Serum RANKL levels and bioelectric impedance assessments in knee osteoarthritis patients

Year 2020, , 222 - 225, 01.03.2020

Elif Pala , Tuba Denkçeken , Türkan Turgay

https://doi.org/10.28982/josam.697686

Abstract

Aim: Osteoarthritis (OA) is a common joint disease that is caused by mechanical, genetic, and biochemical factors, and knee OA is one of the reasons of mobility limitation and disability. The receptor activator of NF-κB ligand (RANKL) is directly involved in the differentiation of osteoclasts through its receptor RANK. In this study, we aimed to study circulating serum levels of RANKL, and assess knee bioelectric impedance in control and patients with knee OA.
Methods: In this case-control study, OA severity was evaluated by the Kellgren–Lawrence grading scale, based on which we categorized patient groups. There were 22 control individuals (Grades 0 and 1), 11 early (Grade 2) and 30 late OA patients (Grades 3 and 4). We evaluated the performance of the bioimpedance phase angle values at 50 kHz. The RANKL protein levels in the serum were quantified using Enzyme-Linked Immunosorbent Assay (ELISA).
Results: It was observed that the control group could not be differentiated from the study group by using phase angle values (P=0.925). Concerning the RANKL levels, although it shows a relative increase in the study group, it did not reach a significant level (P=0.116).
Conclusion: The phase angle values at 50 kHz and RANKL levels may not be used in predictive detection of knee OA. Additional studies with larger sample sizes are needed to interpret if these changes are consistent and clinically related. 

Keywords

RANKL, Bioimpedance, Knee osteoarthritis

Diz osteoartritli hastalarda serum RANKL düzeyleri ve biyoelektrik empedans değerlendirmeleri

Abstract

Amaç: Osteoartrit (OA), mekanik, genetik ve biyokimyasal faktörlerin etkilerinden kaynaklanan yaygın bir eklem hastalığıdır ve diz OA’i, hareket kısıtlaması ve engel nedenlerinden biridir. Bu çalışmada NF-κB ligandının (RANKL) reseptör aktivatörünün dolaşımdaki serum seviyelerini incelemeyi ve kontrolde ve diz OA'i olan hastalarda biyoelektrik empedansını değerlendirmeyi amaçladık.
Yöntemler: Çalışmamız bir vaka-kontrol çalışması olarak tasarlanmıştır. OA şiddeti Kellgren-Lawrence derecelendirme skalası ile değerlendirildi, gruplar 22 sağlıklı kontrol (derece 0 ve 1), ve çalışma grubu 11 erken (derece 2) ve 30 geç OA (derece 3 ve 4) olarak tanımlandı. 50kHz’te biyoimpedans faz açısı değerlendirildi. Serumdaki RANKL protein seviyeleri Enzyme Linked Immunosorbent Assay (ELISA) metodu ile ölçüldü.
Bulgular: Kontrol grubunun faz açısı değerleri kullanılarak diz OA grubundan ayırt edilemediği gözlendi (P=0,925). RANKL düzeyleri ile ilgili olarak, çalışma grubunda göreceli bir artış tespit edilse de, anlamlı bir seviyeye ulaşmadı (P=0,116).
Sonuç: 50 kHz faz açısı değerleri ve RANKL seviyeleri, diz OA'sının tespitinin öngörülmesinde kullanılamayabileceği belirlenmiştir. Bu değişikliklerin tutarlı ve klinik olarak ilişkili olup olmadığını yorumlamak için daha büyük örnek büyüklüklerine sahip ek araştırmalara ihtiyaç vardır.

Keywords

RANKL, Biyoimpedans, Diz osteoartiriti

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