonuç olarak; bu çalışma ile hasta deniz balığı örneklerinde karma
bir enfeksiyon tanımlanmıştır ve bu bir deniz balığı türünde M. luteus enfeksiyonuna bağlı histopatolojik değişimler üzerine ilk rapordur. Bu
bakterinin, neden olduğu çeşitli dış, iç ve histopatolojik etkileri nedeniyle sinarit
balığının olası bir patojeni
olduğu tespit edilmiştir.

Keywords

Balık hastalıkları, Histopatoloji, Sinarit, Micrococcus luteus

Project Number

2637

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