Investigation of Pesticide Residues in Natural Comb Honey from Tokat Province

Year 2025, Volume: 14 Issue: 2, 181 - 188, 31.08.2025

Yaşar Gülmez , Seher Dilek Koçak

https://doi.org/10.54187/jnrs.1705103

Abstract

Pesticides used in agricultural production pose a threat to bee health and may lead to residue issues in honey. While foraging for food, bees can carry chemicals to the hive from the environment, which may result in pesticide residues in bee products. In this study, 24 samples of natural comb honey collected from beekeepers in various districts of Tokat province, including Almus, Artova, Erbaa, Merkez, Niksar, Reşadiye, Pazar, Turhal, and Zile, were analyzed for 261 different pesticide residues. Natural comb honey samples were preferred to avoid potential pesticide contamination from the foundation comb or beeswax. Pesticide analyses were conducted at the Central Laboratory of Gaziosmanpaşa University in Tokat. In a sample from the Reşadiye district (Re-15), tebuconazole fungicide was detected at 0.025 mg/kg and pirimicarb insecticide at 0.469 mg/kg, both exceeding Maximum Residue Limit (MRL) values. However, all other samples tested had pesticide residues below detectable levels. The results suggest that the natural comb honey produced in Tokat province is safe, and the region is conducive to pesticide-free honey production.

Keywords

Tokat , natural comb honey , pesticide , residue

Project Number

2023/40

References

• B. Polat, M. Özüiçli, H. Çetin, L. Aydın, The effects of pesticide usage on honey bee health and products, Journal of Research in Veterinary Medicine 39 (2) (2020) 128–134.
• Z. Barganska, M. Slebioda, J. Namiesnik, Honeybees and their products: Bioindicators of environmental contamination, Critical Reviews in Environmental Science and Technology 46 (3) (2016) 235–248.
• S. Girotti, S. Ghini, E. Ferri, L. Bolelli, R. Colombo, G. Serra, C. Porrini, S. Sangiorgi, Bioindicators and biomonitoring: Honeybees and hive products as pollution impact assessment tools for the Mediterranean area, Euro-Mediterranean Journal for Environmental Integration 5 (2020) 62.
• G. Sonmez Oskay, G. S. Uygur, D. Oskay, N. Arda, Impact of stress factors internal and external to the hive on honey bees and their reflection on honey bee products: a review, Journal of Apicultural Research 64 (1) (2023) 226–241.
• S. T. O'Neal, T. D. Anderson, J. Y. Wu-Smart, Interactions between pesticides and pathogen susceptibility in honey bees, Current Opinion in Insect Science 26 (2018) 57–62
• K. Kerkich, B. Bouargane, A. El Laghdach, B. Souhail, Y. Kadmi, Recent advances in the extraction, purification and analysis of emerging pesticides in honey products: a review, Journal of Food Composition and Analysis 127 (2024) 105947.
• A. Sharma, K. Pant, D. S. Brar, A. Thakur, V. Nanda, A review on Api-products: current scenario of potential contaminants and their food safety concerns, Food Control 145 (2023) 109499.
• R. Vegh, M. Csoka, M. Zsuzsanna, L. Sipos, Pesticide residues in bee bread, propolis, beeswax and royal jelly – A review of the literature and dietary risk assessment, Food and Chemical Toxicology 176 (2023) 113806.
• F. Sanchez-Bayo, K. Goka, Pesticide residues and bees – A risk assessment, PLoS One 9 (4) (2014) e94482.
• F. Sánchez-Bayo, D. Goulson, F. Pennacchio, F. Nazzi, K. Goka, N. Desneux, Are bee diseases linked to pesticides? — A brief review, Environment International 89–90 (2016) 7–11.
• Y. El-Nahhal, Pesticide residues in honey and their potential reproductive toxicity, Science of the Total Environment 741 (2020) 139953.
• C. A. Mullin, M. Frazier, J. L. Frazier, S. Ashcraft, R. Simonds, D. Vanengelsdorp, J. S. Pettis, High levels of miticides and agrochemicals in North American apiaries: implications for honey bee health, PloS One 5 (3) (2010) e9754.
• Official Gazette 31107, Turkish Food Codex Honey Communiqué (2020), https://www.resmigazete.gov.tr/eskiler/2020/04/20200422-13.htm, Accessed 23 May 2025.
• M. S. Seğmenoğlu, E. Baydan, Medicine residues and contaminants found in honey, Journal of Adana Veterinary Control Institute 2 (2012) 24–28.
• A. Yelaldı, K. Kara, T. Balkan, Investigation of insecticide residues in fig and health rist assessment, Turkish Journal of Entomology 48 (3) (2024) 319–326.
• World Health Organization, The WHO Recommended Classification of Pesticides by Hazard and guidelines to classification, 2019 edition (2020), https://www.who.int/publications/i/item/9789240005662, Accessed 23 May 2025.
• F. Eissa, S. El-Sawi, N. E. Zidan, Determining pesticide residues in honey and their potential risk to consumers, Pol. J. Environ. Stud. 23 (5) (2014) 1573–1580.
• M. Saitta, G. Di Bella, M. R. Fede, V. Lo Turco, A. G. Potortì, R. Rando, M. T. Russo, G. Dugo, Gas chromatography-tandem mass spectrometry multi-residual analysis of contaminants in Italian honey samples, Food Addit Contam Part A Chem Anal Control Expo Risk Assess 34 (5) (2017) 800–808.
• F. Kara, C. Kaya, E. E. Yücel, S. Topuz, M. Bayram, Determination of some physicochemical properties of honeys from Tokat region and their compliance with the Turkish food codex, Uludag Bee Journal 22 (2) (2022) 148–165.
• I. Simsek, O. Kuzukiran, B. Yurdakok-Dikmen, U. T. Sireli, M. Beykaya, A. Filazi, Comparison of selected lipophilic compound residues in honey and propolis, Journal of Food Composition and Analysis 102 (2021) 104068.
• A. M. K. Nassar, Y. M. Salim, E. Nour-Eldeen, M. S. Younis, M. M. Kelany, M. A. Shebl, A. S. Shafey, H. F. Abou-Sharara, Seasonal screening of pesticide residues in beehive products collected from different districts in Egypt, Environ Monit Assess 196 (2024) 297.
• H. Seçimiş Canbay, S. Öğüt, M. Yılmazer, E. Küçüköner, Analysis of selected some pesticides in honey samples, Süleyman Demirel University Journal of Natural and Applied Sciences 16 (1) (2012) 1–5.
• İ. Toptancı, A. Bayrak, Determination of pesticide residue levels in citrus honeys, Academic Food Journal 10 (3) (2012) 22–25.
• Y. Şahin, V. Armağan, A. Yılmaz Aslantürk, Missing link in environmental policy: Honey bees, Contemporary Local Governments 32 (2) (2023) 31–54.
• C. Muku, G. Güçlü, S. Selli, Determination of pesticide and naphthalene residues in eastern Mediterranean honey by LC/MS/MS and HS-SPME GC/MS, Çukurova Journal of Agriculture and Food Sciences 34 (2) (2019) 142–148.
• İ. Polat, The investıgatıon of the antimicrobial, antioxidant activities and the contamination of pesticide and antibiotic residues of some honey produced in South Marmara region, Master’s Thesis Balıkesir University (2011) Balıkesir.
• E. Çakar, F. Gürel, Comparison of liquid and comb honeys for pesticide, naphthalene and antibiotic residues, Mediterranean Agricultural Sciences 32 (3) (2019) 453–459.
• M. A. Kutlu, A. Ş. Bengü, Determination of antibiotic and pesticide residues and some quality criteria in honey produced in Muş province, BÜSAD 1 (1) (2020) 1–6.
• N. Ozdemir, M.N. Muz, Neonicotinoid Analysis in Sunflower (Helianthus annuus) Honey samples collected around Tekirdag in Turkey, International Journal of Analytical Chemistry 2023 (1) (2023) 429449.
• S. A. Ashraf, D. Mahmood, A. E. O. Elkhalifa, A. J. Siddiqui, M. I. Khan, F. Ashfaq, M. Patel, M. Snoussi, M. Kieliszek, M. Adnan, Exposure to pesticide residues in honey and its potential cancer risk assessment, Food Chem Toxicol 180 (2023) 114014.