Effects of iloprost infusion on the eyes due to ovarian ischemia reperfusion ınjury: an experimental study in rats

Year 2022, Volume: 1 Issue: 3, 152 - 159, 23.12.2022

Fadime Tosun , Ebru Annaç , Mevlüt Doğukan , Mehmet Duran , Öznur Uludag

Abstract

Background: In this study, it is aimed to investigate the effect of iloprost treatment on the eye, which is a prostaglandin analogue, given to rats after the ovarian ischemia-reperfusion model and to contribute to the literature.

Materials and Methods: A total of 32 female Sprague Dawley rats were used for the experiment. Rats were divided into four groups of eight rat search: control group, ischemia group (I), ischemia-reperfusion (I-R) group, I-R/I. Except for the control group, torsion procedure was performed on all rats. 3-hour torsion in the ischemia group, 3-hour torsion and 3-hour detorsion in the I-R group, 3 hour torsion, 3 hour detorsion and 60 mn intravenous iloprost infusion in the I-R/I. After the procedures in each group, the eye tissues were taken for histopathological examination.

Results: In the histopathological examination of the corneal layer, many large gaps and (decreased collagen cross-linking) were detected between the stromal collagen fiber bundles in group I, I-R and I-R/I unlike the control group. In the histopathological examination of the retinal layer and stroma of the iris, nohemoragicarea and inflammation findings were observed in the examinations performed in all groups, but vascular dilatation was found in groups I, I-R and I-R/I when compared to the control group. A higher increase in vascular dilatation was detected in group I-R/I compared to the other groups.

Conclusions: After I/R, although histopathological changes in the eye tissue such as morphological disorders in the stromal collagen fiber bundles of the corneal layer and vascular dilatation in the stroma of iris are supported by the literature, the protective effect of iloprost on tissue damage has not been fully determined.

Keywords

Rat, Iloprost, Ischemia/Reperfusion Model, Distant Organ Damage, Eye

Over iskemi reperfüzyon hasarına karşı verilen iloprost infüzyonunun göz üzerine etkileri: ratlarda deneysel çalışma

Abstract

Amaç: Bu çalışmada, ratlara ovarial iskemi reperfüzyon (İ/R) modeli sonrası verilen prostoglandin analoğu olan iloprost tedavisinin uzak organ olarak göz üzerine etkilerini araştırmak ve literatüre katkıda bulunmak amaçlanmıştır.

Materyal ve Metot: Deney için toplam 32 dişi Sprague Dawley cinsi rat kullanıldı. Ratlar, her birinde sekiz adet olacak şekilde dört gruba ayrıldı; kontrol (K), iskemi (İ), iskemi-reperfüzyon (İ-R) ve iskemi-reperfüzyon/iloprost (İ-R/İ). Kontrol grubu hariç diğer ratlara torsiyon işlemi yapıldı. İskemi grubunda 3 saatlik torsiyon, İ-R grubuna 3 saat torsiyon ve 3 saat detorsiyon, İ-R/İ grubuna 3 saat torsiyon, 3 saat detorsiyon ve 60 dk intravenöz iloprost infüzyonu yapıldı. Her grupta işlemlerden sonra göz dokuları histopatolojik olarak incelenmek üzere alındı.

Bulgular: Kornea tabakasına ait histopatolojik incelemede Grup İ, İ-R ve İ-R/İ’ te kontrol grubundan farklı olarak stromal kollajen lif demetleri arasında çok sayıda geniş boşluklara sebep olan çapraz bağlantılarda azalma tespit edildi. Retina tabakasına ait histopatolojik incelemede tüm gruplarda yapılan incelemeler sonucunda herhangi bir hemarojik alan ve enflamasyon bulgusu gözlenmezken kontrol grubuna kıyaslandığında Grup İ, İ-R ve İ-R/İ’ te iris stromasında vasküler dilatasyon bulgusuna rastlandı. Grup İ-R/İ’te vasküler dilatasyon etkisinde diğer gruplara göre biraz daha artış tespit edildi.

Sonuç: İ/R sonrasında göz dokusunda kornea tabakasına ait stromal kollajen lif demetlerinde çapraz bağlantıda bozukluklar ve iris stroma dokusundaki vasküler dilatasyon gibi histopatolojik değişikliklerin gözlenmesi literatürle desteklenir nitelikte olmakla birlikte iloprostun doku hasarı üzerindeki koruyucu etkisi tam anlamıyla tespit edilemedi.

Keywords

Sıçan, İloprost, İskemi/Reperfüzyon Modeli, Uzak Organ Hasarı, Göz

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