THE CHEMICAL COMPOSITION OF CENTAUREA FURFURACEA COSS. AND DUR. ESSENTIAL OIL WITH ANTIOXIDANT, ANTICHOLINESTERASE AND ANTIBIOFILM ACTIVITIES

Abstract

The aim of this work was to conduct the chemical composition of essential oil EO of Centaureafurfuraceafrom Algerian Sahara and investigate the antioxidant, anticholinesterase, antimicrobial andantibiofilm activities of its essential oil and methanol extract. EO of C. furfuracea was analyzed byGC and GC-MS. Sixty-nine compounds identified, representing 96.94 % of the totaloil. Caryophyllene oxide 12.01 % , Z-10-pentadecen-1-ol 8.11 % , farnesyl methylester 7.79 % were identified as the main constituents. The antioxidant activity was determined using threecomplementary assays, in lipid peroxidation inhibition the methanol extract showed the best activitywith an IC50 = 28.73 ± 0.29 µg/mL followed by EO with an IC50 = 95.95 ± 15.20 µg/mL. Whilein DPPH and CUPRAC assays, EO and methanol extract indicated a less to moderate activity. The invitro anticholinesterase activity, the methanol extract showed moderate inhibitory activities againstacetylcholinesterase IC50= 164.4 ± 5.69 µg/mL and butyrylcholinesterase IC50 = 82.4±1.75 µg/mL ,while EO was inactive against both enzymes. Minimum inhibitory concentrations MICs werecalculated by microtitre broth dilution method, and antibiofilm effect by microplate biofilm assay.EO inhibited the growth of all tested microorganisms, MIC values were between 6.25 and 25 µL/mLconcentrations, better than methanol extract. The highest antibiofilm activity have reached to 87.90 %with methanol extract of C. furfuracea against Staphylococcus aureus ATCC 6538 Pat 50 mg/mLconcentration.These results showed that C. furfuracea is a natural source of active compounds withantibiotic and antibiofilm effects against S. aureusand B. subtilis, and Bacillus cereus, respectively,and also presents antioxidant and anticholinesterase properties

Keywords

• Antioxidant,Antibiofilm,Anticholinesterase,Centaurea Furfuracea,Essential Oil

References

1. Adams, Robert P (2007). Identification of essential oil components by gas chromatography/mass spectrometry