Fiber optik biyoimpedans spektroskopi sistemi ile meme hücrelerinde kanser gelişiminin belirlenmesi

Öz

Amaç: Kanserin erken evrede teşhis edildiğinde en etkili şekilde tedavi edilebileceği iyi bilinmektedir. Bu nedenle, kanserin ve prekanseröz lezyonların erken tespiti için yeni biyomedikal yaklaşımların geliştirilmesi, değerlendirilmesi ve validasyonu önemli bir önceliktir. Amacımız, Fiber Optik Biyoimpedans Spektroskopisi (FOBIS) sistemini kullanarak düşük metastatik insan meme hücrelerini normal insan meme hücrelerinden ayırt etmekti.

Yöntemler: FOBİS sisteminde 50µm çaplı fiberler ve 25µm çaplı platin teller kullanılmıştır. Sistemin farklı hücre tiplerini ayırt etme duyarlılığı yüksek metastatik (MDA-MB-231), düşük metastatik (MCF-7) ve normal meme epitel hücreleri (MCF-10A) için hesaplanmıştır. Verilerin istatistiksel değerlendirmesi Temel Bileşenler Analizi (TBA) ve Doğrusal Ayırım Analizi (DAA) ile yapılmıştır. İnsan meme hücre kültürlerinde FOBİS sistemi ile elde edilen spektroskopik veriler, çok değişkenli istatistiksel analiz ile değerlendirilerek hücre tipi hakkında bilgi elde edilmiştir. Fiber optik ve biyoimpedans yöntemlerinden elde edilen spektroskopik veriler ile farklı hücre tipleri ayırt edilebilmektedir. Bu iki tekniğin birleştirilmesi ile sistemin insan meme hücrelerinin farklılaşmasına duyarlılığı test edilmiştir.

Bulgular: Buna göre %100 duyarlılık ve %60 seçicilik ile MCF-7 hücreleri MCF-10A hücrelerinden ayırt edilmiştir. 

Sonuç: FOBİS sistemi ile hücre kültüründe meme kanseri hücreleri yüksek duyarlılıkla ayırt edilmiştir.

Anahtar Kelimeler

• Biyoimpedans,Meme,Hücre ayırımı,Fiber optik,Spektroskopi

Determination of cancer progression in breast cells by fiber optic bioimpedance spectroscopy system

Abstract

Aim: It is well established that cancer can be most effectively treated when diagnosed at an early stage. Therefore, development, evaluation, and validation of new biomedical approaches for early detection of cancer and precancerous lesions are important priorities. Our aim was to distinguish low metastatic human breast cells from normal human breast cells using the Fiber Optic Bioimpedance Spectroscopy (FOBIS) system.

Methods: In the FOBIS system we developed, the diameters of the fibers and platinum wires are 50 and 25µm, respectively. The sensitivity of the system to differentiate different cell types was assessed with high metastatic (MDA-MB-231), low metastatic (MCF-7) and normal breast epithelial cells (MCF-10A). Statistical evaluation of data was performed by using Principle Component Analysis (PCA) and Linear Discriminant Analysis (LDA). Spectroscopic data obtained from FOBIS system on suspended human breast cells were evaluated by multivariate statistical analysis to obtain information about the cell type. Fiber optic and bioimpedance methods allow discrimination of different cell types based on their signature. By combining these two techniques, the sensitivity of the system to the differentiation of human breast cells was evaluated.

Results: The discrimination provided the sensitivity of 100% and specificity of 60% in distinguishing MCF-7 from MCF-10A cells.

Conclusion: A highly accurate distinction of breast cancer cells was achieved in cell culture by FOBIS system.

Keywords

• Bioimpedance,Breast,Cell Distinguish,Fiber Optic,Spectroscopy

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