ANTIMICROBIAL AND UREASE INHIBITION ACTIVITY OF VOLATILE OIL OBTAINED FROM AERIAL PARTS OF THYME THYMUS VULGARIS L.

Abstract

The demand for T. vulgaris L. Thyme and its derivates has became popular since it has manybiologically active components such as mainly antimicrobial, antiinflammatory, antioxidantcharacteristics. Especially, there were many researches on the antimicrobial performance of T.vulgaris L. essential oil. In this study, we analyzed the content of the essential oil of T. vulgaris aerialparts by using GCMS, its antimicrobial activity to some pathogens and urease inhibition activity. Themajor components of the oil in thyme were thymol 48.80% , γ-terpinene 15.26% , p-cymene 10.35% , linalol 3.87% , β-myrcene 3.20% . Then, The minimum inhibitory concentration MIC were determined against Escherichia coli ATCC 25293 , Bacillus subtilis ATCC 6633 ,Staphylococcus aureus ATCC 25925 , Pseudomonas aureginosa, Candida albicans and Candidaparapsilosis by using spectrophotometric microbroth technique. We showed that microoganismswere inhibited by the essential oil extracted thyme aerial parts at the end of the 24 hours incubation.Highest inhibition were reported against S. aureus MIC=7.5 µg/mL , while lowest inhibition werefound to be C. albicans MIC=105.3 µg/mL . Also, the antiurease activities were tested againsturease enzymes such as thiourea, spectrophotometrically. The essential oil 27.40±0.46% of thymeshowed higher urease inhibition activity than thiourea 23.08±0.19% . Consequently, it clearlysuggested that the essential oil from T. vulgaris aerial parts are a potential source of antimicrobialand urease inhibitory ingredients for the drug and food industry

Keywords

• Chemical composition,Antimicrobial activity,Thymus vulgaris,Urease inhibition activity,Volatile oil

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