Diyabetik Sıçan Modelinde Sarı Kantaron Yağı ve Tiyosilin Merheminin Yara İyileşmesi Üzerine Karşılaştırılması

Abstract

Amaç: Diyabetik hastalarda yara iyileşmesi bozulur ve yaralar sıklıkla multibakteriyel ajanlarla enfekte olur. Bu çalışma, diyabetik enfekte yara modelinde yara iyileşmesini iyileştirmede St. John's wort ve basitrasin-neomisin (tiyosillin) içeren merhemin etkinliğini karşılaştırmayı amaçladı. Yöntemler: 60 mg/kg streptozotosin (STZ) uygulanarak diyabet oluşturulan sıçanlar, 72 saat sonra kan şekeri düzeyleri 300 mg/dl'nin üzerine çıktığında diyabet hastası olarak kabul edildi. Grup 1: Kontrol (Diyabetik olmayan) grubu, Grup 2: Diyabet grubu. Yara bakımı sırasında her iki grup da PI ile dezenfekte edildi ve her sıçanın sağ lomber bölgesine tiyosillin, sol lomber bölgesine ise sarı kantaron yağı uygulandı. Yara iyileşme süresi dikkate alınarak ortalama 20 gün sonra çalışma sonlandırıldı. Histopatolojik incelemede ülserasyon, nekroz, epitelizasyon, konjesyon, ödem, polimorf çekirdekli lökosit (PNL), monosit, fibroblast ve neovaskülarizasyon değerlendirildi. Bulgular: Histopatolojik değerlendirmede, sarı kantaron yağı uygulanan grupta tiyosilin verilen gruba göre ülserasyon ve nekrozda istatistiksel olarak anlamlı azalma görüldü (p=0,04). Epitelizasyon açısından, sarı kantaron yağı uygulanan grupta tiyosilin verilen gruba göre istatistiksel olarak anlamlı bir artış görüldü (p=0,03). St. John's wort yağı ile tedavi edilen grupta, tiyosilin verilen gruba göre konjesyon ve ödemde istatistiksel olarak anlamlı azalma görüldü (p=0,03). St. John's Wort yağı uygulanan grupta tiyosilin verilen gruba göre Fibroblast ve Neovaskülarizasyonda istatistiksel olarak anlamlı artış görüldü (p=0,02). Sonuçlar: Histopatolojik olarak yara iyileşme sürecinde önemli işlevleri olan epitelizasyon, fibroblast ve neovaskülarizasyon, St. John's wort uygulanan diyabetik sıçanlarda arttı. Geleneksel tıpta antidepresan etkinliği nedeniyle kullanılmasına rağmen yara iyileşme sürecini artırma potansiyeline sahip olması nedeniyle sarı kantaron bitkisinin diyabet hastalarında gelişen yaralarda kullanılabileceğini düşünüyoruz.

Keywords

• Diyabet
• sıçan
• yara modeli
• Sarı Kantaron Yağı
• tiyosilin
• yara iyileşmesi

Comparison of St. John's wort oil and thiocilline ointment on wound healing in a diabetic rat model

Abstract

Aims: In diabetic patients, wound healing is impaired and wounds are often infected with multifactorial agents. This study aimed to compare the effectiveness of St. John’s wort and ointment containing bacitracin-neomycin (thiocilline) to improve wound healing in a diabetic infected wound model. Methods: Rats in which diabetes was induced by administering 60 mg/kg streptozotocin (STZ) were considered diabetic if their blood sugar levels were above 300 mg/dl 72 hours later. Group 1: Control (Non diabetic) group, Group 2: Diabetes group. During wound care, both groups were disinfected with povidone iodine (PI) and the right lumbar region of each rat was dressed with thiocilline and the left lumbar region was dressed with St. John’s wort oil. Considering the wound healing period, the study was terminated after an average of 20 days. In histopathological examination, ulceration, necrosis, epithelialization, congestion, edema, polymorphous nucleated leukocyte (PNL), monocyte, fibroblast, and neovascularization were evaluated. Results: In histopathological evaluation, there was a statistically significant decrease in ulceration and necrosis in the group treated with St. John’s wort oil compared to the group given thiocilline (p=0.04). In terms of epithelialization, there was a statistically significant increase in the group dressed with St. John’s wort oil compared to the group given thiocilline (p=0.03). There was a statistically significant decrease in congestion and edema in the group treated with St. John’s wort oil compared to the group given thiocilline (p=0.03). There was a statistically significant increase in fibroblast and neovascularization in the group treated with St. John’s wort oil compared to the group given thiocilline (p=0.02). Conclusion: Histopathologic ally, epithelialization, fibroblast, and neovascularization, which have important functions in the wound healing process, increased in diabetic rats administered St. John’s wort. Although it is used in traditional medicine due to its antidepressant effectiveness, we believe that St. John’s wort can be used in wounds that develop in diabetic patients, as it has the potential to increase the wound healing process.

Keywords

• Diabetes
• rat
• wound model
• St. John
• thiocilline
• wound healing

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