Year 2020, Volume 3 , Issue 2, Pages 73 - 83 2020-12-31

INVESTIGATION on THE ANTIPROLIFERATIVE ACTIVITY OF CHLORO[1-(2-METHYL-2-PROPENYL)-3-(2-CHLOROBENZYL) BENZIMIDAZOLE-2-YLIDENE Ag(I) COMPOUND on SH-SY5Y NEUROBLASTOMA CELLS
KLORO[1-(2-METİL-2-PROPENİL)-3-(2-KLOROBENZİL) BENZİMİDAZOL-2-İLİDEN] Ag(I) BİLEŞİĞİNİN SH-SY5Y NÖROBLASTOM HÜCRELERİNDEKİ ANTİPROLİFERATİF AKTİVİTESİNİN ARAŞTIRILMASI

Batuhan YILDIZ [1] , Serap ŞAHİN BÖLÜKBAŞI [2] , Neslihan ŞAHİN [3]


In this study, it has been investigated the antiproliferative activities of benzimidazole-based NHC ligand (1) and its silver(I) complex (2) having chloride. Both ligand (1) and complex (2) have been tested for their antiproliferative activity on SH-SY5Y neuroblastoma cells and WI-38 human healthy fibroblast cells for 24 h, 48 h and 72 h using the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. According to the experimental results, ligand (1) has not shown antiproliferative activity on WI-38 healthy cells in all-time points but has shown concentration- and time-dependent antiproliferative activity on SH-SY5Y neuroblastoma cells . Our results have shown that complex (2) has a dose and time-dependent antiproliferative activity against both SH-SY5Y neuroblastoma cells and WI-38 healthy cells. It has also been found that complex (2) has more antiproliferative activity than ligand (1) on SH-SY5Y neuroblastoma cells and WI-38 healthy cells. The IC50 value of the ligand (1) towards the SH-SY5Y neuroblastoma cell line was calculated > 20, 8.90, and 4.28 µM for 24 h, 48 h, and 72 h , respectively. IC50 values of ligand (1) against WI-38 normal lung fibroblast cell lines were calculated > 20 µM for 24, 48, and 72 hours. IC50 values of the complex (2) against SH-SY5Y neuroblastoma and WI-38 normal lung fibroblast cell lines were found to be 3.80, 3.15, and 2.21 µM; and 16.9, 14.6, and 8.34 µM for 24 h, 48 h and 72 h, respectively. The selectivity index (SI) was determined from the ratio of the IC50 value obtained from normal cells and IC50 value for cancer cells. Selectivity indexes of ligand (1) and complex (2) were calculated as >1, > 2.24, and >4.67; 4.44, 4.63 and 3.77 for 24 h, 48 and 72 h, respectively. These results indicated that complex (2) has shown good selectivity (more than 4-fold) between SH-SY5Y neuroblastoma cells and normal cells for 24 h and 48 h incubation time.

Bu çalışmada klor içeren benzimidazol-temelli N-heterosiklik karben (NHC) ligandı (1) ve ligandın gümüş-(I) kompleksinin (2) antiproliferatif aktiviteleri araştırılmıştır. Hem ligand (1) hem de kompleksin (2), SH-SY5Y nöroblastom hücrelerine ve WI-38 insan sağlıklı fibroblast hücrelerindeki antiproliferatif aktiviteleri 24 saat, 48 saat ve 72 saat boyunca (3-[4,5-Dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide; Thiazolyl blue (MTT) ölçümü kullanılarak test edilmiştir. Deney sonuçlarına göre ligand (1), tüm zaman noktalarında WI-38 sağlıklı hücrelerde antiproliferatif aktivite göstermemiş ancak SH-SY5Y nöroblastom hücrelerinde konsantrasyona ve zamana bağlı antiproliferatif aktivite göstermiştir. Sonuçlarımız, kompleksin (2), hem SH-SY5Y nöroblastom hücrelerine hem de WI-38 sağlıklı hücrelerine karşı doza ve zamana bağlı bir antiproliferatif aktiviteye sahip olduğunu göstermiştir. Ayrıca kompleksin (2), SH-SY5Y nöroblastom hücreleri ve WI-38 normal hücrelerinde liganda (1) göre daha yüksek antiproliferatif aktiviteye sahip olduğu bulunmuştur. Kompleks (2), SH-SY5Y nöroblastom hücreleri için 24 ve 48 saat zaman noktalarında 4 kattan fazla seçicilik göstermiştir.

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Primary Language tr
Subjects Basic Sciences
Journal Section Articles
Authors

Orcid: 0000-0002-5868-959X
Author: Batuhan YILDIZ
Institution: SİVAS CUMHURİYET ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ, TEMEL ECZACILIK BİLİMLERİ BÖLÜMÜ, BİYOKİMYA ANABİLİM DALI
Country: Turkey


Orcid: 0000-0003-1057-2558
Author: Serap ŞAHİN BÖLÜKBAŞI (Primary Author)
Institution: SİVAS CUMHURİYET ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ, TEMEL ECZACILIK BİLİMLERİ BÖLÜMÜ, BİYOKİMYA ANABİLİM DALI
Country: Turkey


Orcid: 0000-0003-1498-4170
Author: Neslihan ŞAHİN
Institution: CUMHURİYET ÜNİVERSİTESİ, EĞİTİM FAKÜLTESİ, TEMEL EĞİTİM BÖLÜMÜ, SINIF EĞİTİMİ ANABİLİM DALI
Country: Turkey


Supporting Institution -
Project Number -
Thanks Antiproliferatif aktivite deneyleri Sivas Cumhuriyet Üniversitesi İleri Teknoloji Araştırma ve Uygulama Merkezi (CÜTAM)’da yapılmıştır.
Dates

Publication Date : December 31, 2020

Bibtex @research article { jonas721319, journal = {Bartın University International Journal of Natural and Applied Sciences}, issn = {}, eissn = {2667-5048}, address = {Bartın Üniversitesi Fen Bilimleri Enstitüsü Ağdacı Kampüsü 74100-BARTIN}, publisher = {Bartin University}, year = {2020}, volume = {3}, pages = {73 - 83}, doi = {}, title = {KLORO[1-(2-METİL-2-PROPENİL)-3-(2-KLOROBENZİL) BENZİMİDAZOL-2-İLİDEN] Ag(I) BİLEŞİĞİNİN SH-SY5Y NÖROBLASTOM HÜCRELERİNDEKİ ANTİPROLİFERATİF AKTİVİTESİNİN ARAŞTIRILMASI}, key = {cite}, author = {Yıldız, Batuhan and Şahin Bölükbaşı, Serap and Şahin, Neslihan} }
APA Yıldız, B , Şahin Bölükbaşı, S , Şahin, N . (2020). KLORO[1-(2-METİL-2-PROPENİL)-3-(2-KLOROBENZİL) BENZİMİDAZOL-2-İLİDEN] Ag(I) BİLEŞİĞİNİN SH-SY5Y NÖROBLASTOM HÜCRELERİNDEKİ ANTİPROLİFERATİF AKTİVİTESİNİN ARAŞTIRILMASI . Bartın University International Journal of Natural and Applied Sciences , 3 (2) , 73-83 . Retrieved from https://dergipark.org.tr/en/pub/jonas/issue/57209/721319
MLA Yıldız, B , Şahin Bölükbaşı, S , Şahin, N . "KLORO[1-(2-METİL-2-PROPENİL)-3-(2-KLOROBENZİL) BENZİMİDAZOL-2-İLİDEN] Ag(I) BİLEŞİĞİNİN SH-SY5Y NÖROBLASTOM HÜCRELERİNDEKİ ANTİPROLİFERATİF AKTİVİTESİNİN ARAŞTIRILMASI" . Bartın University International Journal of Natural and Applied Sciences 3 (2020 ): 73-83 <https://dergipark.org.tr/en/pub/jonas/issue/57209/721319>
Chicago Yıldız, B , Şahin Bölükbaşı, S , Şahin, N . "KLORO[1-(2-METİL-2-PROPENİL)-3-(2-KLOROBENZİL) BENZİMİDAZOL-2-İLİDEN] Ag(I) BİLEŞİĞİNİN SH-SY5Y NÖROBLASTOM HÜCRELERİNDEKİ ANTİPROLİFERATİF AKTİVİTESİNİN ARAŞTIRILMASI". Bartın University International Journal of Natural and Applied Sciences 3 (2020 ): 73-83
RIS TY - JOUR T1 - KLORO[1-(2-METİL-2-PROPENİL)-3-(2-KLOROBENZİL) BENZİMİDAZOL-2-İLİDEN] Ag(I) BİLEŞİĞİNİN SH-SY5Y NÖROBLASTOM HÜCRELERİNDEKİ ANTİPROLİFERATİF AKTİVİTESİNİN ARAŞTIRILMASI AU - Batuhan Yıldız , Serap Şahin Bölükbaşı , Neslihan Şahin Y1 - 2020 PY - 2020 N1 - DO - T2 - Bartın University International Journal of Natural and Applied Sciences JF - Journal JO - JOR SP - 73 EP - 83 VL - 3 IS - 2 SN - -2667-5048 M3 - UR - Y2 - 2020 ER -
EndNote %0 Bartın Üniversitesi Uluslararası Fen Bilimleri Dergisi KLORO[1-(2-METİL-2-PROPENİL)-3-(2-KLOROBENZİL) BENZİMİDAZOL-2-İLİDEN] Ag(I) BİLEŞİĞİNİN SH-SY5Y NÖROBLASTOM HÜCRELERİNDEKİ ANTİPROLİFERATİF AKTİVİTESİNİN ARAŞTIRILMASI %A Batuhan Yıldız , Serap Şahin Bölükbaşı , Neslihan Şahin %T KLORO[1-(2-METİL-2-PROPENİL)-3-(2-KLOROBENZİL) BENZİMİDAZOL-2-İLİDEN] Ag(I) BİLEŞİĞİNİN SH-SY5Y NÖROBLASTOM HÜCRELERİNDEKİ ANTİPROLİFERATİF AKTİVİTESİNİN ARAŞTIRILMASI %D 2020 %J Bartın University International Journal of Natural and Applied Sciences %P -2667-5048 %V 3 %N 2 %R %U
ISNAD Yıldız, Batuhan , Şahin Bölükbaşı, Serap , Şahin, Neslihan . "KLORO[1-(2-METİL-2-PROPENİL)-3-(2-KLOROBENZİL) BENZİMİDAZOL-2-İLİDEN] Ag(I) BİLEŞİĞİNİN SH-SY5Y NÖROBLASTOM HÜCRELERİNDEKİ ANTİPROLİFERATİF AKTİVİTESİNİN ARAŞTIRILMASI". Bartın University International Journal of Natural and Applied Sciences 3 / 2 (December 2020): 73-83 .
AMA Yıldız B , Şahin Bölükbaşı S , Şahin N . KLORO[1-(2-METİL-2-PROPENİL)-3-(2-KLOROBENZİL) BENZİMİDAZOL-2-İLİDEN] Ag(I) BİLEŞİĞİNİN SH-SY5Y NÖROBLASTOM HÜCRELERİNDEKİ ANTİPROLİFERATİF AKTİVİTESİNİN ARAŞTIRILMASI. JONAS. 2020; 3(2): 73-83.
Vancouver Yıldız B , Şahin Bölükbaşı S , Şahin N . KLORO[1-(2-METİL-2-PROPENİL)-3-(2-KLOROBENZİL) BENZİMİDAZOL-2-İLİDEN] Ag(I) BİLEŞİĞİNİN SH-SY5Y NÖROBLASTOM HÜCRELERİNDEKİ ANTİPROLİFERATİF AKTİVİTESİNİN ARAŞTIRILMASI. Bartın University International Journal of Natural and Applied Sciences. 2020; 3(2): 73-83.
IEEE B. Yıldız , S. Şahin Bölükbaşı and N. Şahin , "KLORO[1-(2-METİL-2-PROPENİL)-3-(2-KLOROBENZİL) BENZİMİDAZOL-2-İLİDEN] Ag(I) BİLEŞİĞİNİN SH-SY5Y NÖROBLASTOM HÜCRELERİNDEKİ ANTİPROLİFERATİF AKTİVİTESİNİN ARAŞTIRILMASI", Bartın University International Journal of Natural and Applied Sciences, vol. 3, no. 2, pp. 73-83, Dec. 2021