Thlaspi perfoliatum L.'un Antimikrobiyal, Antifungal ve Antibiyofilm Aktiviteleri: Kapsamlı Bir In Vitro Çalışma

Year 2025, Volume: 8 Issue: 2, 324 - 334, 31.12.2025

Mehmet Cengiz Karaismailoglu , Nilsu Can , Yusuf Ceylan

https://doi.org/10.55930/jonas.1736146

Abstract

Bu çalışma, Türkiye’de halk arasında yaygın olarak kullanılan Thlaspi perfoliatum L. bitkisinin antibakteriyel, antifungal ve antibiyofilm etkilerini incelemeyi amaçlamıştır. Bitkinin toprak üstü kısımlarından metanol ekstraktı hazırlanmış ve Gram-pozitif (Bacillus subtilis, Staphylococcus aureus ve Enterococcus durans) ile Gram-negatif (Escherichia coli, Salmonella enteritidis, Enterobacter aerogenes) bakterilere karşı disk difüzyon, MIC ve MBC yöntemleri ile test edilmiştir. Antifungal etkinlik Candida albicans ve Candida utilis üzerinde değerlendirilmiş, antibiyofilm etkisi ise özellikle S. aureus üzerinde incelenmiştir. Ekstrakt, doza bağlı olarak antibakteriyel etki göstermiştir. En güçlü etki S. aureus ve E. aerogenes üzerinde gözlenmiştir. Yüksek konsantrasyonlarda anlamlı antifungal etki elde edilmiş, ayrıca S. aureus’un biyofilm oluşumu belirgin şekilde azalmıştır. Elde edilen bulgular, T. perfoliatum bitkisinin mikrobiyal büyümeyi ve biyofilm oluşumunu engelleyebilecek doğal bileşikler içerdiğini ve geleneksel kullanımının bilimsel temelleri olabileceğini göstermektedir. Thlaspi perfoliatum metanol ekstraktı antibakteriyel, antifungal ve antibiyofilm etkiler göstermiştir. Bu özellikleri sayesinde doğal bir antimikrobiyal ajan olarak değerlendirilebilecek potansiyele sahiptir.

Keywords

Antibiyofilm , Antifungal , Biyofilm inhibisyonu , Brassicaceae , Thlaspi perfoliatum

Project Number

Project number: 1919B012304847 – 2209A

ANTIMICROBIAL, ANTIFUNGAL AND ANTIBIOFILM ACTIVITIES OF Thlaspi perfoliatum L.: A COMPREHENSIVE IN VITRO STUDY

Abstract

This investigation targeted to research the antibacterial, antifungal, and antibiofilm activities of Thlaspi perfoliatum L., a plant widely used in Turkish folk medicine. Methanol extract was prepared aboveground parts of the plant and tested against Gram-positive (Bacillus subtilis, Staphylococcus aureus and Enterococcus durans) and Gram-negative (Escherichia coli, Salmonella enteritidis, Enterobacter aerogenes) bacteria using disk diffusion, MIC, and MBC methods. Antifungal activity was evaluated against Candida albicans and Candida utilis, and antibiofilm effects were examined, particularly on S. aureus. The extract showed dose-dependent antibacterial activity, with the strongest effects observed against S. aureus and E. aerogenes. Significant antifungal activity was detected at higher concentrations, and the extract also inhibited biofilm formation by S. aureus. These findings suggest that T. perfoliatum contains bioactive compounds capable of inhibiting microbial growth and biofilm formation, supporting its traditional medicinal use. The methanol extract of Thlaspi perfoliatum demonstrated antibacterial, antifungal, and antibiofilm properties, highlighting its potential as a natural antimicrobial agent

Keywords

Antibiofilm , Antifungal , Biofilm inhibition , Brassicaceae , Thlaspi perfoliatum

Ethical Statement

The authors of the articles declare that they have no conflict of interest.

Supporting Institution

the Scientific and Technological Research Council of Turkey (TÜBİTAK)

Project Number

Project number: 1919B012304847 – 2209A

Thanks

The authors are grateful to the Scientific and Technological Research Council of Turkey (TÜBİTAK) for providing financial support for this study (Project number: 1919B012304847 – 2209A).

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