Ozon terapinin postoperatif adezyon ve over fonksiyonları üzerindeki etkisi: Deneysel bir çalışma

Öz

Amaç: Postoperatif adezyon gelişiminin önlenmesi için çok sayıda yöntem kullanılmaktadır. Ozon tedavisinin postoperatif batın içi adezyonlar ve over fonksiyonları üzerine etkileriyle ilgili literatürde az sayıda çalışma mevcuttur. Bu çalışma, ozon tedavisinin ratlarda postoperatif batın içi adezyonlar ve over fonksiyonları açısından etkilerini araştırmayı amaçlamıştır. 

Yöntemler: Yirmi adet dişi Wistar albino rat, laparotomi (Grup 1, n:10) ve laparotomi + intraperitoneal ozon (Grup 2, n:10) olacak şekilde rastgele iki gruba ayrıldı. Laparotomi sonrası her iki grupta parietal periton, uterus ve adneksiyal yüzeyler kazındı. Folikül uyarıcı hormon (FSH), Estradiol (E2) ve Laktat Dehidrojenaz (LDH), Üre ve Kreatinin seviyeleri ölçüldü, histopatolojik değerlendirme yapıldı. Her iki grup histopatolojik ve biyokimyasal bulgular açısından karşılaştırıldı.

Bulgular: Ozon tedavi grubunda antioksidan seviyeleri (P=0,012), kontrol grubuna göre anlamlı derecede yüksek, E2 seviyeleri (P=0,005) ise anlamlı derecede düşük bulundu. İki grup arasında FSH, Üre, Kreatinin, LDH açısından istatistiksel olarak anlamlı fark bulunmadı (sırasıyla P=0,12, P=0,72, P=0,45, P=0,79). Histopatolojik olarak her iki grup arasında postoperatif intraabdominal adezyon oranları istatistiksel olarak benzer saptandı. 

Sonuç: Her ne kadar istatistiksel olarak anlamlı fark olmasa da ozon terapinin şiddetli fibrozis ve konjesyon oranlarında azaltıcı etkisinin olduğu tespit edildi. FSH açısından fark bulunmasa da Grup2’de E2 seviyelerinin düşük tespit edilmesi, ozon tedavisinin over fonksiyonları üzerinde koruyucu etki oluşturabileceğini akla getirmektedir. Ancak adezyon oluşumu ve over fonksiyonları üzerindeki etkiler açısından daha kapsamlı çalışmalara ihtiyaç vardır.

Anahtar Kelimeler

• Rat,Ozon,Adezyon

The effects of ozone therapy on postoperative adhesions and ovarian functions: An experimental study

Abstract

Aim: Numerous methods are used to prevent the development of postoperative adhesion formation. There are few studies on the effects of ozone therapy on postoperative intraabdominal adhesions and ovarian functions. This study aimed to investigate the effects of ozone treatment on postoperative intraabdominal adhesions and ovarian roles in rats.

Methods: Twenty female Wistar albino rats were randomly allocated into two groups as laparotomy (Group 1, n: 10) and laparotomy + intraperitoneal ozone (Group 2, n: 10). After laparotomy, parietal peritoneum, uterus and adnexal surfaces were scraped in both groups. Follicle-stimulating hormone (FSH), Estradiol (E2), Lactate Dehydrogenase (LDH), Urea, and Creatinine levels were measured, and histopathological evaluation was performed. Both groups were compared concerning histopathological and biochemical findings.

Results: In the ozone-treated group, antioxidant levels (P=0.012) were significantly higher, and E2 levels (P=0.005) were substantially lower than the control group. There was no statistically significant difference between the two groups regarding FSH, Urea, Creatinine, and LDH (P=0.12, P=0.72, P=0.45, and P=0.79, respectively). Histopathologically, postoperative intraabdominal adhesion rates between the two groups were statistically similar.

Conclusion: Although there was no statistically significant difference, ozone therapy had a decreasing effect on severe fibrosis and congestion rates. Although there was no difference in FSH, low levels of E2 in Group 2 suggest that ozone treatment may have a protective effect on the ovaries. However, further studies are needed concerning adhesion formation and the impact on ovarian functions.

Keywords

• Rats,Ozone,Adhesion

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