Fast-disintegrating under-eye mask: postbiotic-loaded polyvinylpyrrolidone nanofibers for anti-aging and hydration application

Year 2026, Volume: 7 Issue: 2, 391 - 400, 27.03.2026

Muhammet Sait Toprak , Ece Guler , Hümeyra Betül Yekeler , Gülsüm Ercan , Deniz Baybağ , Aleyna Çavdar , Nedim Can Çevik Muhammet Emin Çam

https://izlik.org/JA57LB44ZM

Abstract

Aims: The aim of this study was to develop and characterize postbiotic-loaded polyvinylpyrrolidone (PVP) nanofibers (PBNF) as a fast-disintegrating under-eye delivery system and to investigate their in vitro performance, including physicochemical properties, release kinetics, biocompatibility, and their potential to modulate skin-related cellular and molecular responses.
Methods: PBNFs containing 0.5%, 1%, and 1.5% postbiotics were fabricated using pressurized gyration (PG) technology. Pure nanofibers (PNF) served as controls. The nanofibers were characterized for morphology (SEM), chemical structure (FTIR), encapsulation efficiency, dissolution behavior, release kinetics, and disintegration properties. In vitro biological performance was assessed using L929 fibroblast and HaCaT keratinocyte cells through WST-1 viability assays and morphological analysis. Additionally, gene expression analysis was performed by qPCR to evaluate the molecular effects of the formulations on extracellular matrix remodeling and inflammatory markers.
Results: SEM analysis revealed uniform, bead-free nanofibers with comparable diameters across all formulations, indicating that postbiotic loading did not alter fiber morphology. FTIR spectra confirmed the successful incorporation of postbiotics into the PVP matrix. Among the formulations, 0.5% PBNF exhibited the highest encapsulation efficiency and was selected for further evaluation. Dissolution studies demonstrated a rapid initial release followed by diffusion-controlled kinetics best described by the Higuchi model. Disintegration tests showed ultrafast wetting and dissolution of the nanofibers in PBS within seconds. In vitro studies demonstrated that PBNFs promoted cell viability and proliferation in a dose- and time-dependent manner, resulting in a more balanced and controlled biological response compared to free postbiotics or PNF, without inducing cytotoxic effects. qPCR analysis demonstrated a coordinated modulation of extracellular matrix-related genes, characterized by decreased MMP1 and increased COL1A1 expression in PBNF groups, while IL6 levels remained stable.
Conclusion: PBNF produced by PG represents a biocompatible, fast-disintegrating, and effective topical delivery system with strong potential for use in under-eye cosmetics and regenerative applications.

Keywords

Postbiotic , nanofiber , pressurized gyration , anti-aging , hydration , under-eye mask

Ethical Statement

There is no Ethical Statement in this study.

Supporting Institution

There is no Supporting Institution in this study.

Project Number

-

Thanks

We would like to thank the owner of Shymeriae Biotechnology, Leyla Tarhan Celebi, for providing postbiotics to our study.

References

• Jeng L, Mirchandani A. Chapter 20-Skin health: what damages and ages skin? Evidence-based interventions to maintain healthy skin. In: Short E, ed. A Prescription for Healthy Living. Academic Press; 2021:225-233. doi:10.1016/B978-0-12-821573-9.00020-5
• Park K. Role of micronutrients in skin health and function. Biomol. Ther. (Seoul). 2015;23(3):207-217. doi:10.4062/biomolther.2015.003
• Rostami F, Yekrang J, Gholamshahbazi N, Ramyar M, Dehghanniri P. Under-eye patch based on PVA-gelatin nanocomposite nanofiber as a potential skin care product for fast delivery of the coenzyme Q10 anti-aging agent: in vitro and in vivo studies. Emerg. Mater. 2023;6(6):1903-1921. doi:10.1007/s42247-023-00587-9
• Bellu E, Garroni G, Cruciani S, et al. Smart nanofibers with natural extracts prevent senescence patterning in a dynamic cell culture model of human skin. Cells. 2020;9(12):2530. doi:10.3390/cells9122530
• Yiğit İK, Türsen Ü, Türsen B, Solak B, Bakay ÖSK, Kroumpouzos G. Probiotics for skin aging and skin conditions in the elderly. Clin. Dermatol. 2026. doi:10.1016/j.clindermatol.2026.02.016
• Cardoso AJR, Carvalho SG, Mantovanelli VR, et al. Postbiotics: modulation of the gut microbiota and potential for association with nanotechnology. Probiotics Antimicrob Proteins. 2025:1-19. doi:10.1007/s12602-025-10675-3
• Liu H, Bai Y, Huang C, et al. Recent progress of electrospun herbal medicine nanofibers. Biomol. 2023;13(1):184. doi:10.3390/biom13010184
• Kurakula M, Rao GK. Pharmaceutical assessment of polyvinylpyrrolidone (PVP): as excipient from conventional to controlled delivery systems with a spotlight on COVID-19 inhibition. J Drug Deliv Sci Technol. 2020;60: 102046. doi:10.1016/j.jddst.2020.102046
• Doustdar F, Ghorbani M. ZIF-8 enriched electrospun ethyl cellulose/polyvinylpyrrolidone scaffolds: the key role of polyvinylpyrrolidone molecular weight. Carbohydr Polym. 2022;291:119620. doi:10.1016/j.carbpol.2022.119620
• Aguilar-Toalá J, Garcia-Varela R, Garcia H, et al. Postbiotics: an evolving term within the functional foods field. Trends Food Sci Technol. 2018;75: 105-114. doi:10.1016/j.tifs.2018.03.009
• Zendeboodi F, Khorshidian N, Mortazavian AM, da Cruz AG. Probiotic: conceptualization from a new approach. Curr Opin Food Sci. 2020;32: 103-123. doi:10.1016/j.cofs.2020.03.009
• Salminen S, Collado MC, Endo A, et al. The International Scientific Association of Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of postbiotics. Nat Rev Gastroenterol Hepatol. 2021;18(9):649-667. doi:10.1038/s41575-021-00440-6
• Nataraj BH, Ali SA, Behare PV, Yadav H. Postbiotics-parabiotics: the new horizons in microbial biotherapy and functional foods. Microb Cell Fact. 2020;19(1):168. doi:10.1186/s12934-020-01426-w
• Cam ME, Ertas B, Alenezi H, et al. Accelerated diabetic wound healing by topical application of combination oral antidiabetic agents-loaded nanofibrous scaffolds: an in vitro and in vivo evaluation study. Mater Sci Eng C. 2021;119:111586. doi:10.1016/j.msec.2020.111586
• Yekeler HB, Kabaoglu I, Guler E, et al. A comparison of electrospinning and pressurized gyration: production of empagliflozin-loaded polylactic acid/polycaprolactone fibrous patches. J R Soc Interface. 2025;22(224): 20240635. doi:10.1098/rsif.2024.0635
• Ácsová A, Hojerová J, Martiniaková S. Efficacy of postbiotics against free radicals and UV radiation. Chem Pap. 2022;76(4):2357-2364. doi:10. 1007/s11696-021-02018-7
• Maleki M, Mehrabi M, Mehrabi M, Malvajerd SS. Comparative kinetic modeling and mathematical analysis of monoterpene release from nanocarrier systems. AAPS PharmSciTech. 2026;27(3):118. doi:10.1208/s12249-026-03332-7
• Naig PJE, Kuo Z-Y, Wu Y-S, Hung K-Y. Electrospun carbon black-incorporated nanofibers for enhanced facial sebum adsorption. IEEE. 2025:104-108. doi:10.1109/NANOMED68094.2025.11431377
• Guler E, Yekeler HB, Abobakr FKM, et al. In vitro and in vivo evaluation of rectal delivery of novel sulfasalazine-loaded hydrogels and nanofibers for enhanced ulcerative colitis therapy. J Drug Deliv Sci Technol. 2025: 107960. doi:10.1016/j.jddst.2025.107960
• Guan H, Yang K. RNA isolation and real-time quantitative RT-PCR. Methods Mol Biol. 2008;456:259-270. doi:10.1007/978-1-59745-245-8_19
• Guler E, Yekeler HB, Parviz G, et al. Vitamin B12-loaded chitosan-based nanoparticle-embedded polymeric nanofibers for sublingual and transdermal applications: two alternative application routes for vitamin B12. Int J Biol Macromol. 2024;258:128635. doi:10.1016/j.ijbiomac.2023. 128635
• Krithika R, Balasasirekha R. FTIR spectrum and XRD of postbiotics-exopolysaccharides zinc oxide nanoparticles. J Adv Sci Res. 2021;12(03 Suppl 2):292-300. doi:10.55218/JASR.s2202112334
• Siepmann J, Peppas NA. Higuchi equation: derivation, applications, use and misuse. Int J Pharm. 2011;418(1):6-12. doi:10.1016/j.ijpharm.2011. 03.051
• Veysanoglu S, Ertas B, Guler E, et al. In vitro and in vivo evaluation of multi-target-directed Rivastigmine/Memantine/Gingko biloba-loaded nanofibers against Alzheimer's disease. J Drug Deliv Sci Technol. 2023; 86:104691. doi:10.1016/j.jddst.2023.104691
• Kiňová Sepová H, Florová B, Bilková A, Drobná E, Březina V. Evaluation of adhesion properties of lactobacilli probiotic candidates. Monatsh Chem. 2018;149(5):893-899. doi:10.1007/s00706-017-2135-1
• Cesur NP, Zad Ghaffari Vahdat K, Türkoğlu Laçin N. Fabrication of bacterial cellulose/PVP nanofiber composites by electrospinning. Biopolymers. 2024;115(5):e23606. doi:10.1002/bip.23606
• Machado P, Ribeiro FN, Giublin FC, et al. Next-generation wound care: a scoping review on probiotic, prebiotic, synbiotic, and postbiotic cutaneous formulations. Pharmaceuticals. 2025;18(5):704. doi:10.3390/ph18050704
• Lee JY, Park JY, Jeong Y, Kang CH. Anti-inflammatory response in TNFα/IFNγ-induced HaCaT keratinocytes and probiotic properties of Lacticaseibacillus rhamnosus MG4644, Lacticaseibacillus paracasei MG4693, and Lactococcus lactis MG5474. J Microbiol Biotechnol. 2023; 33(8):1039-1049. doi:10.4014/jmb.2301.01028
• Park J-Y, Lee JY, Kim Y, Kang C-H. Lactic acid bacteria improve the photoprotective effect via MAPK/AP-1/MMP signaling pathway on skin fibroblasts. Microorganisms. 2022;10(12):2481. doi:10.3390/microorganisms10122481
• Ul Hassan MH, Shahbaz M, Imran M, et al. Isoflavones: promising natural agent for cancer prevention and treatment. Food Sci Nutr Mar. 2025;13(3):e70091. doi:10.1002/fsn3.70091
• Kishimoto M, Nomoto R, Mizuno M, Osawa R. An in vitro investigation of immunomodulatory properties of Lactobacillus plantarum and L. delbrueckii cells and their extracellular polysaccharides. Biosci Microbiota Food Health. 2017;36(3):101-110. doi:10.12938/bmfh.17-001