BibTex RIS Cite

The role of serum matrix metalloproteinase-9 levels in the etiopathogenesis of nasal polyps

Year 2012, Volume: 2 Issue: 2, 53 - 57, 01.06.2012

Mustafa Acar Özer Erdem Gür Mustafa Kaymakçı Onur Çiftçi Güler Buğdaycı Cafer Özdem

https://doi.org/10.2399/jmu.2012002002

Abstract

Objective: The aim of this study is to reveal the role of matrix metalloproteinase-9 in the pathogenesis of nasal polyps by comparing its levels in normal healthy subjects, and patients with nasal polyps. Our second aim is to demonstrate the correlation (if any) between white blood cell counts, age, gender of the patients presence of asthma, and MMP-9 levels. Methods: Serum samples of patients were obtained and kept at -70°C. Quantikine kit (R&D System Inc, Minneapolis, MN, USA) micro-ELISA (Enzyme-Linked Immunosorbent Assay) method was used to estimate plasma matrix metalloproteinase-9 levels. Serum matrix metalloproteinase-9 values of the control, and the patient groups were compared. The correlation between age, gender of the study subjects, white blood cell counts and the presence of asthma was investigated. Results: Serum matrix metalloproteinase-9 values were significantly higher in patients with nasal polyps relative to the control group. White blood cell counts in the group with nasal polyps were higher without any statistically significant difference between the groups. Any correlation between matrix metalloproteinase-9 values, age, and gender was not found. In addition, any correlation was not detected between concomitant asthmatic disease and matrix metalloproteinase-9 values. Conclusion: Significantly, higher matrix metalloproteinase-9 values in patients with nasal polyps support our opinion suggesting the role of this protein in the etiopathogenesis of this disease.

Keywords

Nasal polyp matrix metalloproteinase-9 etiopathogenesis

References

  • Van Cauwenberge P, Van Zele T, Bachert C. Chronic rhinonsi- nusitis and nasal polyposis: the etiopathogenesis revealed? Verh K Acad Geneeskd Belg 2008;70:305-22.
  • Pastorello EA, Incorvaia C, Riario-Sforza GG, Codecasa L, Menghisi V, Bianchi C. Importance of allergic etiology in nasal polyposis. Allergy Proc 1994;15:151-5.
  • Mudd PA, Katial RK, Alam R, Hohensee S, Ramakrishnan V, Kingdom TT. Variations in expression of matrix metallopro- teinase-9 and tissue inhibitor of metalloproteinase-1 in nasal mucosa of aspirin-sensitive versus aspirin-tolerant patients with nasal polyposis. Ann Allergy Asthma Immunol 2011;107:353-9.
  • Wang LF, Chien CY, Tai CF, Kuo WR, Hsi E, Juo SH. Matrix metalloproteinase-9 gene polymorphisms in nasal polyposis. BMC Med Genet 2010; 11:85.
  • Erbek SS, Yurtcu E, Erbek S, Sahin FI. Matrix metallopro- teinase-9 promoter gene polymorphism (-1562C>T) in nasal polyposis. Am J Rhinol Allergy 2009;23:568-70.
  • Wang LF, Chien CY, Chiang FY, Chai CY, Tai CF. Corelationship between matrix metalloproteinase 2 and 9 expression and severity of chronic rhinosinusitis with nasal poly- posis. Am J Rhinol Allergy 2012;26(1):e1-4.
  • Kennedy DW. Prognostic factors, outcomes and staging in eth- moid sinus surgery. Laryngoscope 1992;102(12 Pt 2 Suppl 57):1- 18.
  • Krane SM. Clinical importance of metalloproteinases and their inhibitors. Ann N Y Acad Sci 1994;732:1-10.
  • Watelet JB, Demetter P, Claeys C, Van Cauwenberge P, Cuvelier C, Bachert C. Neutrophil-derived metalloproteinase-9 predicts healing quality after sinus surgery. Laryngoscope 2005;115:56-61.
  • Bhandari A, Takeuchi K, Suzuki S, et al. Increased expression of matrix metalloproteinase-2 in nasal polyps. Acta Otolaryngol 2004; 124:1165-70.
  • Watelet JB, Claeys C, Van Cauwenberge P, et al. Predictive and monitoring value of matrix metalloproteinase-9 for healing qual- ity after sinus surgery. Wound Repair Regen 2004;12:412-8.
  • Vignola AM, Riccobono L, Mirabella A, et al. Sputum metallo- proteinase-9/tissue inhibitor of metalloproteinase-1 ratio corre- lates with airflow obstruction in asthma and chronic bronchitis. Am J Respir Crit Care Med 1998;158:1945-50.
  • Kumagai K, Ohno I, Okada S, et al. Inhibition of matrix metal- loproteinases prevents allergen-induced airway inflammation in a murine model of asthma. J Immunol 1999;162:4212-9.
  • Lemjabbar H, Gosset P, Lamblin C, et al. Contribution of 92 kDa gelatinase/type IV collagenase in bronchial inflammation during status asthmaticus. Am J Respir Crit Care Med 1999; 159(4 Pt 1):1298-307.
  • Selman M, Ruiz V, Cabrera S, et al. TIMP-1, -2, -3, and -4 in idiopathic pulmonary fibrosis. A prevailing nondegradative lung microenvironment? Am J Physiol Lung Cell Mol Physiol 2000; 279:L562-74.
  • Lechapt-Zalcman E, Coste A, d'Ortho MP, et al. Increased expression of matrix metalloproteinase-9 in nasal polyps. J Pathol 2001;193:233-41.
  • Lee YM, Kim SS, Kim HA, et al. Eosinophil inflammation of nasal polyp tissue: relationships with matrix metalloproteinases, tissue inhibitor of metalloproteinase-1, and transforming growth factor-beta1. J Korean Med Sci 2003;18:97-102.
  • Bu açık erişim makalenin, ticari kullanım amacı ve içerik değişikliği dışında kalan çoğaltma, dağıtma vb. tüm kullanım hakları, bilinen standartlarda kay
  • nak olarak gösterilmesi koşuluyla Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported (CC BY-NC-ND3.0) Lisansı aracılığıyla
  • (http://creativecommons.org/licenses/by-nc-nd/3.0/) bedelsiz kullanıma sunulmuştur.
  • Makalenin atıf künyesi: Acar M, Gür ÖE, Kaymakçı M, Çiftçi O, Buğdaycı G, Özdem C. Nazal polipli hastalarda serum matriks metalloproteinaz-9
  • seviyelerinin hastalığın etiyopatogenezindeki rolü. J Med Updates 2012;2(2):53-57.

Nazal polipli hastalarda serum matriks metalloproteinaz-9 seviyelerinin hastalığın etiyopatogenezindeki rolü

Year 2012, Volume: 2 Issue: 2, 53 - 57, 01.06.2012

Mustafa Acar Özer Erdem Gür Mustafa Kaymakçı Onur Çiftçi Güler Buğdaycı Cafer Özdem

https://doi.org/10.2399/jmu.2012002002

Abstract

Amaç: Bu çalışmanın amacı, nazal polipte serum matriks metalloproteinaz-9 düzeyini normal hastalarla karşılaştırmak suretiyle, nazal polip oluşumunda matriks metalloproteinaz-9'un rolünü ortaya koymaktır. İkincil amacımız ise, hastaların lökosit değerleri, yaşları, cinsiyeti ve astım varlığı ile matriks metalloproteinaz 9 arasında herhangi bir ilişki olup olmadığını göstermektir. Yöntem: Hastaların serum örnekleri alınıp -70°C sıcaklıkta saklandı. Plazmada matriks metalloproteinaz-9 seviyesini saptamak için Quantikine kit (R&D System Inc, Minneapolis, MN, ABD) mikro-elisa (Enzyme-Linked Immunosorbent Assay) yöntemi kullanıldı. Hastaların serum örneklerinde nazal polipli grupla kontrol grubu arasında serumdaki matriks metalloproteinaz-9 değerleri karşılaştırıldı. Yaş, cinsiyet, lökosit sayısı ve astım varlığının matriks metalloproteinaz-9 ile ilişkisi incelendi. Bulgular: Nazal polipli hastalarda serum matriks metalloproteinaz-9 değerleri kontrol grubuna göre anlamlı derecede yüksekti. Lökosit sayıları karşılaştırıldığında nazal polipli grupta daha yüksek değerler mevcut iken istatistiksel olarak iki grup arasında anlamlı bir fark bulunmadı. Hastaların matriks metalloproteinaz-9 değerlerinin yaşla ve cinsiyetle herhangi bir ilişkisi bulunamadı. Astım birlikteliği ile matriks metalloproteinaz-9 değerleri arasında herhangi bir korelasyon bulunamadı. Sonuç: Nazal polipli hastalarda matriks metalloproteinaz-9 değerinin anlamlı derecede yüksek bulunması, bu proteinin hastalığın etiyopatogenezinde rolü olduğu yönündeki düşüncemizi desteklemektedir.

Keywords

Nazal polip matriks metalloproteinaz-9 etiyopatogenez

References

  • Van Cauwenberge P, Van Zele T, Bachert C. Chronic rhinonsi- nusitis and nasal polyposis: the etiopathogenesis revealed? Verh K Acad Geneeskd Belg 2008;70:305-22.
  • Pastorello EA, Incorvaia C, Riario-Sforza GG, Codecasa L, Menghisi V, Bianchi C. Importance of allergic etiology in nasal polyposis. Allergy Proc 1994;15:151-5.
  • Mudd PA, Katial RK, Alam R, Hohensee S, Ramakrishnan V, Kingdom TT. Variations in expression of matrix metallopro- teinase-9 and tissue inhibitor of metalloproteinase-1 in nasal mucosa of aspirin-sensitive versus aspirin-tolerant patients with nasal polyposis. Ann Allergy Asthma Immunol 2011;107:353-9.
  • Wang LF, Chien CY, Tai CF, Kuo WR, Hsi E, Juo SH. Matrix metalloproteinase-9 gene polymorphisms in nasal polyposis. BMC Med Genet 2010; 11:85.
  • Erbek SS, Yurtcu E, Erbek S, Sahin FI. Matrix metallopro- teinase-9 promoter gene polymorphism (-1562C>T) in nasal polyposis. Am J Rhinol Allergy 2009;23:568-70.
  • Wang LF, Chien CY, Chiang FY, Chai CY, Tai CF. Corelationship between matrix metalloproteinase 2 and 9 expression and severity of chronic rhinosinusitis with nasal poly- posis. Am J Rhinol Allergy 2012;26(1):e1-4.
  • Kennedy DW. Prognostic factors, outcomes and staging in eth- moid sinus surgery. Laryngoscope 1992;102(12 Pt 2 Suppl 57):1- 18.
  • Krane SM. Clinical importance of metalloproteinases and their inhibitors. Ann N Y Acad Sci 1994;732:1-10.
  • Watelet JB, Demetter P, Claeys C, Van Cauwenberge P, Cuvelier C, Bachert C. Neutrophil-derived metalloproteinase-9 predicts healing quality after sinus surgery. Laryngoscope 2005;115:56-61.
  • Bhandari A, Takeuchi K, Suzuki S, et al. Increased expression of matrix metalloproteinase-2 in nasal polyps. Acta Otolaryngol 2004; 124:1165-70.
  • Watelet JB, Claeys C, Van Cauwenberge P, et al. Predictive and monitoring value of matrix metalloproteinase-9 for healing qual- ity after sinus surgery. Wound Repair Regen 2004;12:412-8.
  • Vignola AM, Riccobono L, Mirabella A, et al. Sputum metallo- proteinase-9/tissue inhibitor of metalloproteinase-1 ratio corre- lates with airflow obstruction in asthma and chronic bronchitis. Am J Respir Crit Care Med 1998;158:1945-50.
  • Kumagai K, Ohno I, Okada S, et al. Inhibition of matrix metal- loproteinases prevents allergen-induced airway inflammation in a murine model of asthma. J Immunol 1999;162:4212-9.
  • Lemjabbar H, Gosset P, Lamblin C, et al. Contribution of 92 kDa gelatinase/type IV collagenase in bronchial inflammation during status asthmaticus. Am J Respir Crit Care Med 1999; 159(4 Pt 1):1298-307.
  • Selman M, Ruiz V, Cabrera S, et al. TIMP-1, -2, -3, and -4 in idiopathic pulmonary fibrosis. A prevailing nondegradative lung microenvironment? Am J Physiol Lung Cell Mol Physiol 2000; 279:L562-74.
  • Lechapt-Zalcman E, Coste A, d'Ortho MP, et al. Increased expression of matrix metalloproteinase-9 in nasal polyps. J Pathol 2001;193:233-41.
  • Lee YM, Kim SS, Kim HA, et al. Eosinophil inflammation of nasal polyp tissue: relationships with matrix metalloproteinases, tissue inhibitor of metalloproteinase-1, and transforming growth factor-beta1. J Korean Med Sci 2003;18:97-102.
  • Bu açık erişim makalenin, ticari kullanım amacı ve içerik değişikliği dışında kalan çoğaltma, dağıtma vb. tüm kullanım hakları, bilinen standartlarda kay
  • nak olarak gösterilmesi koşuluyla Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported (CC BY-NC-ND3.0) Lisansı aracılığıyla
  • (http://creativecommons.org/licenses/by-nc-nd/3.0/) bedelsiz kullanıma sunulmuştur.
  • Makalenin atıf künyesi: Acar M, Gür ÖE, Kaymakçı M, Çiftçi O, Buğdaycı G, Özdem C. Nazal polipli hastalarda serum matriks metalloproteinaz-9
  • seviyelerinin hastalığın etiyopatogenezindeki rolü. J Med Updates 2012;2(2):53-57.
There are 22 citations in total.

Details

Primary Language Turkish
Journal Section Articles
Authors

Mustafa Acar This is me

Özer Erdem Gür This is me

Mustafa Kaymakçı This is me

Onur Çiftçi This is me

Güler Buğdaycı This is me

Cafer Özdem This is me

Publication Date June 1, 2012
Published in Issue Year 2012 Volume: 2 Issue: 2

Cite

APA Acar, M., Gür, Ö. E., Kaymakçı, M., Çiftçi, O., et al. (2012). Nazal polipli hastalarda serum matriks metalloproteinaz-9 seviyelerinin hastalığın etiyopatogenezindeki rolü. Journal of Medical Updates, 2(2), 53-57. https://doi.org/10.2399/jmu.2012002002
AMA Acar M, Gür ÖE, Kaymakçı M, Çiftçi O, Buğdaycı G, Özdem C. Nazal polipli hastalarda serum matriks metalloproteinaz-9 seviyelerinin hastalığın etiyopatogenezindeki rolü. Journal of Medical Updates. June 2012;2(2):53-57. doi:10.2399/jmu.2012002002
Chicago Acar, Mustafa, Özer Erdem Gür, Mustafa Kaymakçı, Onur Çiftçi, Güler Buğdaycı, and Cafer Özdem. “Nazal Polipli Hastalarda Serum Matriks Metalloproteinaz-9 Seviyelerinin hastalığın Etiyopatogenezindeki Rolü”. Journal of Medical Updates 2, no. 2 (June 2012): 53-57. https://doi.org/10.2399/jmu.2012002002.
EndNote Acar M, Gür ÖE, Kaymakçı M, Çiftçi O, Buğdaycı G, Özdem C (June 1, 2012) Nazal polipli hastalarda serum matriks metalloproteinaz-9 seviyelerinin hastalığın etiyopatogenezindeki rolü. Journal of Medical Updates 2 2 53–57.
IEEE M. Acar, Ö. E. Gür, M. Kaymakçı, O. Çiftçi, G. Buğdaycı, and C. Özdem, “Nazal polipli hastalarda serum matriks metalloproteinaz-9 seviyelerinin hastalığın etiyopatogenezindeki rolü”, Journal of Medical Updates, vol. 2, no. 2, pp. 53–57, 2012, doi: 10.2399/jmu.2012002002.
ISNAD Acar, Mustafa et al. “Nazal Polipli Hastalarda Serum Matriks Metalloproteinaz-9 Seviyelerinin hastalığın Etiyopatogenezindeki Rolü”. Journal of Medical Updates 2/2 (June 2012), 53-57. https://doi.org/10.2399/jmu.2012002002.
JAMA Acar M, Gür ÖE, Kaymakçı M, Çiftçi O, Buğdaycı G, Özdem C. Nazal polipli hastalarda serum matriks metalloproteinaz-9 seviyelerinin hastalığın etiyopatogenezindeki rolü. Journal of Medical Updates. 2012;2:53–57.
MLA Acar, Mustafa et al. “Nazal Polipli Hastalarda Serum Matriks Metalloproteinaz-9 Seviyelerinin hastalığın Etiyopatogenezindeki Rolü”. Journal of Medical Updates, vol. 2, no. 2, 2012, pp. 53-57, doi:10.2399/jmu.2012002002.
Vancouver Acar M, Gür ÖE, Kaymakçı M, Çiftçi O, Buğdaycı G, Özdem C. Nazal polipli hastalarda serum matriks metalloproteinaz-9 seviyelerinin hastalığın etiyopatogenezindeki rolü. Journal of Medical Updates. 2012;2(2):53-7.